WaNPRC Macaca nemestrina SPF Breeding Colony

WaNPRC 猕猴 SPF 育种群

• 批准号: 10203135
• 负责人: CHARLOTTE HOTCHKISS
• 金额: $ 57.19万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10203135
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Adverse event; Alleles; Anatomy; Animal Genetics; Animal Model; Animals; Area; Arizona; Behavioral; Biomedical Research; Breeding; Characteristics; Chickenpox; Communication; Communities; Complex; Cytomegalovirus; Diagnostic Services; Ensure; Epithelial; Epithelium; Exclusion; Exhibits; Exposure to; Faculty; Future; Genetic; Genomics; Goals; Grant; Guidelines; Health; Human; Immune response; Immunologics; Infant; Infection; Infrastructure; Laboratories; Laboratory Research; Location; Macaca; Macaca mulatta; Macaca nemestrina; Maintenance; Mission; Modeling; Pathogenicity; Physiological; Primates; Production; Productivity; Research; Research Personnel; Resources; Rhadinovirus; Risk; SIV; Sales; Simian Foamy Virus; Simian T-lymphotropic virus 1; Site; Source; Subfamily lentivirinae; Testing; United States; United States National Institutes of Health; Vagina; Viral; Virus; Washington; animal breeding; animal care; base; bioinformatics tool; cost; cost effectiveness; germ free condition; human disease; human model; improved; innovation; keratinization; microbicide; microorganism; nonhuman primate; pathogen; pre-exposure prophylaxis; vaginal microbiome; virology

Summary: The goal of this project is to maintain and enhance the specific pathogen free (SPF) pigtail macaque (M. nemestrina) breeding colony at the Washington National Primate Research Center (WaNPRC). This colony is the only major domestic breeding colony of M. nemestrina and the primary source of this important animal model for AIDS studies and other types of biomedical research in the United States. M. nemestrina have unique immunological, genetic, behavioral, anatomical, and physiological characteristics that make them an essential model in a number of areas of research related to HIV/AIDS. Specific pathogens (SIV, SRV, STLV-1, McHV-1) are a threat to animal or human health, or interfere with research and must be excluded to optimize nonhuman primate research models. In this proposal we discuss how we will maintain the WaNPRC SPF M. nemestrina breeding colony to provide animals of the highest quality to meet research needs. The SPF colony is housed at two locations, the Arizona Breeding Colony (ABC) which is an integral part of the WaNPRC, and at the New Iberia Research Center (NIRC). Having animals at two sites raises some challenges, but provides for contingencies in case of adverse events, and we have established lines of communication to ensure consistency. At both sites we will use innovative bioinformatics tools to increase cost effectiveness by maximizing production per animal. In addition, in our Seattle Infant Primate Research Laboratory (IPRL) we will initiate breeding for the production of expanded SPF M. nemestrina, which will be free of simian foamy virus (SFV), cytomegalovirus (MnCMV), rhadinovirus (MnRV), and simian varicella (SVV). Viral exclusion testing for all present and future excluded viruses will be performed by the Primate Diagnostic Services Laboratory (PDSL) through the virology core. We will use state-of-the-art genomics to characterize the animals, focusing on expressed alleles in the MHC region through the MHC Genetic Typing Core.

概括： 该项目的目标是维持和增强无特定病原体（SPF）猪尾猕猴（M. nemestrina）在华盛顿国家灵长类研究中心（WaNPRC）的繁殖群。这个殖民地是 M. nemestrina 唯一的主要国内繁殖地，也是这种重要动物的主要来源 美国艾滋病研究和其他类型生物医学研究的模型。 M. nemestrina 有 独特的免疫学、遗传、行为、解剖和生理特征，使它们成为 与艾滋病毒/艾滋病相关的许多研究领域的基本模型。特定病原体（SIV、SRV、STLV-1、 McHV-1）对动物或人类健康构成威胁，或干扰研究，必须排除以优化 非人类灵长类动物研究模型。在本提案中，我们讨论如何维护 WaNPRC SPF M。 Nemestrina 繁殖群提供最高品质的动物以满足研究需求。 SPF 菌落 位于两个地点：亚利桑那育种场 (ABC)，它是 WaNPRC 的组成部分，以及 在新伊比利亚研究中心（NIRC）。在两个地点饲养动物会带来一些挑战，但也提供了 对于发生不良事件时的意外情况，我们建立了沟通渠道以确保 一致性。在这两个地点，我们将使用创新的生物信息学工具来提高成本效益 最大化每只动物的产量。此外，在我们的西雅图婴儿灵长类动物研究实验室 (IPRL)，我们 将开始育种以生产扩大的 SPF M. nemestrina，该产品将不含猿猴泡沫 病毒（SFV）、巨细胞病毒（MnCMV）、鼻病毒（MnRV）和猿水痘病毒（SVV）。病毒排除 所有当前和未来排除的病毒的测试将由灵长类动物诊断服务进行 实验室（PDSL）通过病毒学核心。我们将使用最先进的基因组学来表征 动物，通过 MHC 遗传分型核心重点关注 MHC 区域中表达的等位基因。