Neural sequences for planning and production of learned vocalizations

用于规划和产生学习发声的神经序列

• 批准号: 10227577
• 负责人: Brenton G. Cooper
• 金额: $ 1.28万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10227577
• 关键词: Acute; Architecture; BRAIN initiative; Behavior; Biological Models; Birds; Brain; Calcium; Cells; Complex; Electrophysiology (science); Foundations; Goals; Image; Letters; Methods; Monitor; Motor; Motor Skills; Names; Neurons; Optics; Performance; Preparation; Production; Research; Research Support; Research Training; Schools; Scientist; Songbirds; Structure; Synapses; Testing; Training; bird song; cell type; graduate student; insight; novel; optogenetics; parent grant; relating to nervous system; vocalization

PROJECT SUMMARY Sequences of neuronal activity are thought to underlie planning, preparation, and production of voluntary skilled behaviors. Dissecting how these premotor and motor sequences are functionally and synaptically integrated to support fluent, context-appropriate performance of natural behaviors is a major research challenge and a central goal of the BRAIN Initiative. The songbird premotor cortical structure HVC (letters used as proper name) has emerged as a prominent model system for studying how sparse neural sequences underlie the production of a precise, ethologically relevant behavior - birdsong. A single class of projection neurons in HVC is necessary for acute performance of birdsong. Yet, technical limitations associated with cell- type selective monitoring and manipulation of these neurons has hindered the ability to study how their neural sequences are functionally and synaptically integrated to support the planning, preparation and execution of behavior. This Diversity Supplement seeks to support the research and training of a new graduate student, Carlos Orozco, who will be working on aims 3 and 4 of the parent grant R01NS108424 (Neural sequences for planning and production of learned vocalizations). Mr. Orozco will be trainined in using calcium imaging, electrophysiological recordings and optogenetic manipulations in freely singing birds to test how diverse neural sequences in HVC underlie the planning, preparation and production of song. In addition, he will use single-cell optogenetics, calcium imaging, and circuit mapping methods to test the functional, synaptic and areal organization of this circuit. Drawing on training in a variety of cutting-edge approaches, this diversity Supplement aims to provide foundational training to a young scientist embarking on graduate school and provide insights into how diverse neural sequences are synaptically integrated to support fluent production of a voluntary skilled behavior.

项目概要 神经元活动的序列被认为是计划、准备和产生自愿性行为的基础。 熟练的行为。剖析这些前运动序列和运动序列的功能和突触功能 整合以支持自然行为的流畅、适合情境的表现是一项重大研究 挑战和 BRAIN 计划的中心目标。鸣禽前运动皮层结构 HVC（使用的字母 作为专有名称）已成为研究稀疏神经序列的重要模型系统 是产生精确的、与行为学相关的行为——鸟鸣的基础。单类投影 HVC 中的神经元对于鸟鸣的敏锐表现是必需的。然而，与细胞相关的技术限制 对这些神经元的类型选择性监测和操纵阻碍了研究它们的神经元如何运作的能力 序列在功能上和突触上进行整合，以支持计划、准备和执​​行 行为。该多样性补充材料旨在支持新研究生的研究和培训， Carlos Orozco，他将致力于母基金 R01NS108424（神经序列 学习发声的规划和制作）。奥罗斯科先生将接受钙成像使用培训， 对自由歌唱的鸟类进行电生理记录和光遗传学操作，以测试神经元的多样性 HVC 中的序列是歌曲的规划、准备和制作的基础。此外，他还将使用单细胞 光遗传学、钙成像和电路映射方法来测试功能、突触和区域 该电路的组织。借鉴各种尖端方法的培训，这种多样性 增刊旨在为即将进入研究生院的年轻科学家提供基础培训， 深入了解不同的神经序列如何突触整合以支持流畅的生产 自愿的熟练行为。