The Role of the Vocal Ligament in Vocalization

声带韧带在发声中的作用

• 批准号: 10543427
• 负责人: INGO R TITZE
• 金额: $ 56.76万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10543427
• 关键词: Address; Age; Attention; Biological; Bioreactors; Birds; Birth; Canis familiaris; Cartilage; Collagen; Collagen Fiber; Computer Models; Crico-arytenoid joint; Cricothyroid joint; Deglutition; Deposition; Development; Dose; Elastin Fiber; Elements; Exercise; Exhibits; Fiber; Fibroblasts; Frequencies; Gel; Gender; Geometry; Growth Factor; Human; In Vitro; Larynx; Length; Lifting; Ligaments; Longevity; Maintenance; Mammals; Measures; Mechanical Stress; Mechanics; Modeling; Molecular; Morphology; Muscle; Persons; Phonation; Physiological; Play; Population; Positioning Attribute; Posture; Primates; Process; Property; Protocols documentation; Public Health; Regimen; Research; Role; Rotation; Social Interaction; Specimen; Speech; Stress; Stretching; Structure; Supplementation; Techniques; Testing; Thick; Thyroarytenoid Muscle; Time; Tissue Engineering; Tissues; Translations; Variant; Voice; Voice Training; Yawning; adduct; aged; density; design; experimental study; human subject; ligament development; macula; mechanical force; microscopic imaging; pressure; response; vibration; vocal cord; vocalization; voice therapy

PROJECT SUMMARY/ABSTRACT This research addresses an important public health concern, the development and maintenance of adequate pitch variation and vocal clarity throughout the life span. Evidence has been growing that vocal pitch range and stability of vocalization can be maintained with voice training and therapy techniques that rely heavily on repeated pitch glides, presumably to stretch the vocal ligament. It is impossible to measure directly the time-course of remodeling the vocal ligament with exercise in live humans. However, strong inferences can be drawn from structural and mechanical diversity across several species, from tissue engineering, and from computational modeling. In this proposal, we combine (1) microscopic imaging to test collagen density and fiber orientation in multiple species, (2) bioreactor design of a vocal ligament with combined axial and vibrational stress and selected growth factors, (3) finite-element computer modeling to predict fundamental frequency ranges and stability in vocal fold adduction, and (4) human subject exercise protocols to increase fundamental frequency range and vocal adduction control. While the aims can all be executed independently, they cross-validate each other on the basic premise that the vocal ligament is critical for fo range and can change over time. A first hypothesis is that the ligament can sustain high tension (up to 1 MPa) for high fundamental frequencies. A second hypothesis is that the vocal ligament serves as a stabilizer in vocal fold posturing and maintaining a consistently straight fold edge for glottal closure. Doses of repeated stretching are designed for engineering tissue in vitro and for human subject exercises. Results will benefit a large population of people who are vocally active but whose voices appear physiologically weak or aged.

项目摘要/摘要 这项研究解决了一个重要的公共卫生问题，发展和维护 在整个生命周期中，足够的音高变化和声音清晰度。证据已经存在 通过语音训练可以保持声音音高范围和发声的稳定性 和治疗技术严重依赖于反复的音高滑行，大概是为了拉伸 人声韧带。不可能直接测量重塑声音的时间顺序 韧带在活人中运动。但是，可以从结构上得出强烈的推论 以及几种物种的机械多样性，从组织工程和 计算建模。在此提案中，我们将（1）微观成像结合起来测试胶原蛋白 多种物种的密度和纤维取向，（2）发声韧带的生物反应器设计 轴向和振动应力以及选定的生长因子，（3）有限元计算机 建模以预测声带内收集的基本频率范围和稳定性，（4） 人类主题锻炼方案以增加基本频率范围和人声收缩 控制。虽然目标都可以独立执行，但它们在 声音韧带对FO范围至关重要的基本前提，并且可以随着时间的流逝而变化。第一个 假设是韧带可以维持高张力（最高1 MPa）的高基础 频率。第二个假设是声音韧带在声带中充当稳定器 姿势和维护始终如一的直折边缘，以进行闭合。重复剂量 拉伸设计用于体外工程组织和人类受试者锻炼。结果 将使大量人声活跃但声音出现的人受益 生理上弱或老化。