Human Tissue and Sample Acquisition

人体组织和样本采集

• 批准号: 10426133
• 负责人: Donna L. Farber
• 金额: $ 33.92万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-25 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426133
• 关键词: 3-Dimensional; Academic Medical Centers; Area; Atlases; B-Lymphocytes; Biopsy; Biopsy Specimen; Blood specimen; Bone Marrow; Cells; Clinical Management; Collaborations; Computational algorithm; Consent; Enrollment; Goals; Human; Human Resources; Image; Immune; Immune response; Immune system; Immunology; Infrastructure; Institutional Review Boards; Intestines; Intramural Research; Laboratories; Large Intestine; Life; Lung; Lymphocyte; Lymphoid; Lymphoid Tissue; Methods; Molecular; Mucous Membrane; National Institute of Allergy and Infectious Disease; Organ Donor; Organ Procurements; Organ Transplantation; Patients; Peripheral; Population; Process; Protocols documentation; Research; Research Personnel; Sampling; Savings; Services; Site; Small Intestines; Source; Spleen; Surgeon; Suspensions; System; T cell reconstitution; T memory cell; Thymus Gland; Tissue Preservation; Tissue Sample; Tissue imaging; Tissues; Tonsil; Transplant Recipients; Transplantation; Work; biobank; draining lymph node; gastrointestinal transplantation; high dimensionality; human tissue; metropolitan; novel; peripheral blood; programs; repository; tissue resource

Core B: Project Summary The main goal of this program is to conduct a novel analysis of lymphocyte populations in diverse human lymphoid and mucosal tissues in steady state and in organ transplantation, to elucidate mechanisms by which human immune responses are distributed, function and are maintained in the whole body. The proposed studies can uniquely be accomplished by this program through our novel human tissue resource that is the focus of this core. We will use two sources of human samples for the proposed studies in all projects (Projects 1, 2, 3): deceased organ donors and peripheral blood and biopsies from intestinal transplant patients obtained in conjunction with the Columbia Center for Translational Immunology (CCTI) Human studies core. For Service aim 1, we will obtain and process multiple lymphoid and mucosal tissues from human organ donors. We have established the necessary protocols and collaborations with Columbia University Medical Center (CUMC) surgeons and LiveOnNY to obtain from research-consented organ donors, tissues that are not used for life-saving transplantation. We already have the infrastructure set up to obtain multiple primary and secondary lymphoid tissues (bone marrow, thymus, spleen, peripheral, lung- and intestinal draining lymph nodes and tonsils), and mucosal tissues (lungs, small and large intestinal sections) from organ donors whose next-of-kin have consented for the use of tissues for research. The tissues will be processed in the laboratory for obtaining cell suspensions and for preserving tissue sections and cells and/or whole tissues will be distributed to all projects for analysis. For service aim 2, we will coordinate the acquisition of biopsy and peripheral blood samples from transplant patients. In this part of the core, we will obtain biopsy and peripheral blood specimens from intestinal transplant patients who will be enrolled in studies related to Project 3; we will maintain IRB protocols for obtaining samples, enroll patients in this study, and work with the clinicians to obtain biopsy and blood samples, and coordinate storage on unused samples in the CCTI biobank repository. For service aim 3, we will prepare and store tissue sections for imaging by all project laboratories and for a satellite project with Dr. Ron Germain, an intramural investigator at NIAID who will implement his high-dimensional imaging and histocytometry along with advanced computational algorithms to generate an imaging atlas of human immune cells in tissues throughout the body. Together, this core will provide an essential function to this program in the acquisition, processing and distribution of unique human samples to project investigators.

核心B：项目摘要 该计划的主要目标是对人类多样的淋巴细胞种群进行新的分析 稳态和器官移植中的淋巴机和粘膜组织，以阐明机制 人免疫反应分布，功能并保持在整个身体中。提议 该程序可以通过我们新颖的人类组织资源来实现研究，这是 这个核心的重点。我们将在所有项目中使用两个人类样本来源进行拟议的研究（项目 1，2，3）：已故的器官捐献者和肠道移植患者的外围血液和活检 与哥伦比亚转化免疫学中心（CCTI）人类研究核心结合。为了 服务目标1，我们将从人体器官供体中获取并处理多个淋巴和粘膜组织。 我们已经与哥伦比亚大学医学中心建立了必要的协议和合作 （CUMC）外科医生和Liveonny从研究持续的器官捐献者，未使用的组织中获得 用于挽救生命的移植。我们已经设置了基础架构以获得多个主要和 继发性淋巴组织（骨髓，胸腺，脾，周围，肺和肠道排水淋巴 来自器官捐献者 临时的同意使用组织进行研究。组织将在实验室中处理 为了获得细胞悬浮液，并保存组织切片和细胞和/或整个组织将是 分发给所有项目进行分析。对于服务目标2，我们将协调活检和 来自移植患者的外周血样本。在核心的这一部分，我们将获得活检和周围 肠道移植患者的血液标本将参加与项目3有关的研究；我们将 维护IRB方案，以获取样品，参加本研究的患者并与临床医生合作以获得 活检和血液样本，并协调CCTI生物库存储库中未使用的样品的存储。为了 服务AIM 3，我们将准备和存储所有项目实验室和卫星的成像成像的组织部分 NIAID的壁内调查员Ron Germain博士的项目，他将实施其高维度 成像和组织级仪以及先进的计算算法，以生成一个成像地图集 整个身体组织中的人类免疫细胞。在一起，该核心将为 该计划在收购，处理和分发独特的人类样品向项目调查人员中进行了处理。