METABOLIC ANALYSIS INSTRUMENTATION TO ENHANCE TARGETED THERAPEUTICS STUDIES

代谢分析仪器可增强靶向治疗研究

• 批准号: 10400414
• 负责人: IGOR B RONINSON
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-10 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400414
• 关键词: Administrative Supplement; Aging; Apoptotic; Area; Atrophic; Automobile Driving; Brain; Cancer Model; Cell Count; Cell Cycle; Cell Nucleus; Cellular Stress; Centers of Research Excellence; Data; Diabetes Mellitus; Disease; Drug Design; Flow Cytometry; Funding; Genetic; Genetic Transcription; Genus Hippocampus; Goals; HIV; Image; Implant; Infrastructure; Investigation; Knock-out; Laboratories; Longevity; Manufacturer Name; Measures; Mediator of activation protein; Metabolic; Metabolism; Microglia; Microscopy; Mitochondria; Modification; Molecular Target; Neurodegenerative Disorders; Neurons; Organelles; Oxidative Phosphorylation; Oxidative Stress; Peptides; Pharmaceutical Preparations; Pharmacology; Phosphotransferases; Play; Post-Translational Protein Processing; Process; Research; Research Personnel; Research Project Grants; Research Support; Resources; Rodent Model; South Carolina; System; Testing; Therapeutic; Therapeutic Studies; Universities; age related; base; cellular targeting; college; drug candidate; drug synthesis; equipment acquisition; experience; extracellular; flexibility; fluorescence imaging; functional genomics; imaging capabilities; instrument; instrumentation; metabolic abnormality assessment; multidisciplinary; nanoparticle; neurotransmission; novel therapeutics; response; scaffold; targeted treatment; therapeutic target; tissue regeneration

The COBRE Center for Targeted Therapeutics (CTT) at the University of South Carolina (UofSC) supports research that seeks to develop new and more effective classes of drugs against various diseases, by aiming these drugs at molecular and cellular targets that play a key role in the disease. Many therapeutic targets in different diseases either regulate or are functionally impacted by cellular metabolism. The ability to interrogate the metabolic effects of genetic or pharmacological modulation of such targets is critical to many CTT-affiliated investigators. The purpose of this proposal is to acquire an Agilent Seahorse Extracellular Flux (XF) Analyzer, including a module to facilitate data imaging and normalization. This will replace an older version of this instrument that cannot perform the high content imaging analyses and is no longer supported by the manufacturer. This instrument will belong to the Microscopy and Flow Cytometry Core of the CTT and will be overseen by a highly experienced investigator in cellular metabolic studies. There are several laboratories, including former and present CTT-funded investigators, which will be able to use the metabolic analysis instrument to increase their ability to achieve their research goals. Some of the projects by CTT-affiliated investigators that have an immediate need for the Seahorse Analyzer to investigate (i) the impact of metabolite derived protein modifications on neurodegenerative diseases (ii) metabolic effects of HIV-related changes in brain microglia, (iii) metabolic analysis of age-related changes in neurotransmission, (iii) effects of transcriptional Mediator kinase on cellular metabolism, (iv) cellular metabolic effects of nanoparticle and scaffold implants that deliver therapeutics to support tissue regeneration. Acquisition of this instrument will allow the continuation of these targeted therapeutics projects and will offer a more reliable way to use the data acquired. The capacity to measure cellular metabolism responses and adaptations will enhance the current CTT-supported projects and will allow CTT to extend its umbrella to other targeted therapeutics researchers whose research areas incorporate metabolic analyses.

南卡罗来纳大学（UOFSC）的靶向治疗中心（CTT）支持 旨在通过瞄准来开发针对各种疾病的新的，更有效的药物的研究 这些药物在分子和细胞靶标中起着关键作用的分子和细胞靶标。许多治疗目标 不同的疾病可以调节或在功能上受细胞代谢的影响。审问的能力 此类靶标的遗传或药理调节的代谢作用对于许多与CTT相关的 调查人员。该提案的目的是获得敏捷的海马细胞外通量（XF）分析仪， 包括一个促进数据成像和归一化的模块。这将替换一个较旧的版本 无法执行高内容成像分析的仪器，并且不再由 制造商。该仪器将属于CTT的显微镜和流式细胞仪核心，将是 由经验丰富的细胞代谢研究研究者监督。有几个实验室， 包括以前和现在的CTT资助的研究人员，该研究人员将能够使用代谢分析 提高实现研究目标能力的工具。 CTT附属的一些项目 立即需要海马分析仪来调查（i）代谢物的影响的研究人员 对神经退行性疾病的衍生蛋白质修饰（II）与HIV相关变化的代谢作用 脑小胶质细胞，（III）神经传递的年龄相关变化的代谢分析，（iii）转录的影响 介质激酶对细胞代谢，（IV）纳米颗粒和支架植入物的细胞代谢作用 提供治疗剂以支持组织再生。获取该工具将允许继续 这些有针对性的治疗项目，并将提供更可靠的方法来使用所获取的数据。能力 测量细胞代谢反应和适应将增强当前的CTT支持项目和 将使CTT将其保护伞扩展到其他有针对性的治疗研究人员，其研究领域 纳入代谢分析。