Center for Live Cell Genomics

活细胞基因组学中心

基本信息

批准号：
10307037
负责人：
DAVID H HAUSSLER
金额：
$ 250.64万
依托单位：
UNIVERSITY OF CALIFORNIA SANTA CRUZ
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-15 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10307037
关键词：
3-Dimensional 3D Print Address Area Biological Assay Biopsy Bioreactors Blood Blood Vessels Brain Brain Injuries CRISPR interference Cancer Patient Cell Culture Techniques Cell Line Cells Cellular Phone Cellular biology Cerebrum Childhood Glioma Clinical Data Clustered Regularly Interspaced Short Palindromic Repeats Coculture Techniques Code Communities Competence Complementary DNA Computer software Computers Data Data Analyses Data Set Development Devices Disadvantaged Disease Disease model Drug Screening Ecosystem Education and Outreach Educational workshop Electrodes Ensure Environment Evolution Feedback General Population Genes Genomic Data Commons Genomic approach Genomics Genotype Geometry Growth Human Hydrogels Image Immersion Incubators Individual Institution Intercellular Fluid International Internet Internet of Things Laboratories Link Machine Learning Maintenance Malignant Childhood Neoplasm Malignant Neoplasms Maps Membrane Potentials Mentorship Methodology Microfluidics Microscopy Modeling Molds Molecular Monitor Morphology National Human Genome Research Institute Neurobiology Neurosciences Research Operative Surgical Procedures Organoids Pathogenesis Pathway interactions Patients Pattern Pediatric Oncology Performance Perfusion Phenotype Pongidae Postdoctoral Fellow Proteins RNA Reporter Reproducibility Research Research Personnel Resolution Resources Schools Scientist Secondary Schools Speed Students System Techniques Technology Testing Therapeutic Tissue Model Tissues Training Training Programs Universities Venous Work anticancer research base cancer education cell type cohort community college cost data sharing design driver mutation engineered stem cells exosome experimental study flexibility genetic variant genome sciences genomic data glioma cell line graduate student improved innovation interest nanopore nervous system disorder neural circuit neurodevelopment new technology next generation novel strategies online community online resource open source optogenetics outreach pedagogy petabyte pre-clinical precision oncology programs remote control response scaffold science education screening sensor stem cells stroke model student training three dimensional cell culture tissue culture transcriptomics tumor undergraduate student webinar

项目摘要

ABSTRACT Center for Live Cell Genomics We will build new methodology and capacity for large-scale, long-term, inexpensive, modular, customizable, shared, Internet-of-Things-controlled, reproducible live cell culture and tissue-based experimental genomics disease models. Tissue models include traditional cell culture as well as organoid and primary tissue explants obtained from surgery or biopsy. Organoid factories supporting tissue growth and maintenance will be integrated with external and on-chip electro-optofluidic analytical modules to become part of an ecosystem that is modeled after open-source software. It will use commodity sensors, cameras, and computers linked in platforms flexibly designed using simple 3D printing, molding, etching and milling techniques potentially available at any institution. This will stimulate rapid innovation in experimental platforms for tissue culture. We will push this technology and use its best-in-class capabilities to make progress in neurodevelopment and pediatric cancer, addressing big questions. What genes contribute most importantly and specifically to human brain development? How do they go wrong in neurodevelopmental disease or brain injury? What specific molecular pathways are disrupted in individual pediatric cancer cases? How can we test pathway-specific treatments in a tissue model specific to each patient? Our education and outreach plans include a training program to develop a diverse and inclusive cohort of undergraduate students trained in genomic science through secondary school and community college outreach as well as coding workshops and research-based laboratory classes for UCSC undergraduates to develop core competencies. Participation in these activities serve as a basis for training graduate students and postdocs in inclusive pedagogy and mentorship. We are also developing a one-stop information hub to form an online community and to share our technology through immersive webinars and tutorials aimed at a broader audience with a range of expertise from the general public to scientists and clinicians at research institutions. Our work will enable significant advances in neuroscience and cancer research and education, stimulate a new open-source culture in cell biology and genomics, and democratize scientific and educational access beyond elite institutions, extending sharing projects like NHGRI ANvIL beyond data and code to include experiments and Internet-connected experimental platforms.

抽象的活细胞基因组学中心我们将建立新的方法和能力，用于大规模，长期，廉价，模块化，可自定义的，共享的，通过互联网控制的，可再现的活细胞培养和基于组织的活细胞培养实验基因组疾病模型。组织模型包括传统的细胞培养以及从手术或活检获得的器官和原发性组织外植体。提供支持的器官工厂组织生长和维护将与外部和芯片电流荧光分析集成模块成为以开源软件建模的生态系统的一部分。它将使用使用简单3D灵活设计的平台上链接的商品传感器，相机和计算机任何机构都可以使用印刷，成型，蚀刻和铣削技术。这会在组织培养的实验平台中刺激快速创新。我们将推动这项技术，并利用其一流的能力来取得进展神经发育和小儿癌，解决了大问题。什么基因最大的贡献重要的是专门针对人类脑发育？他们如何出错神经发育疾病或脑损伤？哪些特定的分子途径被破坏个体儿科癌症病例？我们如何在组织模型中测试途径特异性治疗特定于每个患者？我们的教育和外展计划包括一项培训计划，以开发多样化和包容的队列通过中学和社区接受基因组科学培训的本科生大学外展以及UCSC的编码研讨会和基于研究的实验室课程大学生以发展核心能力。参加这些活动是培训研究生和博士后，包括教学法和指导。我们也在发展一个一站式信息中心，以组建在线社区并通过身临其境的网络研讨会和教程针对更广泛的受众，具有一系列专业知识研究机构的科学家和临床医生的公众。我们的工作将在神经科学和癌症研究和教育方面取得重大进步，刺激细胞生物学和基因组学中新的开源文化，使科学化和民主化超越精英机构的教育访问，将NHGRI Anvil等共享项目扩展到数据之外和代码，包括实验和与Internet连接的实验平台。