Core B (Animal Component)

核心B（动物成分）

基本信息

批准号：
10421280
负责人：
JIANMING XU
金额：
$ 23.78万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10421280
关键词：
Adipose tissue Animal Experiments Animal Husbandry Animals Breeding CRISPR/Cas technology Caring Cells Collaborations DNA Data Disease Embryo Embryo Transfer Equipment FOXO1A gene Feeding behaviors Female Fertilization in Vitro Fostering Functional disorder Funding Gene Targeting Generations Genes Genetically Engineered Mouse Genotype Guidelines Homeostasis Human Human Resources Hypothalamic structure Institutes Knock-in Knock-out Liver Maintenance Mediating Mediator of activation protein Messenger RNA Metabolic Metabolic Diseases Metabolic Pathway Metabolism Methodology Methods Molecular Target Mus Mutant Strains Mice Nuclear Receptors Ovulation PPAR alpha PPAR gamma Pathway interactions Physiological Production Program Effectiveness Program Research Project Grants Protein Isoforms Proteins Protocols documentation Regulator Genes Research Research Personnel Resources Role STAT3 gene Savings Services Site System Technology Therapeutic Time Training Transgenes Transgenic Mice Transgenic Organisms United States National Institutes of Health Variant XBP1 gene animal facility animal resource base conditional knockout cost effectiveness embryo cryopreservation embryonic stem cell experience experimental analysis experimental study genotypic sex homologous recombination knock-down male mouse genome mouse model nuclear receptor coactivator 1 operation overexpression pre-clinical preservation programs sex sperm cryopreservation success transcription factor

项目摘要

Core B (Animal Component) - Project Summary All research projects of this Program Project (PP) will rely on the use of genetically engineered mouse (GEM) models to investigate the roles and mechanism of Nuclear Receptors, transcription factors and their coregulators in energy and metabolic homeostasis and disease. More specifically, at least 34 GEM mouse lines will be used, and thousands of male and female mice will be produced by breeding in house during the funding period of this PP. Therefore, this Animal Component of Core B is essential for the success of this PP by integrating expensive equipment, advanced technology, experienced personnel and animal resources. As shown by preliminary studies, Core B has generated/established multiple GEM lines and developed CRISPR-Cas9-mediated mouse gene editing systems. Core B is dedicated to achieving 3 major objectives. Firstly, Core B will use its specialized expertise, state-of-the-art equipment and adequate facilities to generate GEM lines for all investigators of this PP. Core B has established the necessary methods to edit the mouse genome by homologous recombination- based gene targeting in ES cells or CRISPR/Cas9-based gene editing in 1-cell mouse embryos. Core B will generate all GEM lines needed by the investigators of this PP. Secondly, Core B will provide high quality mouse services to manage GEM line colonies and produce genotype-defined and age/sex-matched mice for all investigators of this PP. Specifically, Core B will breed and maintain all of the 34 mouse lines used by this PP. Core B will use our colony expansion protocol to produce large numbers of genotype/age/sex-matched mice for experimental analysis. Core B also will perform genotype analysis, super ovulation, embryo and sperm cryopreservation, and in vitro fertilization services to help the investigators of this PP for managing their mouse colonies, preserving GEM lines, transferring mouse lines between institutes, and saving animal husbandry expenses. Finally, Core B will integrate the efficient usage of animal resources by all investigators of this PP. Because of the large-scale operation of mouse models and the sharing features of multiple mouse lines among the projects of this PP, centralized integration of equipment, expertise and valuable mouse resource will be essential for both scientific success and cost effectiveness. Core B will minimize the expenses of animal experiments by consolidating the usage and management of equipment, expertise, animal facility, mouse maintenance and production. In addition, Core B will serve as a resource for execution and technical training in the use of all animal manipulations needed and oversee the care and use of experimental mice for this PP.

核心 B（动物成分）- 项目摘要该计划项目（PP）的所有研究项目将依赖于基因工程小鼠（GEM）的使用研究核受体、转录因子及其辅助调节剂的作用和机制的模型更具体地说，将使用至少 34 个 GEM 小鼠系，资助期间将通过室内饲养方式培育数千只雄性和雌性小鼠因此，核心 B 的动物成分对于通过整合昂贵的 PP 的成功至关重要。初步显示，设备先进、技术先进、人员丰富、动物资源丰富。研究中，Core B 已生成/建立了多个 GEM 系并开发了 CRISPR-Cas9 介导的小鼠 Core B致力于实现3个主要目标：首先，Core B将利用其专业性。专业知识、最先进的设备和足够的设施，为所有研究人员生成 GEM 线 PP. 核心 B 已经建立了通过同源重组编辑小鼠基因组的必要方法 - 基于 ES 细胞的基因靶向或 1 细胞小鼠胚胎中基于 CRISPR/Cas9 的基因编辑。其次，Core B 将提供高质量的小鼠。管理 GEM 系菌落并为所有小鼠生产基因型定义和年龄/性别匹配的小鼠的服务该 PP 的研究人员。具体来说，Core B 将培育并维护该 PP 使用的所有 34 个小鼠品系。核心 B 将使用我们的菌落扩展方案来生产大量基因型/年龄/性别匹配的小鼠 Core B还将进行基因型分析、超排卵、胚胎和精子分析。冷冻保存和体外受精服务，以帮助该 PP 的研究人员管理他们的小鼠群体、保留 GEM 系、在研究所之间转移小鼠系以及拯救畜牧业最后，Core B 将整合本 PP 所有研究者对动物资源的有效利用。由于鼠标模型的大规模运行以及多条鼠标线之间的共享特性该PP项目，将集中整合设备、专业知识和宝贵资源对于科学成功和成本效益而言至关重要的核心 B 将最大限度地减少动物费用。通过巩固设备、专业知识、动物设施、小鼠的使用和管理进行实验维护和生产。此外，核心 B 将作为执行和技术培训的资源。使用所有所需的动物操作，并监督本 PP 实验小鼠的护理和使用。