Molecular Transducers of Physical Activity Clinical Centers (U01) - UT Health San Antonio Clinical Center

身体活动分子传感器临床中心 (U01) - UT Health 圣安东尼奥临床中心

• 批准号: 10531636
• 负责人: Nicolas Musi
• 金额: $ 180.07万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-06 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10531636
• 关键词: Molecular; Transducers; Physical; Activity; Clinical

Project Summary/Abstract: This application is for the formation of an Adult Clinical Center in response to RFA-RM-15-015 “Molecular Transducers of Physical Activity Clinical Centers (U01)” (MoTrPAC). Physical activity is an important lifestyle behavior that has been shown to be associated with morbidity and mortality from numerous chronic diseases. This has led to current public health recommendations of 150 min/wk of moderate-intensity physical activity or 75 min/wk of vigorous physical activity to achieve significant health- related benefits, and for recommendations to engage in resistance exercise. Despite the abundance of data to support physical activity as a key lifestyle factor that can prevent, delay, or reverse the onset of a variety of health-related conditions, the specific underlying physiological mechanisms and pathways by which this occurs are not fully established. Our team of investigators is well-positioned to be an Adult Clinical Center and achieve the goals and objectives outlined in this RFA. We have the capacity in Pittsburgh to recruit, retain, train, and assess the 450 participants that are required at each Adult Clinical Center. We will recruit 450 participants at the Pittsburgh Adult Clinical Center, which will include untrained subjects who will be randomized to the intervention (72% of the sample, N=324) or control condition (12% of the sample, N=54) and trained subjects (16% of the sample, N=72). Untrained participants will be randomized to one of the following for a period of 12 weeks: 1) Control (UN- CON) that will maintain their current level of physical activity; 2) Moderate-Intensity Physical Activity (UN-MOD) that will progress to 150 min/wk of supervised moderate-intensity endurance physical activity; 3) Vigorous- Intensity Physical Activity (UN-VIG) that will progress to 75 min/wk of supervised vigorous-intensity endurance physical activity; 4) Resistance Training (UN-RES) that will engage in supervised resistance exercise. In addition, exercise trained participants (50% endurance trained, 50% resistance trained) will be recruited and will undergo baseline testing only. Thus, we propose to form an Adult Clinical Center at the University of Pittsburgh to achieve the following objectives of MoTrPAC: 1. To recruit, retain, and collect data on 450 participants (378 untrained and 72 trained) at the Pittsburgh site that will contribute to the 2,700 total participants across the Adult Clinical Centers. 2. To demonstrate the effectiveness of our training program, we will compare changes in cardiorespiratory fitness in untrained participants randomized to 12-weeks of moderate-intensity endurance exercise training (UN-MOD), vigorous-intensity endurance exercise training (UN-VIG), resistance training (UN-RES) or control (UN-CON). 3. To demonstrate the effectiveness of our training program, we will compare changes in musculoskeletal fitness in untrained participants randomized to 12-weeks of UN-MOD, UN-VIG, UN-RES, or UN-CON. 4. To compare cardiorespiratory fitness in participants recruited as endurance trained to participants randomized to UN-MOD and UN-VIG following 12 weeks of training. 5. To compare musculoskeletal fitness in participants recruited as endurance trained to participants randomized to UN-RES following 12 weeks of training. 6. To collect phenotypic data that will allow for characterization of the study participants. These data will be provided to the Coordinating Center and utilized by the Bioinformatics Center, Metabolomics and Proteomics Analysis Sites, and Genomics, Epigenomics, and Transcriptomics Analysis Sites. 7. To collect biological data according to the study protocol that will provide data to examine the molecular and cellular transducers in response to acute and chronic exposure to endurance or resistance exercise training. These data will be provided to the Coordinating Center and utilized by the Bioinformatics Center, Metabolomics and Proteomics Analysis Sites, and Genomics, Epigenomics, and Transcriptomics Sites.

项目摘要/摘要： 本申请旨在响应 RFA-RM-15-015“分子 身体活动临床中心传感器 (U01)” (MoTrPAC)。 身体活动是一种重要的生活方式行为，已被证明与发病率和 这导致目前的公共卫生建议为每周 150 分钟。 中等强度的体力活动或每周 75 分钟的剧烈体力活动，以实现显着的健康- 尽管有大量数据，但仍提供了相关的好处，以及进行抵抗运动的建议。 支持身体活动作为关键的生活方式因素，可以预防、延迟或逆转多种疾病的发生 与健康相关的状况、发生这种情况的具体潜在生理机制和途径 尚未完全建立。 我们的研究团队有能力成为成人临床中心并实现目标和 本 RFA 中概述的目标 我们在匹兹堡有能力招募、保留、培训和评估这 450 名员工。 每个成人临床中心所需的参与者 我们将在匹兹堡成人中心招募 450 名参与者。 临床中心，其中将包括未经训练的受试者，他们将被随机接受干预（72% 样本，N=324）或对照条件（样本的 12%，N=54）和受过训练的受试者（样本的 16%，N=72）。 未经培训的参与者将被随机分配到以下其中一项，为期 12 周：1) 对照（UN- 2) 中等强度体力活动（UN-MOD） 将在监督下进行每周 150 分钟的中等强度耐力身体活动 3) 剧烈- 强度体力活动 (UN-VIG) 将在监督下进行每周 75 分钟的高强度耐力活动 体力活动； 4) 进行有人监督的阻力训练 (UN-RES)。 此外，将招募经过运动训练的参与者（50% 耐力训练，50% 阻力训练），并将 仅进行基线测试。 因此，我们建议在匹兹堡大学成立成人临床中心，以实现以下目标 MoTrPAC 的目标： 1. 在匹兹堡站点招募、保留和收集 450 名参与者（378 名未经培训和 72 名经过培训）的数据 这将使成人临床中心的参与者总数达到 2,700 名。 2. 为了证明我们训练计划的有效性，我们将比较心肺功能的变化 未经训练的参与者随机接受为期 12 周的中等强度耐力运动训练 (UN-MOD)、高强度耐力运动训练 (UN-VIG)、阻力训练 (UN-RES) 或对照 （联合国大会）。 3. 为了证明我们训练计划的有效性，我们将比较肌肉骨骼的变化 未经训练的参与者随机接受为期 12 周的 UN-MOD、UN-VIG、UN-RES 或 UN-CON 的健康状况。 4. 比较招募参加耐力训练的参与者的心肺健康状况 经过 12 周的培训后，随机分配至 UN-MOD 和 UN-VIG。 5. 比较招募进行耐力训练的参与者与随机参与者的肌肉骨骼健康状况 经过 12 周的培训后，将提交给 UN-RES。 6. 收集表型数据以表征研究参与者的特征。 提供给协调中心并由生物信息学中心、代谢组学和蛋白质组学使用 分析位点以及基因组学、表观基因组学和转录组学分析位点。 7. 根据研究方案收集生物数据，这将提供数据以检查分子和 细胞传感器响应急性和慢性耐力或阻力运动训练。 这些数据将提供给协调中心并由生物信息学中心使用， 代谢组学和蛋白质组学分析站点，以及基因组学、表观基因组学和转录组学站点。