Employing biofilters to detect human pathogens in Mid-Atlantic US waters

使用生物过滤器检测美国大西洋中部水域的人类病原体

• 批准号: 10196293
• 负责人: Katrina Lohan
• 金额: $ 6.73万
• 依托单位: SMITHSONIAN INSTITUTION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-21 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10196293
• 关键词: Comparative Study; Consumption; Data; Databases; Development; Disease; Disease Outbreaks; Ecology; Environment; Environmental Epidemiology; Epidemiology; Farming environment; Future; Genetic; Genome; Genomic Segment; Genomics; Harvest; Health; Human; Individual; Knowledge; Methodology; Methods; Molecular; Organism; Oysters; Parasites; Pathogenicity; Plants; Population; Public Health; Publishing; Research; Resolution; Resources; Safety; Sampling; Seafood; Sensitivity and Specificity; Source; Spatial Distribution; Testing; Tissues; United States; Viral; Water; coastal water; design; epidemiology study; food consumption; food resource; human pathogen; molecular array; novel; pathogen; pathogenic bacteria; pathogenic virus; tool; transmission process; water quality

PROJECT SUMMARY Throughout the United States, human populations in coastal zones have been steadily increasing. Additionally, humans partake in many aquatic recreational activities and consume significant amounts of seafood either farmed or harvested from coastal waters. All of these activities increase the diversity, dispersal, and transmission potential of human pathogens. Unfortunately, knowledge of the environmental epidemiology of human pathogens in coastal waters is limited. Tools capable of generating high-resolution information are needed to determine the sources, connectivity, and spatial distribution of these organisms in coastal environments. This project proposes the design, optimization, and utilization of a set of in-solution sequence capture arrays to conduct targeted enrichment of viral and bacterial human pathogens to determine their diversity, distribution, dispersal, and connectivity in coastal waterways. Both capture arrays will utilize existing genomic resources to create the arrays, which will include a mixture of shared and unique loci. Using a variety of conserved and variable loci from these pathogens allows us to capture genomic regions useful for comparative studies across taxa (shared loci) and for population genomic studies (unique loci) within taxa. The efficiency, sensitivity, and specificity of the designed capture arrays will be assessed by testing these arrays on samples from biofilters, which will be market-sized oysters deployed downstream from wastewater treatment facilities. The data generated from this project will be used to conduct preliminary environmental molecular epidemiological studies on these parasite taxa across spatial and temporal scales. This information is critical for public health officials to make informed decisions about the health and safety of humans using, living near, and consuming food resources from coastal waters.

项目摘要 在整个美国，沿海地区的人口一直在稳步增加。 此外，人类参加了许多水上娱乐活动，并消费大量 从沿海水域耕种或收获的海鲜。所有这些活动都增加了多样性， 人类病原体的分散和传播潜力。不幸的是，了解 沿海水域中人类病原体的环境流行病学有限。能够的工具 需要生成高分辨率信息来确定来源，连通性和空间 这些生物在沿海环境中的分布。该项目提出了设计，优化， 并利用一组实体序列捕获阵列以进行靶向富集病毒和 细菌性人类病原体以确定其多样性，分布，分散和连通性 沿海水道。两个捕获数组都将利用现有的基因组资源来创建数组， 其中包括共享和独特的基因座的混合物。使用各种保守和可变基因座 从这些病原体中，我们可以捕获对跨类群比较研究有用的基因组区域 （共享基因座）和人群基因组研究（独特的基因座）。效率，灵敏度， 设计捕获阵列的特异性将通过测试这些阵列的样品来评估 生物过滤器将是从废水处理下下游部署的市场大小的牡蛎 设施。该项目产生的数据将用于进行初步环境 关于这些寄生虫分类群的分子流行病学研究跨空间和时间尺度。这 信息对于公共卫生官员必须就健康和安全做出明智的决定至关重要 人类使用，在附近生活并从沿海水域消费食物资源。