Dissemination of MAPseq and BARseq for high-throughput brain mapping

传播用于高通量脑图谱的 MAPseq 和 BARseq

基本信息

批准号：
10440138
负责人：
ANTHONY M ZADOR
金额：
$ 109.59万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2027-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10440138
关键词：
Age Anatomy Animal Model Animals Area Atlases Axon Bar Codes Biological Models Brain Brain Mapping Brain region Carrier Proteins Collaborations Communities Complex Computer software Custom DNA sequencing Data Development Distant Engineering Foundations Gender Gene Expression Generations Genes Genetic Models Gilles de la Tourette syndrome Goals High-Throughput DNA Sequencing In Situ Infection Interneurons Label Laboratories Libraries Maps Mental Depression Methods Multiplexed Analysis of Projections by Sequencing Mus Neocortex Neuroanatomy Neurofibrillary Tangles Neurons Neuropil Neurosciences Neurosciences Research Population Presynaptic Terminals Probability Protocols documentation Publications Publishing RNA Reagent Resolution Reverse Transcription Role Schizophrenia Scientist Specificity Standardization Synapses Techniques Technology Time Training and Education Viral Virus Vision Work addiction autism spectrum disorder base bioinformatics pipeline brain tissue cost in situ sequencing insight interest neuronal circuitry neuropsychiatric disorder next generation next generation sequencing novel novel strategies relating to nervous system skills tissue processing transcriptomics user-friendly

项目摘要

The goal of this project is to disseminate MAPseq and BARseq to the broader neuroscience community. These are novel methods developed in my laboratory based on high-throughput DNA sequencing for determining neuronal circuitry. Neurons transmit information to distant brain regions via long-range axonal projections. In some cases, functionally distinct populations of neurons are intermingled within a small region. Disruptions of connectivity may underlie many neuropsychiatric disorders including autism and schizophrenia. At present, neuroanatomical techniques—particularly those with single neuron resolution—are expensive and labor intensive. MAPseq and BARseq convert neuroanatomy into a problem of DNA sequencing, and thereby allow us to exploit the tremendous breakthroughs in next- generation sequencing throughput. The dissemination of these high-throughput methods for determining neuronal projections will have important implications for understanding normal neuronal circuitry, and how this circuitry is disrupted in animal models of neuropsychiatric disorders like autism and schizophrenia.

该项目的目的是将MapSeq和Barseq传播到更广泛的神经科学社区。这些是基于高通量DNA在我的实验室中开发的新方法确定神经元电路的测序。神经元将信息传输到遥远的大脑通过远程轴突预测区域。在某些情况下，功能上不同的人群神经元在一个小区域中混合在一起。连通性的中断可能是许多的基础神经精神疾病，包括自闭症和精神分裂症。目前，神经解剖技术（尤其是具有单个神经元分辨率的技术）是昂贵且劳动密集型。 MapSeq和Barseq将神经解剖学转化为一个问题 DNA测序，从而使我们能够利用接下来的巨大突破生成测序吞吐量。这些高通量方法的传播确定神经元预测将对理解正常有重要意义神经元电路，以及该电路如何破坏神经精神病学的动物模型自闭症和精神分裂症等疾病。