Laboratory Sciences Core (Core D)

实验室科学核心（核心 D）

• 批准号: 10404938
• 负责人: Spyros A Kalams
• 金额: $ 33.29万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10404938
• 关键词: Academic Medical Centers; Acquired Immunodeficiency Syndrome; Address; Adipose tissue; Administrative Supplement; Adoption; Area; B-Cell Antigen Receptor; B-Lymphocytes; Basic Science; Biological Assay; Biomass; Cell Separation; Cell physiology; Cells; Cellular Indexing of Transcriptomes and Epitopes by Sequencing; Clinical; Clinical Sciences; Collaborations; Consultations; Core Facility; Cryopreservation; DNA; DNA sequencing; Discipline; Disease; Equipment; Flow Cytometry; Funding; Future; Gene Expression Profiling; Genomics; Grant; HIV; HIV Genome; Health; Immunology; In Vitro; Individual; Laboratories; Leadership; Lymphocyte; Measures; Metagenomics; Methodology; Mission; Nucleic Acids; Pathogenesis; Performance; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Population; Procedures; Proteins; Protocols documentation; RNA; Receptor Cell; Recording of previous events; Research; Research Design; Research Personnel; Resources; Ribosomal RNA; Role; Saliva; Sampling; Schedule; Science; Sequence Analysis; Services; Skin; Sorting - Cell Movement; Specimen; Stains; System; T cell receptor repertoire sequencing; T-Lymphocyte; Techniques; Technology; Tennessee; Tissues; Training; Translational Research; Viral Genome; Work; career development; design; digital; genetic variant; genomic platform; host microbiome; human genomics; innovation; integration site; laboratory experience; medical schools; microbial; microbial genomics; microbiome analysis; microbiome sequencing; nano-string; single cell sequencing; success; synergism; transcriptomics; viral genomics

Laboratory Sciences Core – PROJECT SUMMARY The CFAR Laboratory Sciences (LSC) provides services specifically designed to meet the needs of HIV clinical, translational and basic science researchers. Over the past grant cycle, we established multiple services for CFAR investigators, with an easily accessible scheduling and chargeback system, regardless of investigators' prior level of laboratory experience or training. These include cell isolation and cryopreservation protocols for PBMCs, adipose tissue, and skin. We developed cell staining protocols for analytical flow cytometry to evaluate the phenotype and function of B and T lymphocytes, and flow sorting assays to bulk sort populations of lymphocytes, or to perform single-cell sorting for detailed transcriptomic analyses. Vanderbilt has long prioritized institutional support for shared core facilities and invested accordingly. The services that the LSC established over the prior funding cycle were carefully designed to address needs unmet by other non-CFAR Vanderbilt core facilities, while simultaneously taking advantage of the impressive range of institutional cores that are equally available to investigators at Vanderbilt and Meharry. Our successes have allowed us to anticipate the future needs of this CFAR. An institutional core, VANTAGE (VANderbilt Technologies for Advanced Genomics), has significantly enhanced our ability to analyze single cells, including B cell receptor and T cell receptor sequencing, and RNA transcriptional profiling. VANTAGE has also designed assays to perform whole HIV genome, including integration site, sequencing. The LSC, in addition to providing its own services, will function as a concierge service. We will help investigators design appropriate flow cytometry panels, and sort cells, and leverage VANTAGE to perform sequencing and analysis. The mission of the LSC is to continue to provide sustainable leadership and services specifically responsive to the needs of HIV investigators, so that these services are used in ways unimaginable without CFAR. The LSC will work closely with CFAR investigators to design assays, facilitate assay performance, and help interpret results. The LSC will promote basic, clinical and translational HIV research through two Specific Aims: 1) To provide expertise, consultation, assay performance, and training in immunology techniques including those that require BSL3 procedures. These include study design and implementation of state-of-the-art assays to measure phenotype and function of cell subsets relevant to HIV and to quantitate HIV protein and nucleic acid in plasma samples and in vitro culture. 2) To provide concierge level service for genomics research relevant to HIV. Services include access to single-cell transcriptomics technologies, viral genome evolutionary sequence analysis, and host microbiome analysis relevant to HIV disease pathogenesis.

实验室科学核心 - 项目摘要 CFAR实验室科学（LSC）提供专门设计的服务，以满足HIV临床的需求， 翻译和基础科学研究人员。在过去的赠款周期中，我们为 CFAR调查人员，具有易于访问的调度和退款系统，无论研究人员如何 先前的实验室经验或培训水平。这些包括细胞隔离和冷冻保存方案 PBMC，脂肪组织和皮肤。我们开发了用于分析流式细胞仪的细胞染色方案以评估 B和T淋巴细胞的表型和功能，以及流动分类测定法 淋巴细胞，或进行单细胞分类以进行详细的转录组分析。范德比尔特长期以来优先考虑 机构对共享核心设施的支持并进行了相应的投资。 LSC建立的服务 在先前的融资周期中，精心设计了其他非粉丝范德比尔特核心未满足的需求 设施，同时利用同样具有令人印象深刻的机构核心 范德比尔特和梅哈里的调查人员可供选择。我们的成功使我们能够预期未来 此CFAR的需求。机构核心，Vantage（Vanderbilt技术的高级基因组学技术），已有 显着增强了我们分析单个细胞的能力，包括B细胞受体和T细胞受体测序， 和RNA转录分析。 Vantage还设计了执行整个HIV基因组的测定法，包括 集成站点，测序。除了提供自己的服务外，LSC还将充当礼宾 服务。我们将帮助研究人员设计适当的流式细胞仪面板，并分类细胞，并利用 进行测序和分析的有利位置。 LSC的使命是继续提供可持续性 领导和服务特别响应艾滋病毒调查人员的需求，以便使用这些服务 在没有CFAR的情况下无法想象的方式。 LSC将与CFAR调查人员紧密合作，以设计测定法， 促进测定性能，并有助于解释结果。 LSC将促进基本，临床和翻译 艾滋病毒通过两个具体目标进行研究：1）提供专业知识，咨询，评估绩效和培训 在包括需要BSL3程序的免疫学技术中。这些包括研究设计和 实施最先进的测定法，以测量与艾滋病毒和艾滋病毒相关的细胞子集的表型和功能 定量血浆样品和体外培养中的HIV蛋白和核酸。 2）提供礼宾水平 与艾滋病毒有关的基因组学研究服务。服务包括访问单细胞转录组学 技术，病毒基因组进化序列分析和与HIV相关的宿主微生物组分析 疾病发病机理。