NEURAL CELL ENGINEERING AND IMAGING CORE

神经细胞工程和成像核心

基本信息

批准号：
10669070
负责人：
KOSTANTIN DOBRENIS
金额：
$ 18.94万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-23 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10669070
关键词：
Address Anatomy Animal Experiments Animals Back Biological Assay Brain Cell Line Cell Separation Cell model Cells Cognitive Computer Workstations Computer software Consultations Correlation Studies Coupled Custom Data Analyses Disease Dissection Electrophysiology (science)Embryo Epigenetic Process Equipment Evaluation Experimental Designs Faculty Funding Gene Expression Gene Targeting Generations Genetic Goals Grant Human Image Image Analysis In Situ In Vitro Individual Injections Intellectual and Developmental Disabilities Research Centers Intellectual functioning disability Investigation Investments Laboratories Lasers Leadership Microscope Microscopy Modernization Modification Molecular Monitor Mutation Names Neuronal Differentiation Neurons Neurosciences Organoids Patients Phenotype Physiological Preparation Printing Production Productivity Proteins Proteomics Protocols documentation Publications Quality Control RNA Research Research Personnel Research Project Grants Resolution Resource Sharing Resources Sampling Schedule Services Slice Specimen Structure Surveys Therapeutic Intervention Time Tissue Engineering Tissue Model Tissues Training United States National Institutes of Health Vertebral column Work base behavioral phenotyping brain cell cell preparation cellular engineering cellular imaging clinical phenotype college computerized data processing data acquisition design differentiation protocol fetus cell gene therapy high end computer high standard image processing image reconstruction imaging software in vivo induced pluripotent stem cell innovation instrument interest mouse model neural neurogenomics neuroimaging optogenetics reconstruction synergism therapeutic evaluation tissue preparation tissue/cell culture tool translational study treatment strategy ultra high resolution

项目摘要

PROJECT SUMMARY/ABSTRACT – (Core D: NCEI Core) The Neural Cell Engineering and Imaging Core (Core D, NCEI) has been a vital component of the Kennedy Center for 30 years, steadily growing in resources and user base, with 70 individuals from 50 IDDRC laboratories utilizing the Core in the last 3 years alone. The NCEI Core consists of 3 major divisions: The Cell and Tissue Engineering Subcore, with facilities and expertise for cell/tissue culture work including primary, embryonic, iPS cells and modern neural differentiation protocols. The Modern Microscopy Subcore, with instruments for tissue sectioning and imaging. And the Analysis and Graphics Subcore, which provides workstations with sophisticated software for deconvolution, quantitative 2D to 5D image analyses and reconstruction, custom software and macros, as well as graphics and printing services. To optimize use of these tools for IDDRC investigators and their trainees, NCEI leadership provides in-depth user consultation, emphasizes well-planned experimental design, and engages in vigilant monitoring of project progress. The Core’s value is extended linkage with expert advisors allowing studies on gene targeting, gene therapy, RNA constructs, optogenetics, electrophysiology, and human ES, iPS and fetal cells, including organoids. Productivity and innovation are also enhanced by interaction with other IDDRC Cores, coordinating, e.g., studies correlating brain cell and anatomic changes with behavioral phenotypes (AP), genetic or epigenetic evaluations on isolated cell subpopulations/single cells (NGEN), and translational studies on reprogrammed cells and iPSC-derived organoids from patients (HCP). Core resources also serve the P50 research project on KDM5C disease by providing imaging and quantitative analyses of changes in gene expression, analysis of neural subtypes and dendritic changes in mouse models, and generating iPS cells/organoids and differentiating neurons from patient samples carrying KDM5C mutations. The Core maintains high standards of quality control through scheduled internal evaluations and the annual user survey carried out by the ADM Core. Collectively, the NCEI Core represents a significant part of the scientific bridge between genetics and cognitive phenotypes and as such has a broad and significant impact on the research carried out by IDDRC investigators and their trainees focused on, and critical to, understanding and treating IDDs.

项目摘要/摘要 –（核心 D：NCEI 核心）神经细胞工程和成像核心（Core D，NCEI）一直是肯尼迪实验室的重要组成部分中心成立 30 年，资源和用户群稳步增长，拥有来自 50 个 IDDRC 的 70 名人员仅在过去 3 年就使用该核心的实验室 NCEI 核心由 3 个主要部门组成：单元。和组织工程子核心，拥有细胞/组织培养工作的设施和专业知识，包括初级、胚胎、iPS 细胞和现代神经分化方案，以及现代显微镜子核心。用于组织切片和成像的仪器以及分析和图形子核心，它提供了配备先进软件的工作站，用于反卷积、定量 2D 至 5D 图像分析和重建、定制软件和宏，以及图形和打印服务以优化使用。这些工具为 IDDRC 调查员及其受训人员、NCEI 领导层提供深入的用户咨询、强调精心策划的实验设计，并对项目进展进行密切监控。 Core 的价值在于与专家顾问的扩展联系，允许对基因靶向、基因治疗、RNA 进行研究构建体、光遗传学、电生理学以及人类 ES、iPS 和胎儿细胞，包括类器官。与其他 IDDRC 核心的交互、协调也可以提高生产力和创新，例如将脑细胞和解剖学变化与行为表型 (AP)、遗传或表观遗传相关的研究对分离细胞亚群/单细胞（NGEN）的评估以及重编程的转化研究来自患者的细胞和 iPSC 衍生的类器官 (HCP) 核心资源也服务于 P50 研究项目。 KDM5C 疾病通过提供基因表达变化的成像和定量分析，分析小鼠模型中的神经亚型和树突状变化，并生成 iPS 细胞/类器官和从携带 KDM5C 突变的患者样本中区分神经元，Core 保持了高标准。通过定期内部评估和 ADM 进行的年度用户调查进行质量控制总体而言，NCEI 核心代表了遗传学和遗传学之间科学桥梁的重要组成部分。认知表型，因此对 IDDRC 进行的研究具有广泛而重大的影响研究人员及其受训者重点关注理解和治疗 IDD，这对理解和治疗 IDD 至关重要。