Precision Histology

精密组织学

• 批准号: 10413912
• 负责人: PARTHA Pratim MITRA
• 金额: $ 45.63万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413912
• 关键词: 3-Dimensional; Anatomy; Atlases; Brain; Brain Stem; Data; Data Set; Exploratory Behavior; Histologic; Histology; Image; Individual; Label; Laboratories; Lead; Methods; Motor; Mus; Neurons; Population; Research Project Grants; Reverse engineering; Slice; Spinal; Stains; Standardization; System; Texture; Tissues; Training; Transgenic Mice; base; brain tissue; cell type; digital; experimental study; histological stains; in vivo; motor control; novel marker; olfactory bulb; operation; orofacial; reconstruction

PIs: Martin Deschênes, Yoav Freund, David Golomb, David Kleinfeld (lead), Fan Wang ! Core 2. Abstract Precision Histology This Core provides high-throughput tape transfer sectioning to cut near perfect stacks of slices of the whole brain, from olfactory bulb to spinal level C2. The tape transfer histology system has been in continuous operation in the Mitra laboratory for several years and will enable the registration of anatomical data from Research Projects 1, 2, and 3, into a common framework established by the Trainable Texture-based Digital Atlas. Control of orofacial actions is coordinated by distinct populations of brain stem premotor neurons that are arranged into relatively small clusters and limited to domains as small as 200 to 300 µm in extent. For many orofacial motor actions, premotor neuronal clusters are present at multiple levels of the brainstem and do not conform to the boundaries previously defined by available atlases, including the Paxinos atlases and the Allen Brain Common Coordinate Framework atlas. As part of our concerted effort to identify the functional organization of brainstem motor control, we require standardized processing of brain tissue. The established histology pipeline in the Mitra laboratory offers key advantages over alternative methods, in that tissue is retained in near perfect alignment during sectioning and is amenable to standard histological stains that demarcate brainstem landmarks used to align and annotate brains. Crucially, this approach precludes distortions from classical sectioning methods and enables efficient 3D reconstructions, cross-brain registration, and efficient incorporation of data from new brains into our recently developed Trainable Texture-based Digital Atlas. By efficiently multiplexing labeled neurons from transsynaptic tracing, in vivo recordings, and transgenic mice/cell type marker stains, the Precision Histology Core serves to support data compilation into a common anatomical framework.

PIS：MartinDeschê​​nes，Yoav Freund，David Golomb，David Kleinfeld（铅），Fan Wang 呢 核心2。摘要 精密组织学 该核心提供高通量胶带传输部分，以切割整个整个切片的完美堆栈 大脑，从嗅球到脊柱C2。磁带转移组织学系统一直处于连续状态 在MITRA实验室运行几年，将从 研究项目1、2和3，是由基于训练的数字纹理建立的共同框架 地图集。 对口面作用的控制由不同的脑干前神经元的不同种群进行协调 排列成相对较小的簇，限制为小至200至300 µm的域。对于许多人 口腔运动动作，前神经元簇在脑干的多个层次上存在，不 符合先前由可用地图的界限，包括Paxinos地图集和艾伦 大脑共同坐标框架图集。 作为确定脑干电机控制功能组织的一致努力的一部分，我们需要 脑组织的标准化加工。 MITRA实验室中既定的组织学管道提供了关键 优于替代方法的优势，在该组织中，该组织在切片期间几乎保持完美的对准和 适用于标准的组织学染色，这些迹象将用于对齐和注释的脑干地标划定脑干地标 大脑。至关重要的是，这种方法排除了经典截面方法的扭曲，并使有效 3D重建，跨脑注册和有效地纳入新大脑的数据 最近开发了可训练的基于纹理的数字地图集。通过有效地将标记的神经元复量 跨突触追踪，体内记录和转基因小鼠/细胞类型标记染色，精确的组织学 核心将数据汇编支持到一个常见的解剖框架中。