CORE--ANIMAL

核心--动物

• 批准号: 7477178
• 负责人: JIANMING XU
• 金额: $ 25.49万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7477178
• 关键词: Adipose tissue; Age; Animal Model; Animals; Applications Grants; Breeding; Cultured Cells; Ensure; Equipment; Family; Fingerprint; Funding; Genes; Genetic; Genotype; Individual; Institutes; Knockout Mice; Liver; MYBBP1A gene; Metabolic; Mus; Numbers; Program Research Project Grants; Research Personnel; Resources; Role; Services; Skeletal Muscle; Steroid Receptors; Training; Transgenic Mice; animal resource; cost; member; mouse model; mutant; nuclear receptor coactivator 1; research study; sex; success

In the last five-year funding period, the investigators of this Program Project Grant (PPG) have focused on the studies for characterizing the genetic and metabolic fingerprints of the three members of the p160 steroid receptor coactivator (SRC) family. In these studies, genetically manipulated mouse models have being extensively used. The Animal Core has met the needs of individual investigators of this PPG by generating a number of new transgenic mouse lines, providing excellent mouse services and sufficient number of genetically manipulated mice for their experiments. In the current proposal, the investigators of this PPG have proposed extensive experiments that are heavily dependent on genetically manipulated mouse models and thereby the role of the Animal Core becomes even more critical than before to the continued success of this PPG. In this proposal, the Animal Core will continue to provide the Investigators of this PPG with the ability to utilize genetically manipulated mouse models and to generate new transgenic mouse lines. In particular, the Animal Core will maintain mouse lines that were produced and used in the last funding period and continuously needed for the next funding cycle, including SRC-1, SRC-2 and SRC-3 knockout mouse lines; the Animal Core will import and re-derive several mouse lines into the Baylor Mouse Facility from other institutes to meet the needs of individual projects of this PPG, including the floxed SRC-1 and the floxed SRC-3 mice; and the Animal Core will generate new transgenic mouse lines to satisfy the needs of individual investigators for their studies with spatial and temporal inactivation of specific genes in the adipose tissue, liver, skeletal muscle and cultured cells derived from our mutant/transgenic mice. Furthermore, the Animal Core will breed, produce and provide age, sex and genotype-matched mice to individual investigators for their experiments. In addition, the Animal Core will serve as a resource for execution and training in the use of all animal manipulations needed in this PPG proposal. The Animal Core will minimize the cost of utilizing animal models in this PPG by consolidating the equipment, expertise and animal resources, ensuring an efficient use of animal resources at minimal cost.

在最后五年的资金期间，该计划项目赠款（PPG）的调查人员重点介绍 表征P160类固醇三个成员的遗传和代谢指纹的研究 受体共激活因子（SRC）家族。在这些研究中，遗传操纵的小鼠模型是 广泛使用。动物核心通过产生了该PPG的个别调查人员的需求 许多新的转基因鼠标线，提供出色的鼠标服务和足够的数量 遗传操纵小鼠进行实验。在当前的建议中，该PPG的调查人员 已经提出了广泛的实验，这些实验在很大程度上取决于遗传操纵的小鼠模型 因此，动物核心的作用变得比以前更为关键 这个ppg。在该提议中，动物核心将继续向该PPG的调查人员提供 能够利用遗传操纵的小鼠模型并生成新的转基因小鼠系。在 特别是，动物核心将维护在最后一段资金期间生产和使用的鼠标线 在下一个融资周期中需要不断需要，包括SRC-1，SRC-2和SRC-3敲除鼠标 线；动物核心将从其他 学院以满足PPG的各个项目的需求，包括Floxed Src-1和Floxed SRC-3小鼠；动物核心将产生新的转基因小鼠线，以满足 个人研究者的研究，以脂肪中特定基因的空间和时间灭活 源自我们的突变/转基因小鼠的组织，肝脏，骨骼肌和培养细胞。此外， 动物核心将繁殖，生产并提供年龄，性别和基因型匹配的小鼠 为了实验。此外，动物核心将作为执行和培训的资源 在此PPG提案中使用所有动物操纵。动物核心将使成本降至最低 通过合并设备，专业知识和动物资源，利用该PPG中的动物模型， 确保有效利用动物资源，费用最低。