KULMAP: Human Kidney, urinary tract and lung mapping center

KULMAP：人类肾脏、泌尿道和肺部绘图中心

• 批准号: 10413584
• 负责人: Sanjay Jain
• 金额: $ 10万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-25 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10413584
• 关键词: 3-Dimensional; Ablation; Atlases; Blood Pressure; Blood Vessels; Chronic Kidney Failure; Clinical Trials; Data; Diabetes Mellitus; Disease; Epithelial Cells; Foundations; Goals; Homeostasis; Human; Human BioMolecular Atlas Program; Hypertension; Image; Immunofluorescence Microscopy; Kidney; Knowledge; Lung; Lymphatic; Lymphovascular; Maps; Metabolic; Microscopy; Nerve; Neuroeffector Junction; Obstruction; Organ; Pathologic; Resources; Role; Sensory; Site; Structure; System; Tissues; Urinary tract; Visualization; cell type; glomerular filtration; immunoregulation; innovation; interest; neurovascular; prevent; scaffold; technological innovation

Project Summary: The kidney nerves and lymphatics use blood vessels as conduits to reach their target sites of action including the vasculature, epithelial cells and interstitium. The lymphatics have important roles in immunomodulation and the kidney sensory and sympathetic nerves closely interact with target sites to regulate glomerular filtration, blood pressure and sense imbalances in metabolic derangements or physical obstruction. A three-dimensional (3D) atlas of regional and cellular distribution and neuroeffector sites in healthy kidneys is essential to understand the different pathological conditions and diseases due to their abnormal activities. However, these structures are not currently being studied in the kidney and pose a specific challenge due to the need for their assessment in meso (cm) and micro (microns) scale. The purpose of this supplement is to generate 3D-volumetric imaging data of sympathetic and sensory renal nerves and their relationship with the blood vessels, lymphatics and functionally important target cell types by leveraging resources of SPARC, HuBMAP and our technological innovations in applying 3D multiplexed immunofluorescence microscopy on large pieces of kidney tissue using Lightsheet microscopy. The neurovascular segmentation, scaffold building and quantitative visualization provided by SPARC will pave the way for broader efforts of similar goals for other HuBMAP organs. The importance of a spatial neurovascular atlas is of prime interest as targeted renal nerve ablation approaches are in clinical trials to treat hypertension and prevent progression of chronic kidney diseases.

项目摘要：肾神经和淋巴管使用血管作为导管 目标位点，包括脉管系统，上皮细胞和间质。淋巴管有 在免疫调节以及肾脏感觉和交感神经紧密相互作用中的重要作用 与目标部位调节肾小球滤过，血压和代谢中的感觉失衡 危险或身体障碍。区域和细胞的三维（3D）地图 健康肾脏中的分布和神经索夫妇位点对于了解不同的不同 病理状况和疾病由于其异常活动而引起的疾病。但是，这些结构是 目前尚未在肾脏中研究并构成特定的挑战，因为他们需要 在中期（CM）和微（微米）量表中评估。该补充的目的是生成 交感神经和感觉肾神经的3D量化成像数据及其与 血管，淋巴管和功能上重要的靶细胞类型，利用 SPARC，HUBMAP和我们应用3D多重免疫荧光的技术创新 使用灯表显微镜在大型肾脏组织上的显微镜检查。神经血管 SPARC提供的分割，脚手架构建和定量可视化将为 其他Hubmap器官类似目标的更广泛的努力。空间神经血管的重要性 地图集是主要的兴趣，因为有针对性的肾神经消融方法正在临床试验中 高血压并预防慢性肾脏疾病的进展。