Vascularization in bone tissue engineering constructs

骨组织工程结构中的血管化

基本信息

批准号：
10552011
负责人：
YUNZHI YANG
金额：
$ 34.6万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-02-06 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10552011
关键词：
3D Print Acceleration Address Anatomy Area Autologous Transplantation Beds Biocompatible Materials Bioreactors Blood Vessels Blood flow Bone Regeneration Bone Tissue Bone Transplantation Cells Clinical Clinical Treatment Collagen Complex Defect Diffusion Endothelium Engineering Engraftment Ensure Environment Failure Feasibility Studies Femur Geometry Goals Growth Factor Hydrogels In Vitro Mechanics Methods Microsurgery Modeling Operative Surgical Procedures Orthopedic Surgery Osteogenesis Permeability Pharmaceutical Preparations Polymers Porosity Property Rattus Site Surgical Anastomosis Technology Testing Tissue Engineering Tissue Grafts Tissue constructs Tissues Translations Vascularization Work angiogenesis blood perfusion bone bone engineering calcium phosphate design effectiveness evaluation fabrication flexibility functional restoration graft function implantation improved in vivo mechanical properties neovascularization novel physical property polycaprolactone prototype release factor repaired scaffold success thrombotic

项目摘要

Abstract Treatment of segmental large bone defects remains a significant clinical problem. Pre- vascularized large bone grafts capable of rapidly anastomosing with host vessel bed and integrating with native bone tissue would significantly advance currently available treatments. Addressing these issues is expected to overcome one of the stumbling blocks of tissue engineering and revolutionize the treatment of large bone defects. In this project, we propose to engineer a novel pre-vascularized bone graft substitute to repair large bone defects. The novel bone graft is comprised of a channeled, biodegradable calcium phosphate-polymer composite scaffold containing a cell-laden hydrogel (as a bone surrogate) and a flexible, suturable, antithrombotic, permeable polymer-based tissue engineered vessel graft (TEVG, as a blood vessel surrogate). The TEVG will be incorporated into the scaffold in a spatially controlled fashion. Our hypothesis is that the TEVG will facilitate and promote microvascularization across the scaffold, while allowing for surgical anastomosis to enable instant integration between the engineered bone graft and host tissue to repair large bone defects. To test our hypothesis, our team comprised of experts in biomaterials, bone tissue engineering, neovascularization, micro- surgery and orthopedic surgery, proposes the following aims:!Aim 1 is to develop a TEVG that facilitates host vascular integration. Aim 2 is to design and develop an instantly integrated, pre- vascularized bone graft substitute. Aim 3 is to determine the efficiency of a vascularized bone tissue construct prototype to enhance integration with host bone tissue, accelerate bone regeneration and restore functionality. The accomplishment of this project will revolutionize vascularized synthetic bone graft design, and pave the way for improved clinical treatments for large segmental bone defects. !

抽象的节段性大骨缺损的治疗仍然是一个重要的临床问题。预血管化的大骨移植物能够与宿主血管床快速吻合，与天然骨组织的整合将显着推进目前可用的治疗方法。解决这些问题有望克服组织的绊脚石之一工程设计并彻底改变大骨缺损的治疗。在这个项目中，我们建议设计一种新型的预血管化骨移植替代品来修复大骨缺损。小说骨移植物由可生物降解的通道式磷酸钙聚合物复合材料组成支架包含充满细胞的水凝胶（作为骨替代物）和柔性的、可缝合的、抗血栓、可渗透聚合物基组织工程血管移植物（TEVG，作为血液血管替代品）。 TEVG 将以空间受控的方式整合到脚手架中时尚。我们的假设是 TEVG 将促进和促进微血管化支架，同时允许手术吻合，以实现支架之间的即时整合工程骨移植和宿主组织可修复大骨缺损。为了检验我们的假设，我们的团队由生物材料、骨组织工程、新生血管、微血管等领域的专家组成外科和骨科手术，提出以下目标：！目标 1 是开发一种 TEVG 促进宿主血管整合。目标 2 是设计和开发一个即时集成的、预血管化骨移植替代品。目标 3 是确定血管化骨的效率组织构建原型，增强与宿主骨组织的整合，加速骨形成再生和恢复功能。这个项目的完成将带来革命性的变化血管化合成骨移植设计，并为改善临床治疗铺平道路大节段性骨缺损。！