Cell-type-specific control of information flow between brain regions

大脑区域之间信息流的细胞类型特异性控制

• 批准号: 10294677
• 负责人: ANTHONY M ZADOR
• 金额: $ 64.85万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10294677
• 关键词: Anatomy; Animals; Area; Auditory area; Axon; Bar Codes; Behavioral; Brain; Brain region; Cells; Communication; Complex; DNA; Data; Data Analyses; Decision Making; Evaluation; Gene Expression; Gene Expression Profiling; Genes; Genetic Markers; Genetic Transcription; Goals; Image; In Vitro; Individual; International; Knowledge; Laboratories; Light; Measures; Mediating; Methods; Modeling; Mus; Neurons; Pattern; Physiological; Property; Resolution; Structure; Synaptic Transmission; Techniques; Testing; Tissues; Work; anatomical tracing; base; cell type; data management; design; experimental study; in vivo; in vivo calcium imaging; in vivo two-photon imaging; insight; member; multi-electrode arrays; neural circuit; novel; relating to nervous system; transcriptome; transcriptomics; two-photon

Summary/Abstract, Project 5 The goal of this project is to comprehensively examine, at single-neuron resolution, the flow of information through the brain during a decision-making task. Leveraging the expertise, knowledge, and data gained from International Brain Laboratory members who have designed and studied this task, the proposed approach combines novel, advanced techniques for high- throughput, high-resolution physiological recordings and anatomical tracing to determine the substrates of inter-regional communication. In light of the extensive functional and structural diversity of neurons in the brain (for example, neurons in the auditory cortex can project to at least 11 different targets, forming 264 distinct projection patterns), the proposed experiments test the central hypothesis that specific privileged subpopulations mediate the flow of information between regions in a state-dependent manner. Such subpopulations will be identified based on transcriptionally defined cell subtypes (Aim 1) and projection types or motifs (Aim 2). In vivo two- photon calcium imaging will be combined with in vitro profiling of transcriptional and anatomical identity via the novel barcoded anatomy resolved by sequencing (BARseq) technique. BARseq is used to identify the projections and/or genetic markers of thousands or tens of thousands of neurons simultaneously in a single animal. Because the two techniques are both based on imaging, the in vivo data will be registered with in vitro data to support structure-function, state- dependent insights at the single-neuron level. The regions and circuits targeted in these experiments will be informed by the results of Projects 1 and 2. In turn, the results generated here in Project 5 will inform the modeling work proposed in Project 3.

摘要/摘要，项目5 该项目的目的是通过单神经元分辨率进行全面检查 在决策任务中通过大脑信息信息。利用专业知识， 知识以及从设计的国际脑实验室成员获得的数据 并研究了这项任务，提出的方法结合了新颖的高级技术 吞吐量，高分辨率的生理记录和解剖学追踪，以确定 区域间通信的基板。鉴于广泛的功能和结构 大脑中神经元的多样性（例如，听觉皮层中的神经元至少可以投射到 11个不同的目标，形成264个不同的投影模式），提出的实验测试了 中心假设，即特定特权亚群介导了信息流 以国家依赖的方式进行区域。将根据 转录定义的细胞亚型（AIM 1）和投影类型或基序（AIM 2）。 invivo二 光子钙成像将与转录和解剖学的体外分析结合 通过测序（BARSEQ）技术解决的新型条形码解剖结构的身份。 Barseq是 用于识别成千上万或数万的投影和/或遗传标记 神经元同时在单个动物中。因为这两种技术都是基于 成像，体内数据将在体外数据中注册，以支持结构功能，状态 - 单神经元级别的依赖见解。这些针对的区域和电路 实验将由项目1和2的结果告知。反过来，此处产生的结果 在项目5中，将告知项目3中提出的建模工作。