Integrative analyses of the kinetochore and the spindle assembly checkpoint

动粒和纺锤体装配检查点的综合分析

• 批准号: 10393295
• 负责人: Ajit Joglekar
• 金额: $ 5.97万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10393295
• 关键词: Academia; Award; Basic Science; Biochemical; Biophysics; Cancerous; Cell Cycle Regulation; Cell division; Cells; Cellular biology; Chromosomes; Code; Complex; Data Analyses; Dedications; Development; Ensure; Faculty; Financial Support; Funding; Future; Genome; Institution; Kinetochores; Learning; Mentorship; Methods; Microscopy; Mitosis; Molecular; Negative Staining; Parents; Phase; Polishes; Process; Protein Engineering; Proteins; Pythons; Research; Research Project Grants; Resource Development; Schools; Science; Scientist; System; Talents; Time; Universities; Work; base; career; daughter cell; experience; graduate student; insight; meetings; molecular imaging; pleasure; programs; recruit; single molecule

Abstract My lab combines cell biology and biophysics to conduct integrative analyses of two complex cellular systems: the kinetochore, a protein machine that moves chromosomes during mitosis, and the Spindle Assembly Checkpoint (SAC), which is a cell cycle control. Even as we are making excellent progress on the stated aims of our project, I have started developing the next phase of this research that involves replacing components of the kinetochore protein machine with de novo designed proteins. For this exciting work, I recruited Mr. Martin Fernandez, a recent graduate from the Lehigh University. Martin works in my lab as a research technician, spending 60% of his time on active research involving benchwork, data analysis, and presentation, and the remaining 40% of the time on lab management duties. I have had the pleasure of working with Martin for over 6 months, and this experience makes it clear to me that: (a) Martin possesses the talent and dedication to emerge as a professional in the biomedical sciences, and (b) Martin has a passion for basic research and wants to ultimately join the academia. Based on my experience, I am confident that he will successfully apply for joining a graduate program in the coming year. Therefore, I want to maximize the impact of Martin’s work in my lab over his career by ensuring that: (a) he dedicates 100% of his time to research, and (b) he gets the full benefit of the mentorship and development resources that I can provide. For this reason, I, together with Martin, am applying for a diversity supplement to cover Martin’s efforts for 1 year. During this year, Martin will work essentially as a new graduate student in my lab and drive an exciting research project forward. He will learn cutting-edge experimental methods (negative-stain EM microscopy, single molecule imaging, etc.). He will also engage in professional development activities (learning to code using Python, networking with graduate students and faculty at my institution, presenting his work at the ASCB meeting, putting together a polished application for graduate school, etc.). I am confident that Martin will fully utilize the financial support and recognition that comes with this funding and start his career as a scientist in the near future.

抽象的 我的实验室结合细胞生物学和生物物理学对两种复合物进行综合分析 细胞系统：动粒，一种在有丝分裂期间移动染色体的蛋白质机器， 以及主轴组装检查点（SAC），这是一个细胞周期控制。 我们项目的既定目标取得了巨大进展，我已经开始开发下一阶段 这项研究涉及从头更换动粒蛋白质机器的组件 为了这项令人兴奋的工作，我聘请了刚毕业的马丁·费尔南德斯先生。 马丁来自理海大学，在我的实验室担任研究技术员，花费了他 60% 的时间。 用于积极研究的时间，包括基准工作、数据分析和演示，其余时间 我有 40% 的时间从事实验室管理工作，很高兴与 Martin 一起工作。 超过 6 个月的时间，这段经历让我清楚地认识到： (a) 马丁拥有才华和 致力于成为生物医学领域的专业人士，并且 (b) 马丁充满热情 根据我的经验，我希望进行基础研究并最终加入学术界。 相信他能在来年成功申请加入研究生课程。 因此，我想通过确保马丁在我实验室的工作对他的职业生涯产生最大的影响 (a) 他将 100% 的时间投入到研究中，并且 (b) 他充分受益于研究 我可以提供指导和发展资源因此，我和马丁一起， 我正在申请多元化补助金，以支付马丁今年的努力。 基本上将作为我实验室的新研究生工作并推动一个令人兴奋的研究项目 他将学习尖端的实验方法（负染色电镜、单次染色）。 他还将从事专业发展活动（学习 使用 Python 编写代码，与我所在机构的研究生和教师建立联系，展示他的 在 ASCB 会议上工作，整理一份完善的研究生院申请等）。 相信马丁将充分利用由此带来的财务支持和认可 资助并在不久的将来开始他作为科学家的职业生涯。