Microbiology and Metagenomics Core

微生物学和宏基因组学核心

• 批准号: 10404439
• 负责人: Richard L Gallo
• 金额: $ 39.22万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10404439
• 关键词: 16S ribosomal RNA sequencing; Acne; Adult; Anaerobic Bacteria; Antibiotic Resistance; Antimicrobial Resistance; Atopic Dermatitis; Awareness; Bacteria; Behavior; Bioinformatics; Biological; Cells; Childhood; Clinical; Clinical Research; Collaborations; Complex; Coupled; DNA; Data; Data Analyses; Data Set; Disease; Education; Elements; Ensure; Equipment and supply inventories; Event; Fermentation; Fibroblasts; Functional disorder; Generations; Genes; Genome; Genomics; Goals; Growth; Human; Immune response; Libraries; Metabolic; Metagenomics; Methodology; Microbe; Microbial Biofilms; Microbial Genetics; Microbiology; Modeling; Mus; Netherton syndrome; Organism; Patients; Phenotype; Procedures; Process; Production; Protocols documentation; Psoriasis; Publishing; Resistance profile; Resolution; Resources; Ribosomal RNA; Rosacea; Sampling; Shotguns; Site; Skin; Software Tools; Standardization; Swab; Taxonomy; Techniques; Technology; Testing; Toxin; Training; Training and Infrastructure; Translational Research; Validation; Work; cost effective; experience; gene function; genetic information; in vivo; insight; keratinocyte; large datasets; lipid metabolism; lipidome; lipidomics; meetings; member; metabolome; metabolomics; metagenome; microbial; microbial community; microbiome; microbiome research; multiple omics; next generation; next generation sequencing; pathogen; quorum sensing; screening; sequencing platform; skin microbiome; skin microbiota; synergism; transcriptome; transcriptomics

PROJECT SUMMARY/ABSTRACT Microbiology and metagenomics core The purpose of this core is to facilitate high-throughput characterization of the acne microbiome from clinical samples of acne patients generated from the Acne Center of Research Translation (CORT), and to advance analysis of the function of these microbes beyond basic genomic characterization. Next-generation sequencers have transformed microbiome research but have proven insufficient to understand the biologic relevance of this microbial genetic information in the skin. The acne microbiome has been partially characterized but without understanding of how products of these diverse organisms influence the pathophysiology of acne. The Core Director, Core Associate Director, and staff of this core have extensive experience in the generation and analysis of metagenomic data and have successfully developed a panel of functional screens to enable analysis of how target cells respond to the metabolic products of microbe. This requires expertise in the study of C. acnes, the predominant bacterial species in acne that is anaerobic and fastidious, thus requiring expertise for isolation and culture. C. acnes produces a range of fermentation metabolites and other products that can alter host immune responses and the growth of other organisms. This core will produce libraries of bacterial organisms from the skin, perform metagenomic analysis from skin swabs, and functionally screen libraries for their capacity to alter the immune response of fibroblasts, keratinocytes and immunocytes relevant to the skin of acne subjects, and analyze these observations to generate hypothesis for further testing. The core will also work closely with the transcriptomics core and lipidomic screens at UCLA to further integrate data for validation and discovery. This ability will transform the utility of the data to members of the CORT.

项目概要/摘要 微生物学和宏基因组学核心 该核心的目的是促进临床痤疮微生物组的高通量表征 痤疮研究翻译中心 (CORT) 生成痤疮患者样本，并推进 对这些微生物功能的分析超出了基本的基因组特征。下一代测序仪 改变了微生物组研究，但事实证明不足以理解微生物组的生物学相关性 这种微生物的遗传信息存在于皮肤中。痤疮微生物组已部分表征，但 不了解这些不同生物体的产物如何影响痤疮的病理生理学。这 核心总监、核心副总监以及该核心的工作人员在生成和构建方面拥有丰富的经验。 分析宏基因组数据，并成功开发了一组功能屏幕，以实现 分析靶细胞如何响应微生物的代谢产物。这需要研究方面的专业知识 痤疮丙酸杆菌是痤疮中的主要细菌种类，厌氧且挑剔，因此需要专业知识 用于隔离和文化。痤疮丙酸杆菌产生一系列发酵代谢物和其他产物， 改变宿主的免疫反应和其他生物体的生长。该核心将产生细菌文库 从皮肤中提取生物体，从皮肤拭子中进行宏基因组分析，并对文库进行功能筛选 它们改变与皮肤相关的成纤维细胞、角质形成细胞和免疫细胞的免疫反应的能力 痤疮受试者，并分析这些观察结果以产生进一步测试的假设。核心还将 与加州大学洛杉矶分校的转录组学核心和脂质组学筛选密切合作，进一步整合数据进行验证 和发现。这种能力将把数据的效用转变为 CORT 成员。