ACQUISITION OF HIGH-THROUGHPUT 200 kV CRYO-TEM

获取高通量 200 kV 冷冻透射电镜

• 批准号: 10415650
• 负责人: Irina I Serysheva
• 金额: $ 200万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415650
• 关键词: Area; Bacterial Drug Resistance; Cell membrane; Collaborations; Communicable Diseases; Computer software; Contract Services; Core Facility; Cost Sharing; Cryoelectron Microscopy; Electrons; Equipment; Fostering; Funding; Generations; Health Sciences; Image; Institution; Malignant Neoplasms; Medical center; Microscope; Neurosciences; Productivity; Research; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Specimen; Students; Texas; Time; Titan; Transmembrane Transport; United States National Institutes of Health; Universities; cost; design; detector; drug discovery; high resolution imaging; imaging facilities; instrument; medical schools; screening; structural biology; transmission process

Project Summary/Abstract The proposal is to request support for the purchase of the 200 kV Glacios Transmission Electron Cryo- microscope from Thermo Fisher Scientific Inc.. The requested instrument will replace our current Tecnai G2 Polara (300 kV) cryomicroscope, which was purchased nearly two decades ago and is currently at the end of its usefulness. At this time, Thermo Fisher does not provide service contract for the Polara and to keep the microscope operational is becoming a challenge that jeopardizes our investigators and their NIH funded projects. Strong institutional support is provided with a cost-sharing commitment of $1,000,000 to the purchase of the proposed Glacios and another ~$1,000,000 for over five years to support the projected operational costs of the new cryo-TEM. The requested instrument will be integrated within the Cryo-EM Core Facility established under the umbrella of the Structural Biology Imaging Center at McGovern Medical School at the University of Texas Health Science Center (UTHealth) at Houston. UTHealth’s Cryo-EM Core Facility is a well administered, highly collaborative and user focused cryo-EM resource currently equipped with a Tecnai G2 Polara and a Titan Krios G3. A Glacios is a new generation microscope offered by Thermo Fisher that will replace our obsolete Polara while at the same time offering significant technological improvements and enabling new capabilities for our users. That includes high-throughput specimen optimization and screening through the integrated EPU software, which can be followed by contamination-free sample transfer to the Titan Krios for high-resolution imaging; optimized cryo-EM imaging using the latest generation of electron counting detector Falcon 4 integrated with the new Selectris energy filter, designed for stability and ease-of-use. The proposed Glacios will serve the needs of over two dozen research projects in key areas such as cancer, neuroscience, infectious diseases, drug discovery, antibacterial drug resistance, cell membrane transport, and others. It will deeply impact the research in the Texas Medical Center and surrounding institutions by: increasing scientific productivity; fostering interactions and collaborations amongst scientists, strengthening the competitiveness for research funding, and boosting our ability to attract new investigators and students by the prospect of using start-of-the-art cryo-EM equipment.

项目摘要/摘要 该提案是要求购买200 kV冰川传输电子冷冻 - Thermo Fisher Scientific Inc.的显微镜。所请求的仪器将取代我们当前的Tecnai G2 Polara（300 kV）的冷冻球体镜，该冷冻镜是在大约二十年前购买的，目前正处于 它的有用性。目前，Thermo Fisher不提供Polara的服务合同，并保留 显微镜操作正成为危害我们的调查人员及其NIH资助的挑战 项目。为购买的成本分布的承诺提供了强大的机构支持 在拟议的冰川和五年内另外约$ 1,000,000左右，以支持预计的运营成本 新的低温战。请求的仪器将集成在建立的冷冻EM核心设施中 在大学麦戈文医学院的结构生物学成像中心的伞下 休斯敦的德克萨斯健康科学中心（UTHEADY）。 Uthealth的Cryo-Em核心设施是一个很好的管理， 当前配备了Tecnai G2 Polara和A Titan Krios G3。 Glacios是Thermo Fisher提供的新一代显微镜，它将取代我们 过时的polara同时提供了重大的技术改进并启用了新的 我们用户的功能。其中包括高通量标本的优化和通过 集成的EPU软件，随后可以将无污染的样品转移到Titan Krios 高分辨率成像；使用最新一代电子计数检测器优化的冷冻EM成像 Falcon 4与新的Selectris Energy滤光片集成，旨在稳定性和易用性。提议 Glacios将满足癌症，神经科学，神经科学等关键领域的二十多个研究项目的需求 传染病，药物发现，抗菌耐药性，细胞膜运输等。会 对德克萨斯医疗中心及周边机构的研究深深影响：增加科学 生产率;促进科学家之间的互动和合作，增强了竞争力 研究资金，并提高我们吸引新调查人员和学生的能力 开始冷冻EM设备。