Acquisition of HyPix-6000HE Hybrid Photon Counting Detector and Universal Kappa 4-Axis Goniometer for the X-Ray diffraction system

采购用于 X 射线衍射系统的 HyPix-6000HE 混合光子计数探测器和通用 Kappa 4 轴测角仪

• 批准号: 10399707
• 负责人: Lawrence E Cornett
• 金额: $ 22.69万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10399707
• 关键词: 17 year old; 2019-nCoV; Academic Medical Centers; Academy; Algorithms; Anodes; Architecture; Arkansas; Award; Beryllium; Biological; Biomedical Research; Biotechnology; Budgets; California; Catalogs; Centers of Research Excellence; Characteristics; Charge; Chemicals; Chemistry; Chicago; Communication; Computer software; Computers; Core Facility; Country; Coupled; Critical Thinking; Cryoelectron Microscopy; Crystallization; Crystallography; Curiosities; Data; Data Collection; Data Set; Deposition; Devices; Disasters; Disease; Doctor of Philosophy; Drug Targeting; Educational process of instructing; Ensure; Enzymatic Biochemistry; Equipment; Faculty; Family; Fees; Funding; Future; Generations; Government; Grant; Health; Heart; Human; Hybrids; In Situ; Individual; Industry; Institution; Investments; Laboratories; Lead; Learning; Left; Legal patent; Letters; Licensing; Life; Light; Maintenance; Manufacturer Name; Measurement; Measures; Molybdenum; National Center for Research Resources; Noise; Oxygen; Peptide Hydrolases; Photons; Play; Postdoctoral Fellow; Powder dose form; Price; Privatization; Process; Proteins; Reaction; Research; Research Personnel; Resolution; Roentgen Rays; Role; Running; Sampling; Saturn; Schools; Science; Services; Signal Transduction; Site; Source; Structural Models; Structure; Students; Synchrotrons; System; Taste Perception; Technology; Temperature; Time; Training Support; Translational Research; Twin Multiple Birth; United States; United States National Institutes of Health; Universities; Update; Vendor; Writing; X ray diffraction analysis; X-Ray Crystallography; base; beamline; career; catalyst; cost; density; design; detector; experimental study; improved; inhibitor/antagonist; instrument; instrumentation; macromolecule; member; metalloenzyme; new technology; novel; photon-counting detector; pressure; prevent; programs; protein data bank; screening; skills; small molecule; student training; technology validation; three dimensional structure; tool; undergraduate research; undergraduate research experience; undergraduate student; usability; voucher; web site

PROJECT SUMMARY/ ABSTRACT The X-ray diffraction system, in the laboratory of Prof. Joshua Sakon (PI X-ray crystallography core), has played and will continue to play a key role in the statewide research efforts in the structure determination of biomolecules. His involvement in the Arkansas INBRE began in 2001 when the Arkansas BRIN was first funded through a grant from the National Center for Research Resources. Dr. Sakon co-directs the Biotechnology Core and in that role helps Arkansas INBRE network faculty and students access University of Arkansas core facilities. This budget is for the acquisition of the HyPix-6000HE Hybrid Photon Counting Detector and Universal Kappa 4-Axis Goniometer for the X-Ray diffraction system. The instrumentation will enhance user access to new technology while replacing outdated equipment that is no longer serviceable by the manufacturer. With the new installation, the deinstallation of Saturn-92 and AFC9, Beryllium window of left port will also be required. The system, which was purchased in 2003, has provided an excellent return on investment for 17 years. Eighty-one structures solved in the facility are validated and made accessible from the Protein Data Bank (PDB). The facility provided 78.4% of three-dimensional structures deposited from our state of Arkansas. During the period 2003−2021, the coordinates were frequently downloaded (1,685,267 times) and examined using the website tools implemented by PDB (503,322 times) and led our state in impact (87.9% of downloads and views). These structures are aiding in understanding protein architectures and designing new molecules. The detector/goniometer portion of the system is in desperate need of upgrade. However, the old detector is no longer supported by the manufacturer Replacing the existing detector/goniometer portion of the system will extend the life of the X-ray diffraction system as well as increase the functionality and usability of the system. The new generation X-ray detector will produce higher quality data and the software that now comes with the detector/goniometer replacement will leverage advanced new algorithms developed in recent years and will be capable of extracting more accurate information even with the same signal to noise ratio. The investment will extend the capacity of the instrument from being a system dedicated to protein crystallography to one that is capable of also generating high-quality analytics for small molecules. With the proposed upgrade of the system, for less money than a new small molecule crystallography system, the facility can continue to serve the needs of INBRE-, COBRE-, and as well as other NIH-supported investigators on this campus and across the state.

项目概要/摘要 X 射线衍射系统位于 Joshua Sakon 教授（PI X 射线晶体学核心）实验室， 在结构性决定的司法研究工作中发挥并将继续发挥关键作用 他于 2001 年开始参与阿肯色州 INBRE，当时阿肯色州 BRIN 是第一个。 该项目由国家研究资源中心拨款资助，并由 Sakon 博士共同领导。 生物技术核心并在此角色中帮助阿肯色州 INBRE 网络教师和学生进入大学 阿肯色州核心设施的预算用于购买 HyPix-6000HE 混合光子计数。 用于 X 射线衍射系统的探测器和通用 Kappa 4 轴测角仪。 增强用户对新技术的访问，同时更换不再可用的过时设备 随着新安装、Saturn-92和AFC9的卸载，左侧的铍窗口。 该系统于 2003 年购买，已提供了良好的回报。 该设施中解决的 81 个结构经过验证并可从 17 年的投资中获取。 蛋白质数据库 (PDB)。该设施提供了我们州存放的 78.4% 的三维结构。 在 2003 年至 2021 年期间，坐标被频繁下载（1,685,267 次）并且 使用 PDB 实施的网站工具进行了检查（503,322 次），并在影响力方面领先我们的州（87.9% 下载和查看）。这些结构有助于理解蛋白质结构和设计新的蛋白质结构。 然而，系统的探测器/测角仪部分迫切需要升级。 制造商不再支持探测器 更换现有探测器/测角仪部分 系统将延长 X 射线衍射系统的使用寿命，并提高 X 射线衍射系统的功能和可用性 新一代 X 射线探测器将产生更高质量的数据和软件。 配备探测器/测角仪替代品将利用最近开发的先进的新算法 即使在相同的信噪比下，也能够提取更准确的信息。 投资将扩展该仪器的能力，不再是专门用于蛋白质晶体学的系统 通过拟议的升级，能够生成高质量的小分子分析。 该系统的成本低于新的小分子晶体学系统，该设施可以继续 满足 INBRE、COBRE 以及本校区其他 NIH 支持的研究人员的需求， 全州范围内。