Core D: Digital Pathology and CyTOF

核心 D：数字病理学和 CyTOF

• 批准号: 10408089
• 负责人: ROBERT A. ANDERS
• 金额: $ 34.28万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10408089
• 关键词: Animals; Bar Codes; Bioinformatics; Biological; Biological Assay; Biopsy Specimen; Cells; Clinical; Color; Communities; Complex; Comprehensive Cancer Center; Cytometry; Data; Data Set; Development; Ensure; Equipment; Fibroblasts; Flow Cytometry; Formalin; Generations; Genetic Transcription; Goals; Grant; Histopathology; Housing; Human; Human Resources; Image Analysis; Imaging Techniques; Immune; Immune System Diseases; Immune response; Immunofluorescence Immunologic; Immunohistochemistry; Immunotherapeutic agent; Infrastructure; Institutes; Internet; Laboratories; Link; Location; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Malignant neoplasm of pancreas; Maps; Methodology; Methods; Microscope; Molecular Profiling; Mus; Myelogenous; Myeloid Cells; Myeloid-derived suppressor cells; Oncology; Operative Surgical Procedures; Outcome; Pancreatic Ductal Adenocarcinoma; Paraffin Embedding; Pathologist; Pathology; Physicians; Productivity; Program Research Project Grants; Proteins; Proteomics; Quality Control; Research; Research Personnel; Research Project Grants; Resolution; Resources; Rest; Sampling; Scanning; Scientist; Stains; Suppressor-Effector T-Lymphocytes; Suspensions; System; T cell receptor repertoire sequencing; T-Lymphocyte; Technology; Time; Tissue Sample; Tissue Stains; Tissues; Training; Tumor Tissue; Validation; Western Blotting; Work; anticancer research; biomarker panel; cancer immunotherapy; data acquisition; design; digital pathology; high dimensionality; liquid crystal polymer; mid-career faculty; multimodality; multiplexed imaging; pancreatic neoplasm; professor; programs; protein biomarkers; protein profiling; quality assurance; response; single cell proteins; single-cell RNA sequencing; stem; success; targeted treatment; tool; translational study; tumor; tumor immunology; tumor microenvironment; tumor-immune system interactions

PROJECT SUMMARY The Digital Pathology and Mass Cytometry Core (Core D) will provide state-of-the-art technologies to enable high-parameter single-cell proteomic profiling for all the Research Projects in the proposed P01 Program Project Grant. The core directors are experts in multicolor immune profiling (multispectral immunoflurescence staining) and high-dimensional proteomic profiling (Cytometry by Time-of-Flight; CyTOF) technologies and will work with project investigators to assay human and mouse tissues generated over the five years of the Program Project. Dr. Anders (core co-director) is an Associate Professor of Pathology, is a practicing GI surgical pathologist who co-directs the Tumor Microenvironment (TME) Laboratory in the Bloomberg Kimmel Institute for Cancer Immunotherapy. The core houses approximately 6 million dollars of equipment aimed at multicolor tissue staining and analysis. The major equipment includes 2 Leica Bond automated immunostainers, 6 Akoya automated high capacity multichannel high-resolution scanning microscopes (Vectra and Polaris systems), and 8 data servers linked by 10G and 40G internet connections to the rest of the campus. The TME laboratory is a central resource for multispectral immunofluorescence staining and image analysis for the Johns Hopkins Oncology research community. Dr. Ho (core co-director) is an Assistant Professor of Oncology and Scientific Director of the newly established CyTOF core, housing the third-generation cytometer Helios™, in the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins. Dr. Ho completed his postdoctoral research in the laboratory of Dr. Elizabeth Jaffee with a special emphasis on developing mass cytometry pipelines to profile the immunologic responses. Dr. Ho also co-led the development of analytical pipelines for CyTOF datasets with Dr. Elana Fertig (Bioinformatics and leader for Core C). Both directors, Anders and Ho, have demonstrated a strong track record of productivity utilizing these technologies. The lab spaces and offices dedicated to this Core are located in close proximity within the Cancer Research Building Complex. The goal of the Digital Pathology and Mass Cytometry Core (Core D) is to provide established and validated assay platforms. The Core Directors have already set-up the assay systems and validated specific marker panels. This provides quality assurance and control for assays performed within and across projects. Once acquired, the data will be provided to Core C for analysis and integration, and to Core B for clinical correlations.

项目摘要 数字病理和质量细胞仪核心（核心D）将提供最先进的技术以实现 拟议的P01计划项目中所有研究项目的高参数单细胞蛋白质组学分析 授予。核心主管是多色免疫荧光染色的专家 和高维蛋白质组学分析（按照飞行时间； Cytof）技术，将与 在计划项目的五年中，项目研究人员测定人类和小鼠组织。 Anders博士（核心联合导演）是病理学副教授，是一位实用的GI手术病理学家，他 在彭博金梅尔癌症研究所共同导向肿瘤微环境（TME）实验室 免疫疗法。核心房屋大约有600万美元的设备，旨在多色组织染色 和分析。主要设备包括2个Leica Bond自动免疫击败器，6个Akoya自动化高 容量多通道高分辨率扫描显微镜（Vectra和Polaris系统）和8个数据服务器 由10G和40G互联网连接到校园其他地区。 TME实验室是中心资源 对于约翰·霍普金斯肿瘤学研究的多光谱免疫荧光染色和图像分析 社区。 Ho（核心联合主任）博士是肿瘤学助理教授和新近的科学主任 建立的Cytof Core，在Sidney Kimmel综合中容纳第三代细胞仪Helios™ 约翰·霍普金斯（Johns Hopkins）的癌症中心。 Ho博士在伊丽莎白博士的实验室完成了博士后研究 Jaffee特别强调开发质量细胞仪管道以介绍免疫学反应。 Ho博士还与Elana Fertig博士共同领导了Cytof数据集的分析管道的开发 （生物信息学和核心C的领导者）。董事，安德斯（Anders）和霍（Ho）都表现出良好的往绩 利用这些技术的生产力。专门针对此核心的实验室空间和办公室位于近距离 癌症研究大楼中的接近性。 数字病理学和质量细胞仪核心（核心D）的目标是提供已建立和验证的 测定平台。核心主管已经设置了分析系统并验证了特定标记 面板。这为在项目内部和跨项目中执行的测定提供了质量保证和控制。一次 获取后，数据将提供给核心C进行分析和集成，并向核心B提供临床B的核心B 相关性。