Sensing local nano-environment with coherent Raman microspectroscopy

使用相干拉曼显微光谱检测局部纳米环境

• 批准号: 10477258
• 负责人: Vladislav V. Yakovlev
• 金额: $ 21.82万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10477258
• 关键词: Affect; Biochemical Reaction; Biological Process; Cell physiology; Cells; Chemicals; Concentration measurement; Detection; Environment; Goals; Heterogeneity; Image; In Vitro; Label; Life; Lipids; Measures; Methods; Microscope; Microscopic; Microscopy; Modality; Molecular; Monitor; Phase; Photobleaching; Physiologic pulse; Raman Spectrum Analysis; Relaxation; Research; Research Proposals; Resolution; Sensitivity and Specificity; Signal Transduction; Speed; Structure; System; Time; Work; contrast imaging; detection sensitivity; experimental study; imaging modality; improved; in vivo; live cell imaging; microscopic imaging; molecular imaging; multiplexed imaging; nanoenvironment; novel; novel strategies; optical imaging; pilot test; programs; prototype; response; single molecule; spectroscopic imaging; temporal measurement; tool; vibration

ABSTRACT This exploratory research proposal which aims to investigate a novel approach for interrogating local nano- environments within a living cell by improving the detection sensitivity of coherent Raman microspectroscopy as applied to live-cell imaging. The proposed new approach overcomes the existing shortcomings of coherent Raman spectroscopy. It provides an exquisite tool of microscopic chemically specific imaging with greatly improved sensitivity of detection and exceptional sensitivity to the local nano-environment. The central hypothesis is that vibrational coherence excited by two broadband pulses is sensitive to the local environment and can be precisely measured by interrogating this coherence using a third pulse at a variable time delay. By choosing a molecular vibration with a relatively narrow spectral line that corresponds to a long relaxation time, and measuring time-delayed response, background signal can be substantially reduced at long delay times of the probe pulse. This adjustment makes low-concentration measurements possible. The overall research goal of the proposed program is to validate this hypothesis by demonstrating sensitivity of coherent Raman microspectroscopy to a local nano-environment and by improving the detection sensitivity of Raman spectroscopy. The specific aims of the proposed activities are focused on constructing a prototype of a microscope and exploring its capabilities using both in vitro and in vivo systems. The proposed research program is expected to deliver a novel and label-free optical imaging modality with enhanced sensitivity and specificity that is suitable for live-cell imaging.

抽象的 这项探索性研究建议旨在研究一种新型方法，以询问当地纳米纳米 - 通过提高相干拉曼微光谱的检测灵敏度，活细胞内的环境 适用于活细胞成像。提出的新方法克服了连贯的现有缺点 拉曼光谱法。它提供了一种精美的微观化学特定成像工具 提高了检测的灵敏度和对局部纳米环境的敏感性。中央 假设是，通过两个宽带脉冲激发的振动连贯性对局部环境敏感 并且可以通过在可变的时间延迟下使用第三个脉冲询问这种连贯性来精确衡量。经过 选择具有相对狭窄的光谱线的分子振动，该光谱线对应于较长的松弛时间 并测量时间延迟的响应，可以在长时间延迟时间大大减少背景信号 探针脉冲。这种调整使低浓度的测量成为可能。总体研究目标 拟议的计划的是通过证明相干拉曼的敏感性来验证这一假设 微光谱对局部纳米环境，并通过提高拉曼的检测灵敏度 光谱法。拟议活动的具体目的是构建一个原型 显微镜并使用体外和体内系统探索其功能。拟议的研究 预计计划将提供一种新颖且无标签的光学成像方式，并具有增强的灵敏度和 适合活细胞成像的特异性。

项目成果

Spatial multiplexing for robust optical vortex transmission with optical nonlinearity.

空间复用可实现具有光学非线性的鲁棒光学涡旋传输。

• DOI: 10.1364/oe.500765
• 发表时间: 2023
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Fan,Weiru;Chen,Tianrun;Tang,Xiaobin;Xu,Xingqi;Yuan,Luqi;Yakovlev,VladislavV;Zhu,Shi-Yao;Wang,Da-Wei;Zhang,Delong
• 通讯作者: Zhang,Delong