Cleared Tissue Large FOV Microscope Request

透明组织大视场显微镜请求

• 批准号: 10429884
• 负责人: Adam I. Marcus
• 金额: $ 30.84万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429884
• 关键词: 3-Dimensional; 3D Print; Address; Brain; Case Study; Cells; Cerebral hemisphere; Communities; Core Facility; Development; Disease; Ensure; Equilibrium; Fluorescence; Funding; Goals; Grant; Heart Diseases; Hormones; Human; Image; Imaging technology; Institutes; Institution; Intestines; Intrahepatic bile duct; Investigation; Length; Light; Macular degeneration; Magnetic Resonance Imaging; Malignant Neoplasms; Metastatic Neoplasm to the Liver; Microscope; Microscopy; Molecular; Mus; Nature; Neoplasm Metastasis; Neurologic; Oncology; Ophthalmology; Pathology; Policies; RNA; Request for Applications; Research; Research Personnel; Resolution; Resource Sharing; Sampling; Services; Site; Specificity; Speed; Structure of retinal pigment epithelium; System; Techniques; Technology; Tissues; United States National Institutes of Health; Universities; Yang; base; biofabrication; cellular imaging; clinical application; flexibility; health science research; interest; liver injury; medical schools; neurosensory; novel; tool; transcriptomics; tumor microenvironment

This application requests funds to purchase a Miltenyi UltraMicroscope Blaze fluorescence light sheet microscope, to be included in the existing Integrated Cellular Imaging Core (ICIC). The primary goal of the ICIC is to provide investigators at Emory and surrounding Georgia institutions access to cutting edge light-based microscope technology, services, and expertise. This open shared resource has assisted 207 NIH grants held by 142 investigators in the last 5 FYs. Within the facility, the UltraMicroscope Blaze will be used to study an open and broad range of applications that bridge the gap from molecular level fluorescence specificity to systems level distributions and connectivity. Tissue clearing and light sheet imaging technology provide a high 3D resolution (~1 µm) across samples as large as 10 cm. From molecular level hormone distributions in neurological studies that span regions of an entire spiny mouse brain hemisphere (Kelly), to metastasis sites and their corresponding tumor microenvironments in whole mouse oncology (Lesinski), this relatively new microscopy technique provides a more complete picture than ever before. Further applications include 3D printing and bio fabrication investigations with direct clinical application for heart disease and brain development (Serpooshan), ophthalmological studies of retinal pigment epithelium cells and neurosensory connectivity in macular degeneration (Nickerson), 3D remodeling of intrahepatic bile ducts in liver injury (Gracz), and a combination of 3D pathology with single cell RNA transcriptomics in human intestine (Kugathasan). These case studies represent a small subset of the vast interest on Emory campus, and highlight the diversity and collaborative nature between institutions. An Emory/Georgia State University collaborating case study truly exemplifies the broad impact attainable from length scales encompassed by this light sheet microscope, detailing how novel molecular level metastatic regulators can be utilized in both MRI and fluorescence light sheet imaging, providing a unique, comprehensive, and longitudinal view of the key determinants of liver metastasis (Yang, GSU). The Miltenyi Blaze combines a unique balance of ease of use, flexibility, speed, together with the largest viable samples, thus providing researchers with expedited answers to their disease related longitudinal questions. This microscope will be managed as part of the facilities under the same stringent policies to ensure optimal usage and minimize downtime. The shared resources at Emory have a strong commitment marked by the largest set of core facility suites on Emory campus, housed in the soon to be completed 2nd Health Science Research Building (HSRB II), and the ICIC has a strong financial commitment from the School of Medicine and Winship Cancer Institute, including deficit coverage and supporting purchase funds. We feel the Miltenyi Blaze is an essential tool for addressing both current and future research needs of a largely NIH funded research community at Emory and surrounding institutions.

该申请要求资金购买Miltenyi Ultramicroscope Blaze荧光灯板 显微镜，将包括在现有的集成细胞成像核心（ICIC）中。 ICIC的主要目标 是为埃默里（Emory）和周围佐治亚州机构的调查人员提供访问尖端的基于光线 显微镜技术，服务和专业知识。这种开放的共享资源已协助持有的207个NIH赠款 在最后5个Fys中，有142位调查人员。在设施中，超显微镜大火将用于研究 开放和广泛的应用，这些应用弥合了从分子水平荧光特异性到系统的差距 级别分布和连接性。组织清除和轻板成像技术提供了高3D 跨样品的分辨率（〜1 µm）至10 cm。来自神经学中的分子水平骑马分布 跨越整个多刺小鼠脑半球区域（凯利）的研究，到转移部位及其 整个小鼠肿瘤学（Lesinski）中相应的肿瘤微环境，这种相对较新的显微镜 技术比以往任何时候都更完整。进一步的申请包括3D打印和生物 对心脏病和脑发育直接临床应用（Serpooshan）的制造研究， 视网膜色素上皮细胞和黄斑神经连通性的眼科研究 变性（尼克森），肝损伤（Gracz）中epatitic胆管的3D重塑和组合 人类肠道（Kugathasan）中具有单细胞RNA转录组学的3D病理学。这些案例研究 代表了埃默里校园的巨大兴趣的一小部分，并强调了多样性和协作 机构之间的性质。埃默里（Emory）/佐治亚州立大学合作的案例研究真正地说明了 从该轻度显微镜包含的长度尺度上可以实现的广泛影响，详细介绍了新颖的方式 分子水平转移调节剂可以在MRI和荧光光片成像中使用，提供 肝转移的关键决定者（Yang，GSU）的独特，全面和纵向的观点。 Miltenyi Blaze结合了易用性，灵活性，速度的独特平衡，以及最大的可行 样本，从而为研究人员提供了与疾病相关的纵向问题的加快答案。这 显微镜将作为设施的一部分进行管理，以确保最佳用法 并最大程度地减少停机时间。埃默里（Emory）的共享资源具有最大的集合标志 Emory校园的核心设施套件，该套房即将完成第二次健康科学研究 建筑物（HSRB II），ICIC具有医学和胜利学院的强烈财务承诺 癌症研究所，包括赤字覆盖范围和支持购买资金。我们觉得Miltenyi大火是 满足NIH资助的研究社区的当前和未来研究需求的重要工具 在埃默里（Emory）和周边机构。