Light Sheet Microscopy for Einstein Shared Facility

爱因斯坦共享设施的光片显微镜

• 批准号: 10440903
• 负责人: KOSTANTIN DOBRENIS
• 金额: $ 57.27万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10440903
• 关键词: Atherosclerosis; Behavioral Sciences; Biomedical Research; Cells; Cognition; Communities; Complex; Development; Epilepsy; Evaluation; Financial Support; Funding; Grant; Image; Infanticide; Intellectual and Developmental Disabilities Research Centers; Label; Lead; Light; Medicine; Memory; Microscope; Microscopy; National Institute of Child Health and Human Development; Neurodevelopmental Disorder; Neuronal Plasticity; Neurons; Neurosciences; Obesity; Organ; Pathway interactions; Pattern; Population; Productivity; Psychiatry; Research Personnel; Research Project Grants; Schools; Stress; Technology; Time; Tissues; United States National Institutes of Health; Vascular remodeling; addiction; biological research; cell type; cellular engineering; cellular imaging; college; imaging approach; insight; novel

This proposal seeks funding to purchase the Zeiss Light Sheet 7 microscope for the Neural Cell Engineering and Imaging Core (NCEI) of the Rose F Kennedy Center for Intellectual and Developmental Disabilities Research (IDDR) at the Albert Einstein College of Medicine (Einstein). The NCEI Core has served as a valuable shared facility for Einstein investigators for close to 35 years. During this the Core has received strong financial support from the Kennedy IDDR Center, funded by NIH/NICHD Center grants, the Department of Neuroscience, and the school. In recent years there has been marked increase in development and use of light sheet imaging approaches to biological and biomedical research, facilitating comprehensive evaluations not otherwise easily obtained. This provides unprecedented global perspective to tissue patterning, cellular architectonics, and functional ensembles and long-range connectivity, and can lead to unanticipated new insights. Establishment of a light sheet microscope facility will bring this important technology to the Einstein community for the first time, and meet what has been understandably a significantly growing demand. The ability to rapidly image large intact volumes of tissues and organs will be invaluable to numerous NIH-funded investigators of the IDDR center and of the Neuroscience, Medicine, Psychiatry and Behavioral Sciences departments, as well as others. In all cases, the microscope will be transformational in their studies that call for examination of long distance neuronal pathways, volume determinations, labeled specific cell sub-population counts and distributions, and complex mixed cell-type arrays. The research projects have relevance to neurodevelopmental disorders, cognition, addiction, neural plasticity, epilepsy, infanticide, atherosclerosis and vascular remodeling, obesity, and stress and memory. The capabilities provided by the proposed microscope would be a vital asset for Einstein researchers, permitting studies not otherwise readily possible, enhancing productivity and potentiating novel biomedical insights.

该提案寻求资金购买神经细胞的Zeiss Light纸7显微镜 罗斯·肯尼迪智力中心的工程和成像核心（NCEI）和 爱因斯坦医学院（爱因斯坦）的发展障碍研究（IDDR）。 NCEI核心已成为爱因斯坦调查人员的宝贵共享设施，可接近35 年。在此期间，核心得到了肯尼迪IDDR中心的大力财政支持， 由NIH/NICHD中心赠款，神经科学系和学校资助。最近 几年的开发和使用轻度成像方法的增长明显增加 生物学和生物医学研究，促进综合评估并不容易 获得。这为组织模式，细胞提供了前所未有的全球视角 建筑学，功能合奏和远程连接，并可能导致意外 新见解。建立轻型显微镜设施将使这项重要技术 爱因斯坦社区第一次，并满足已经很重要的 需求不断增长。快速成像大量完整的组织和器官的能力将是 对于IDDR中心和神经科学的众多NIH资助的调查员来说，无价 医学，精神病学和行为科学部门以及其他部门。在所有情况下， 显微镜将在他们的研究中进行转化，该研究需要检查长距离神经元 途径，确定音量，标记为特定的细胞亚构件计数和分布，以及 复杂的混合细胞型阵列。研究项目与神经发育有关 疾病，认知，成瘾，神经可塑性，癫痫，杀婴，动脉粥样硬化和血管 重塑，肥胖以及压力和记忆。拟议的显微镜提供的功能 对于爱因斯坦研究人员而言，这将是重要的资产，允许不容易研究研究， 提高生产力并增强新型生物医学见解。