Animal Core

动物核心

基本信息

批准号：
10549478
负责人：
Stephen Mark Tompkins
金额：
$ 39.03万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-21 至 2028-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10549478
关键词：
2019-nCoV Animal Experimentation Animal Model Animals Antibodies Antibody Therapy B-Lymphocytes Biological Assay Cattle Communities Coronavirus Coronavirus Infections Data Disease Doctor of Philosophy Ferrets Goals Hamsters Health Housing Human Immune Immunity Immunological Models Individual Infection Merbecovirus Middle East Respiratory Syndrome Coronavirus Modeling Monoclonal Antibodies Mus National Institute of Allergy and Infectious Disease Outcome Pliability Productivity Protocols documentation RNA vaccination Research Research Personnel Research Project Grants Resources Respiratory Tract Infections Running SARS coronavirus SARS-CoV-2 variant Sampling Sarbecovirus Serum Services Testing Therapeutic Monoclonal Antibodies Therapeutic antibodies Transgenic Mice Universities Vaccination Vaccine Therapy Vaccines Variant Veterinary Medicine Viral Virus Work animal coronavirus animal resource betacoronavirus betacoronavirus vaccine college coronavirus disease coronavirus vaccination coronavirus vaccine effectiveness evaluation efficacy evaluation efficacy study experience high risk human disease immunological diversity imprint improved innovation lead candidate meetings neutralizing monoclonal antibodies novel novel coronavirus pathogen pre-clinical assessment prevent programs respiratory virus response seasonal coronavirus success synergism therapeutic candidate tool training opportunity transmission process universal coronavirus vaccine vaccine candidate vaccine development vaccine efficacy vaccine-induced antibodies virus resource

项目摘要

Summary/Abstract Robust animal models of coronavirus infection are required for pre-clinical assessment of candidate vaccines and therapeutic antibodies to prevent coronavirus infection and disease. The goal of the Animal Model Core is to provide a central resource of animal models and virological tools to assess the effectiveness of vaccines and antibody therapies against betacoronaviruses in multiple animal models of infection. An experienced team of investigators will utilize established hamster, mouse, and ferret models of betacoronavirus infection to determine the efficacy of candidate therapeutic monoclonal antibodies (mAbs) developed by Project 1 and vaccines developed by Project 2. Pre-immune and transmission models will be utilized to stringently test the lead candidate vaccines. The Animal Model Core will also establish novel animal infection models with SARS- CoV-2 variant viruses, and a novel ferret model of OC43 embecovirus infection. The Animal Model Core will also complete virus neutralization assays for SARS-CoV and MERS CoV for all PLUTO investigators, centralizing resources for these viruses, eliminating redundant biosafety and Select Agent approvals. The Animal Model Core will serve as an innovative resource to assess efficacy of leading vaccine and mAb candidates for the PLUTO program through the following three Specific Aims. 1) Establish and maintain animal models of sarbecovirus and merbecovirus infection. 2) Establish and maintain coronavirus pre-immune animal models. 3) Expand and improve animal models of embecovirus and sarbecovirus infection. These complementary aims will enable rigorous assessment of vaccines and mAbs developed by the PLUTO Projects and develop new animal models of betacoronavirus infection for the project and respiratory virus research community. The novel pre-immune models will also provide data and samples to improve understanding of the impact of pre-existing betacoronavirus immunity on subsequent vaccination and infection. The outcomes of the Animal Model Core will have a positive impact on the success of the PLUTO program and provide novel information and research tools for the broader research community.

摘要/摘要冠状动脉疫苗的临床前评估需要强大的冠状病毒感染动物模型以及预防冠状病毒感染和疾病的治疗抗体。动物模型核心的目标是提供动物模型和病毒学工具的主要资源来评估疫苗的有效性在多种感染动物模型中，针对贝塔曲霉病毒的抗体疗法。一支经验丰富的团队研究人员将利用Betacoronavirus感染的已建立的仓鼠，小鼠和雪貂模型确定项目1和项目2开发的疫苗。将使用免疫前和传输模型严格测试铅候选疫苗。动物模型核心还将建立具有SARS-的新型动物感染模型 COV-2变体病毒和OC43 Embecovirus感染的新型雪貂模型。动物模型核心将还完成了所有冥王星研究者的SARS-COV和MERS COV的中和测定这些病毒的集中资源，消除了冗余生物安全和精选代理批准。这动物模型核心将作为评估领先疫苗和MAB功效的创新资源通过以下三个特定目标，冥王星计划的候选人。 1）建立和维护 SARBECOVIRUS和MERBECOVIRUS感染的动物模型。 2）建立和维持冠状病毒前免疫动物模型。 3）扩展和改善胚胎病毒和SARBECOVIRUS感染的动物模型。这些互补的目标将使冥王星开发的疫苗和mAB进行严格评估项目并开发了该项目和呼吸道病毒的Betacoronavirus感染的新动物模型研究社区。新颖的免疫前模型还将提供数据和样品以改进理解现有的betacoronavirus免疫对随后的疫苗接种和感染的影响。动物模型核心的结果将对冥王星计划的成功产生积极影响并为更广泛的研究社区提供新颖的信息和研究工具。