Cellular Systems Core

蜂窝系统核心

• 批准号: 10454989
• 负责人: JAMES A. LEDERER
• 金额: $ 26.71万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-11 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10454989
• 关键词: ATAC-seq; Adoption; Animals; Antibodies; Antigens; Area; Arthritis; Autoantigens; B cell repertoire; B-cell receptor repertoire sequencing; Basic Science; Big Data; Bioinformatics; Biology; Boston; CRISPR screen; Categories; Cells; Cellular Indexing of Transcriptomes and Epitopes by Sequencing; Clustered Regularly Interspaced Short Palindromic Repeats; Communities; Complex; Computational Biology; Computer Analysis; Computing Methodologies; Custom; Cytometry; DNA sequencing; Data; Data Analyses; Data Science; Data Set; Detection; Development; Disease; Education; Ensure; Experimental Designs; Face; Genomic DNA; Genomics; Goals; Hospitals; Human; Immunology; Immunophenotyping; Individual; Infrastructure; Institutes; Investments; Joints; Laboratories; Lead; Liquid substance; Logistics; Lupus; Mass Spectrum Analysis; Medical; Medicine; Methodology; Methods; Mission; Pathologist; Pediatric Hospitals; Phage ImmunoPrecipitation Sequencing; Preparation; Proteins; Research; Research Personnel; Resources; Rheumatoid Arthritis; Rheumatology; Sampling; Scanning; Services; System; Systemic Lupus Erythematosus; T-Lymphocyte; Techniques; Technology; Testing; Time; Tissues; Translational Research; Woman; antibody libraries; assay development; base; cell dimension; design; high dimensionality; innovation; member; next generation sequencing; programs; proteogenomics; rheumatologist; service delivery; single-cell RNA sequencing; structured data; success; tissue preparation; tissue processing; tool; transcriptome sequencing; transcriptomics; translational scientist

PROJECT SUMMARY/ABSTRACT – Resource Core 2: Cellular Systems Core Investigators in the Joint Biology Consortium (JBC) share a set of challenges that impede utilization of state-of- the-art technologies for research in arthritis and related diseases. Building on success in the first cycle of the JBC, the Cellular Systems Core (CSC) will leverage local resources and economies of scale to cultivate an integrated set of research tools to serve JBC members. The CSC will provide two broad categories of service. First, the CSC will deliver technologies realized through next-generation sequencing. These include single-cell RNAseq, ATAC-seq, T- and B-cell receptor sequencing, genomic DNA sequencing, spatial transcriptomics, CRISPR-based genomic perturbation screens, and autoantigen detection through PhIP-seq and T-scan. Second, the CSC will deliver tools centered on protein detection. These include optimized cytometry by time of flight (CyTOF), tissue mass cytometry, and Luminex-based screens for economical, customizable detection of cyctokines, antibodies, and other proteins. Resulting data will be analyzed through the new Joint Biology Consortium Bioinformatics Core, providing both high-end big-data analysis and structured education and coaching for JBC members, with the goal of developing a community of investigators conversant with advanced computational methods. This program integrates services available through the Brigham and Women’s Hospital Single-Cell Genomics Core and Center for Data Sciences, the Harvard Medical Area CyTOF Core, the Boston Children’s Hospital Division of Immunology, the Broad Institute’s Genomic Perturbation Platform, and other affiliated cores and labs. Progressive optimization of CSC services – now encompassing both cells and intact tissues – will result further through close affiliation with technical and computational leaders of the Accelerating Medicines Partnership (AMP) consortium in rheumatoid arthritis and lupus, ensuring JBC members access to the latest methodologic advances. Service delivery will be led by a scientific team consisting of Harvard CyTOF director Dr. James Lederer supported by associate directors Dr. Kevin Wei and bioinformatician Dr. Maria Gutierrez- Arcelus. This team will ensure that the logistical investment represented by the Cellular Systems Core continues to accelerate innovative translational arthritis research within the JBC.

项目摘要/摘要 - 资源核心2：蜂窝系统核心 联合生物联盟（JBC）的研究人员分享了一系列挑战 关节炎和相关疾病研究的艺术技术。基于成功的基础 JBC，蜂窝系统核心（CSC）将利用当地资源和规模经济来培养 为JBC成员提供服务的集成研究工具集。 CSC将提供两个广泛的服务类别。 首先，CSC将提供通过下一代测序实现的技术。这些包括单细胞 RNASEQ，ATAC-SEQ，T-和B细胞受体测序，基因组DNA测序，空间转录组学， 基于CRISPR的基因组扰动筛选以及通过PHIP-SEQ和T-SCAN的自身抗原检测。 其次，CSC将提供以蛋白质检测为中心的工具。这些包括按时间的优化细胞仪 飞行（细胞），组织质量细胞仪和基于Luminex的筛选，用于经济，可定制的检测 细胞因子，抗体和其他蛋白质。 最终的数据将通过新的联合生物学联盟生物信息学核心进行分析，从而提供两者 高端大数据分析以及JBC成员的结构化教育和教练，目的是 开发一个具有先进计算方法的调查人员convert依者社区。 该计划集成了通过杨百翰和妇女医院单细胞基因组学的服务 核心和数据科学中心，哈佛医疗区Cytof Core，波士顿儿童医院 免疫学部，广泛研究所的基因组扰动平台以及其他会员核心 实验室。 CSC服务的进行性优化 - 现在涵盖了细胞和完整的组织 - 将导致 进一步通过与加速药物的技术和计算领导者密切相关 类风湿关节炎和狼疮的伙伴关系（AMP）财团，确保JBC成员访问最新 方法学进步。服务交付将由一个科学团队领导，由哈佛大学董事组成 詹姆斯·莱德勒（James Lederer）博士在副董事Kevin Wei博士和生物信息学家Maria Gutierrez-- Arcelus。该团队将确保由蜂窝系统核心代表的后勤投资 继续加速JBC内的创新转化关节炎研究。