National Gnotobiotic Rodent Resource Center

国家知生啮齿动物资源中心

基本信息

批准号：
10432013
负责人：
Ryan B Sartor
金额：
$ 54.17万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10432013
关键词：
Address Animals Applications Grants Area Atherosclerosis Bacteria Bacterial Genes Behavioral Breeding Cells Chronic Disease Colon Complex Custom Data Derivation procedure Diabetes Mellitus Diet Disease Embryo Transfer Environmental Risk Factor Etiology Film Funding Funding Agency Genes Genetic Genetic Engineering Genetic Predisposition to Disease Genetic Risk Genetically Engineered Mouse Germ-Free Gnotobiotic Grant Housing Human Human Microbiome Human Resources Inbred Mouse Individual Infection Inflammatory Institutes Institution Intramural Research Program Knock-out Libraries Link Malignant neoplasm of pancreas Mental Depression Metabolic Metabolic syndrome Microbe Modeling Monitor Mouse Strains Mus Mutant Strains Mice Mutate National Institute of Allergy and Infectious Disease Nature Obesity Organ Paper Parasites Pathogenicity Peer Review Physiological Physiological Processes Physiology Pilot Projects Principal Investigator Publishing Quality Control Reproducibility of Results Research Research Personnel Resources Retroviridae Rodent Role Series Sterility Technical Expertise Techniques Tissue Banks Tissues Training Training Support Transgenic Organisms Transplantation United States National Institutes of Health Universities Viral Virus Yeasts Zebrafish autism spectrum disorder bacterial community base deep sequencing design dietary disease phenotype dysbiosis experience fecal transplantation flexibility germ free condition host-microbe interactions human disease improved interest metabolomics microbial microbial community microbial host microbiota microorganism interaction mouse model multidisciplinary neoplastic nonalcoholic steatohepatitis response

项目摘要

Project Summary: Environmental factors, especially microbiota, modify genetic susceptibility to many chronic diseases. The NGRRC provides an essential resource for local, regional and national multidisciplinary investigators to explore the hypothesis that residental bacteria strongly influence physiologic processes in normal hosts and pathogenic inflammatory, metabolic and neoplastic responses in genetically susceptible hosts. This unit provides a resource for broadly based NIH-funded investigators to examine physiologic and pathophysiologic differences in germ-free (GF, sterile) vs. gnotobiotic (selectively colonized) vs. specific pathogen free mice of different genetic backgrounds, to explore the functional alterations of normal vs. dysbiotic microbiota in murine models and human diseases, and to define the functional relevance of bacterial genes. The microbiota can be precisely manipulated by colonizing GF mice with single or multiple resident or pathogenic bacterial, viral or fungal strains using isogenic wild type or mutated strains. In addition, fecal transplants can be performed from murine models or human donors. Aims: 1. Provide GF and gnotobiotic WT and mutant mice, their tissues and cells to NIH-funded investigators. 2. Derive additional GF mouse strains for NIH -funded investigators. 3. Support pilot studies for new investigators to generate preliminary data for NIH grant applications. 4. Train personnel to develop murine and zebrafish gnotobiotic facilities in other institutions. 5. Develop and characterize complex human and murine microbial communities and a library of bacteria strains to facilitate reproducible results in gnotobiotic studies (Research Component). We provide a unique and essential resource for a multidisciplinary group of NIH-funded investigators to study physiologic and patho-physiologic functions of normal and dysbiotic resident bacteria, with particular emphasis on gene/environ-mental interactions in genetically altered gnotobiotic mice (transgenic, knockout or spontaneously mutated) with altered physiology and disease phenotypes. In the past 5 years of funding, the NGRRC provided 8279 gnotobiotic mice to 101 investigators in 49 institutions. 68 funded NIH grants (in 12 Institutes) by 51 investigators as well as 63 grants by other funding agencies depend on gnotobiotic mice from the NGRRC.

项目概要：环境因素，尤其是微生物群，会改变许多慢性疾病的遗传易感性。这 NGRRC 为地方、区域和国家多学科研究人员探索提供重要资源常驻细菌强烈影响正常宿主的生理过程的假设遗传易感宿主的致病性炎症、代谢和肿瘤反应。本单位为 NIH 资助的研究人员提供广泛的资源来检查生理学和病理生理学无菌（GF，无菌）小鼠与无菌小鼠（选择性定植）与无特定病原体小鼠的差异不同的遗传背景，探索小鼠正常微生物群与失调微生物群的功能变化模型和人类疾病，并定义细菌基因的功能相关性。微生物群可以是通过用单个或多个常驻或致病细菌、病毒或细菌定植 GF 小鼠来精确操纵使用等基因野生型或突变菌株的真菌菌株。此外，还可以进行粪便移植小鼠模型或人类捐赠者。目的： 1. 提供GF和限生WT及突变小鼠及其组织和细胞交给 NIH 资助的研究人员。 2. 为 NIH 资助的研究人员获得额外的 GF 小鼠品系。 3. 支持新研究人员的试点研究，为 NIH 拨款申请生成初步数据。 4. 火车人员在其他机构开发小鼠和斑马鱼的生殖设施。 5. 开发和描述复杂的人类和小鼠微生物群落和细菌菌株库，以促进限菌研究中可重复的结果（研究部分）。我们提供独特且必要的 NIH 资助的多学科研究小组研究生理学和病理生理学的资源正常和失调的常驻细菌的功能，特别强调基因/环境基因改造的限生小鼠（转基因、敲除或自发突变）与生理学和疾病表型改变。在过去5年的资助中，NGRRC提供了8279 向 49 个机构的 101 位研究人员提供了限生小鼠。 68 项 NIH 拨款（在 12 个研究所）由 51 人资助研究人员以及其他资助机构的 63 项资助都依赖于 NGRRC 的限生小鼠。