Elucidating glial diversity in the peripheral nervous system

阐明周围神经系统中的神经胶质多样性

• 批准号: 10314960
• 负责人: Sarah C Kucenas
• 金额: $ 44.41万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10314960
• 关键词: Ablation; Axon; Candidate Disease Gene; Cell Nucleus; Cells; Cephalic; Clustered Regularly Interspaced Short Palindromic Repeats; Color; Coupled; Coupling; Data; Development; Disease; Distal; Dorsal; Event; Fertilization; Genes; Goals; Hour; Injury; Larva; Lasers; Lead; Light; Mediating; Modeling; Molecular; Motor; Nerve; Nervous system structure; Neural Crest Cell; Neuroglia; Nuclear; Organism; Perineuriums; Peripheral; Peripheral Nerves; Peripheral Nervous System; Pharmacology; Population; Population Dynamics; Predisposition; Process; Resources; Role; Schwann Cells; Sensory; Small Nuclear RNA; Specific qualifier value; Spinal; Spinal Cord; Structure; System; Taxonomy; Time; Transgenic Organisms; Zebrafish; cell type; genetic information; genetic signature; glial cell development; imaging genetics; in vivo; migration; mutant; nervous system development; novel; oligodendrocyte progenitor; stem cells; tool; transcriptome sequencing

Peripheral nerve assembly requires the coordinated development of many components, including axons and the glial cells that ensheath them. Currently, we have a broad perspective of the structure of peripheral nerves and the events that govern nerve assembly. However, we still lack a complete picture of all of the cellular and molecular mechanisms that mediate these events, and whether perturbations to these processes underlie disease. This is in part due to the fact that we still don’t have a full appreciation of the diversity of glial populations that make up these structures and there are currently no specific markers for any peripheral glial population. Additionally, although peripheral nerves are often grouped together as a single, uniform structure, there are in fact many types, including cranial, trunk, sensory, and motor, as well as spatial organization along the proximal-distal axis. However, whether there is any glial diversity between or along these structures, and whether this diversity is what contributes to the differences observed between these nerves with respect to function and susceptibility to disease, has yet to be determined. Using single-nuclei RNA-sequencing coupled with the powerful system, zebrafish, we will create a molecular taxonomy of peripheral glial subpopulations and decipher their roles in nervous system development.

周围神经组装需要许多组件的协调发展，包括轴突和 将其围成的神经胶质细胞。目前，我们对周围神经的结构有广泛的看法 管理神经集会的事件。但是，我们仍然缺乏所有细胞的完整图片 介导这些事件的分子机制，以及对这些过程的扰动是否构成 疾病。这部分是由于我们仍然对神经胶质种群的多样性不完全欣赏 构成了这些结构，目前尚无针对任何外围神经胶质种群的特定标记。 此外，尽管外周神经系统通常被分组为一个单一的均匀结构，但仍存在 事实，许多类型的类型，包括颅，后备，感觉和运动，以及沿近端轴的空间组织。但是，这些结构之间还是沿这些结构之间有任何神经胶质多样性，以及这是否存在 多样性是导致这些神经在功能和功能方面观察到的差异的原因 对疾病的敏感性尚未确定。使用单核RNA-Sere-Sequping与 强大的系统，斑马鱼，我们将创建一个分子分类，对外周神经胶质亚群和破译 它们在神经系统发展中的作用。

项目成果

Cd59 and inflammation regulate Schwann cell development.

• DOI: 10.7554/elife.76640
• 发表时间: 2022-06-24
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Wiltbank, Ashtyn T.;Steisnon, Emma R.;Criswell, Stacey J.;Piller, Melanie;Kucenas, Sarah
• 通讯作者: Kucenas, Sarah

Glutamate Signaling via the AMPAR Subunit GluR4 Regulates Oligodendrocyte Progenitor Cell Migration in the Developing Spinal Cord.

通过 AMPAR 亚基 GluR4 的谷氨酸信号传导调节发育中脊髓中的少突胶质细胞祖细胞迁移。

• DOI: 10.1523/jneurosci.2562-20.2021
• 发表时间: 2021
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Piller,Melanie;Werkman,IngeL;Brown,RobinIsadora;Latimer,AndrewJ;Kucenas,Sarah
• 通讯作者: Kucenas,Sarah