Rapid 3D Whole-Slide Digitization of Thick Cytopathology Slides with a Gigapixel Microscope

使用十亿像素显微镜对厚细胞病理学载玻片进行快速 3D 全载玻片数字化

• 批准号: 10465303
• 负责人: Mark Harfouche
• 金额: $ 100万
• 依托单位: RAMONA OPTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10465303
• 关键词: 3-Dimensional; Academic Medical Centers; Area; Biological Sciences; Biopsy; Bone Marrow Aspiration; Clinical; Collaborations; Computer software; Consumption; Custom; Cytology; Cytopathology; Data; Data Set; Development; Devices; Diagnosis; Diagnostic; Effectiveness; Evaluation; Feedback; Fine needle aspiration biopsy; Fluorescence; Gastrointestinal tract structure; Gold; Hospitals; Image; Image-Guided Surgery; Imagery; Lateral; Light Microscope; Lighting; Location; Lung; Malignant neoplasm of lung; Malignant neoplasm of pancreas; Malignant neoplasm of thyroid; Measurement; Measures; Microscope; Modality; Molecular; Needles; Optics; Pathologist; Pathology; Patient Care; Performance; Persons; Phase; Procedures; Process; Repeat Surgery; Resolution; Robotics; Sales; Sampling; Scanning; Site; Slide; Specimen; Speed; Surgical Pathology; System; Technology; Testing; Thick; Three-Dimensional Image; Thyroid Gland; Time; Universities; Visual; Work; base; biological specimen archives; cancer diagnosis; clinical application; clinically relevant; computerized data processing; design; diagnostic accuracy; digital; digital imaging; digital pathology; experimental study; gigabyte; improved; lens; performance tests; processing speed; sample archive; screening; tool; whole slide imaging

Significance: Digital microscopes are becoming an indispensable tool within the pathology lab. Whole-slide microscope scanners are now routinely used to record gigapixel-sized images of surgical pathology specimens for archival, sharing, annotation and automated processing. Unfortunately, whole-slide scanners still cannot efficiently digitize thick specimens, such as fine-needle aspirates (FNAs) and other cytology samples that are commonly used as the first-line modality for diagnosing cancer of the lung, thyroid, pancreas and other sites. Standard microscope lenses can only capture data from a 1 mm2 area per snapshot. Given this limitation, it is currently not technically feasible to fully scan out an entire slide in 3D, which leads to a number of critical bottlenecks within the cytologist's workflow for cancer diagnosis. Proposal: Ramona Optics is developing a new micro-camera array microscope (MCAM) that can overcome these limitations to digitize thick specimens (up to 50 µm deep) at 1 µm3 volumetric resolution across an entire slide. The resulting multi-gigabyte recording can then be examined by cytopathologists via a custom-developed 3D software interface to aid with various diagnostic tasks. In Phase I of this Fast-Track proposal, Ramona will finalize the hardware and software for its new MCAM-3D device. In Phase II, Ramona will collaborate with the Duke University Medical Center and several other cytopathologists to test and measure MCAM-3D performance on several relevant clinical tasks, including remote telecytology-based assessment of FNA sample adequacy, as well as suitability for secondary diagnosis. Apart from improving workflow and patient care in the hospital, Ramona Optics also expects the MCAM- 3D to enable a number of critical high-throughput imaging experiments in the life sciences that are currently not possible due to the limited throughput of current standard microscope designs. SA1 (Phase I): Integrate hardware and software for whole-slide MCAM-3D capture: Ramona will complete development of an MCAM-3D device that digitizes whole slides (12 cm2 area, 50 µm thick) at 0.8 and 2.6 µm/pixel lateral and axial resolution within 1.5 minutes. 3D viewing software will enable real-time interaction with the multi-gigabyte recorded data volume, offering ~50X more measurements than current 2D whole-slide scanners. SA2 (Phase II): Evaluate MCAM-3D for telecytology and improve system specifications: In collaboration with 3 cytopathologists at the Duke University Medical Center, Ramona will test the MCAM-3D for remote rapid on-site evaluation (ROSE) of sample adequacy. ROSE is well-known to improve patient care by reducing repeat procedures. The objective of this aim is to show that the MCAM-3D can make ROSE easier, quicker and potentially more accurate. At the same time, Ramona will incorporate study findings to improve lateral/axial imaging resolution to 0.5 µm/1 µm and scanning speed to 30 sec. SA3 (Phase II): 3D whole-slide digitization and remote viewing for clinical applications: Ramona will improve the MCAM-3D's processing speeds to enable real-time remote viewing of 3D samples within the Duke University Medical Center. It will then work with cytopathologists to carefully assess its performance at telecytology-based diagnoses and archiving specimen material as it works towards a finalized product for sale 6 months after the conclusion of this project.

意义：数字显微镜是病理学实验室中必不可少的工具 扫描仪通常用于记录用于档案，共享，共享，共享的手术病理标本的千兆像素大小的图像 不幸的是，注释和自动处理。 作为细针抽吸物（FNA）和其他通常用作诊断的一线方式的细胞学样本 肺癌，甲状腺，胰腺和其他标准显微镜镜头。 快照。 wothin的关键瓶颈数量稀薄，细胞学家的工作流叉子用于癌症诊断。 新的微型摄像机阵列显微镜（MCAM）可以克服限制以数字化厚标本（最多50 µmm） 深）在1 µm3的体积分辨率上，横跨玻璃载体。 细胞病理学家通过自定义开发的3D软件接口，以在此快速轨道的第一阶段进行各种诊断任务。 提案，Ramona将在第二阶段的新闻新闻新闻新闻新闻新闻新闻中完成硬件和软件。 与杜克大学医学中心和几个细胞质洛洛斯特群岛一起测试和测量MCAM-3D的性能 几项相关的临床任务，包括基于远程电信的FNA样本是否充分评估 除了改善医院的工作流程和患者护理外，Ramona Optics还希望MCAM- 3D可以在生命科学中启用许多关键的高伸缩成像实验 对于当前标准显微镜设计的有限吞吐量。 SA1（I阶段）：整合硬件和软件，以供全扫描MCAM-3D捕获：Ramona将竞争 在0.8和2.6 µm/像素的横向和 轴向分辨率在1.5分钟内。 体积，提供约50倍的测量值，比当前的2D全滑动扫描仪多。 SA2（II阶段）：评估MCAM-3D电信和改进系统规格：与3合作 Ramona杜克大学医学中心的细胞病理学家将测试MCAM-3D的远程快速现场评估 （玫瑰）的玫瑰是众所周知的，可以通过减少重复程序来改善患者的护理。 目的是购买MCAM-3D可以使玫瑰更轻松，更快，更准确。 将结合研究发现，将LATAL/轴向成像分辨率提高到0.5 µm/1 µm，并扫描速度至30秒。 SA3（II阶段）：3D全面数字化和临床应用的远程查看：Ramona将有所改善 MCAM-3D的处理速度可实时远程查看杜克大学医疗中的3D样品 中心。 该项目结束后6个月，标本材料用于最终产品出售的最终产品。