Human Tissue and Sample Acquisition

人体组织和样本采集

• 批准号: 10426133
• 负责人: Donna L. Farber
• 金额: $ 33.92万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-25 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426133
• 关键词: 3-Dimensional; Academic Medical Centers; Area; Atlases; B-Lymphocytes; Biopsy; Biopsy Specimen; Blood specimen; Bone Marrow; Cells; Clinical Management; Collaborations; Computational algorithm; Consent; Enrollment; Goals; Human; Human Resources; Image; Immune; Immune response; Immune system; Immunology; Infrastructure; Institutional Review Boards; Intestines; Intramural Research; Laboratories; Large Intestine; Life; Lung; Lymphocyte; Lymphoid; Lymphoid Tissue; Methods; Molecular; Mucous Membrane; National Institute of Allergy and Infectious Disease; Organ Donor; Organ Procurements; Organ Transplantation; Patients; Peripheral; Population; Process; Protocols documentation; Research; Research Personnel; Sampling; Savings; Services; Site; Small Intestines; Source; Spleen; Surgeon; Suspensions; System; T cell reconstitution; T memory cell; Thymus Gland; Tissue Preservation; Tissue Sample; Tissue imaging; Tissues; Tonsil; Transplant Recipients; Transplantation; Work; biobank; draining lymph node; gastrointestinal transplantation; high dimensionality; human tissue; metropolitan; novel; peripheral blood; programs; repository; tissue resource

Core B: Project Summary The main goal of this program is to conduct a novel analysis of lymphocyte populations in diverse human lymphoid and mucosal tissues in steady state and in organ transplantation, to elucidate mechanisms by which human immune responses are distributed, function and are maintained in the whole body. The proposed studies can uniquely be accomplished by this program through our novel human tissue resource that is the focus of this core. We will use two sources of human samples for the proposed studies in all projects (Projects 1, 2, 3): deceased organ donors and peripheral blood and biopsies from intestinal transplant patients obtained in conjunction with the Columbia Center for Translational Immunology (CCTI) Human studies core. For Service aim 1, we will obtain and process multiple lymphoid and mucosal tissues from human organ donors. We have established the necessary protocols and collaborations with Columbia University Medical Center (CUMC) surgeons and LiveOnNY to obtain from research-consented organ donors, tissues that are not used for life-saving transplantation. We already have the infrastructure set up to obtain multiple primary and secondary lymphoid tissues (bone marrow, thymus, spleen, peripheral, lung- and intestinal draining lymph nodes and tonsils), and mucosal tissues (lungs, small and large intestinal sections) from organ donors whose next-of-kin have consented for the use of tissues for research. The tissues will be processed in the laboratory for obtaining cell suspensions and for preserving tissue sections and cells and/or whole tissues will be distributed to all projects for analysis. For service aim 2, we will coordinate the acquisition of biopsy and peripheral blood samples from transplant patients. In this part of the core, we will obtain biopsy and peripheral blood specimens from intestinal transplant patients who will be enrolled in studies related to Project 3; we will maintain IRB protocols for obtaining samples, enroll patients in this study, and work with the clinicians to obtain biopsy and blood samples, and coordinate storage on unused samples in the CCTI biobank repository. For service aim 3, we will prepare and store tissue sections for imaging by all project laboratories and for a satellite project with Dr. Ron Germain, an intramural investigator at NIAID who will implement his high-dimensional imaging and histocytometry along with advanced computational algorithms to generate an imaging atlas of human immune cells in tissues throughout the body. Together, this core will provide an essential function to this program in the acquisition, processing and distribution of unique human samples to project investigators.

核心 B：项目摘要 该计划的主要目标是对不同人类的淋巴细胞群进行新的分析 稳态和器官移植中的淋巴和粘膜组织，以阐明其机制 人体免疫反应在全身分布、发挥作用并维持。拟议的 该计划可以通过我们新颖的人体组织资源（即 这个核心的重点。我们将使用两个来源的人类样本来进行所有项目中拟议的研究（项目 1、2、3)：已故器官捐献者以及从肠移植患者获得的外周血和活检组织 与哥伦比亚转化免疫学中心 (CCTI) 人类研究核心合作。为了 服务目标1、我们将从人体器官捐献者身上获取并处理多种淋巴组织和粘膜组织。 我们与哥伦比亚大学医学中心建立了必要的协议和合作 (CUMC) 外科医生和 LiveOnNY 从研究同意的器官捐献者处获取未使用的组织 用于挽救生命的移植。我们已经建立了基础设施来获得多个主要和 次级淋巴组织（骨髓、胸腺、脾脏、外周、肺和肠引流淋巴组织） 淋巴结和扁桃体）以及来自器官捐献者的粘膜组织（肺、小肠和大肠切片） 近亲同意使用组织进行研究。组织将在实验室进行处理 用于获得细胞悬浮液和保存组织切片和细胞和/或整个组织 分发到所有项目进行分析。对于服务目标 2，我们将协调活检和活检的获取 移植患者的外周血样本。在这部分核心中，我们将获得活检和外周血 将参加项目3相关研究的肠道移植患者的血液样本；我们将 维护获取样本的 IRB 协议，将患者纳入本研究，并与临床医生合作以获得 活检和血液样本，并协调 CCTI 生物库存储库中未使用样本的存储。为了 服务目标 3，我们将为所有项目实验室和卫星准备和存储组织切片以供成像 与 NIAID 的校内调查员 Ron Germain 博士合作的项目，他将实施他的高维 成像和组织细胞计数以及先进的计算算法来生成成像图谱 人类全身组织中的免疫细胞。该核心将共同提供基本功能 该计划负责采集、处理和向项目研究人员分发独特的人类样本。