ShEEP Request for Confocal Microscope

ShEEP 请求共焦显微镜

• 批准号: 10179606
• 负责人: Machelle T. Pardue
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10179606
• 关键词: 12 year old; Alzheimer' s Disease; Area; Artificial Intelligence; Biological; Cells; Cerebrovascular system; Computer Workstations; Confocal Microscopy; Data; Diabetes Mellitus; Disease; Electrophysiology (science); Eye; Fibronectins; Funding; Future; Glaucoma; Goals; Health; Healthcare; Healthcare Systems; Image; Infection; Injury; Label; Light; Lung; Malignant Neoplasms; Microscope; Minor; Muscle; Neurons; Noise; Optics; Organ; Peripheral arterial disease; Photobleaching; Pulmonary Hypertension; Research; Research Personnel; Resolution; Sampling; Scanning; Scientist; Sheep; Signal Transduction; Skin; Source; Speed; Stroke; Structure; Subcellular structure; System; Time; Tissue imaging; Tissues; Traumatic Brain Injury; Veterans; base; bone; confocal imaging; high end computer; imaging software; improved; inflammatory disease of the intestine; innovation; lens; live cell imaging; pathogen; reconstruction; square foot; three dimensional structure; tool; ventilation

Abstract Microscopes were first invented in the 1590s and quickly became a fundamental part of research to visualize tissues and cells. In the 1940s, confocal microscopy was first introduced to increase optical resolution. The ability to image tissue at different depths enabled the reconstruction of three-dimensional structures within an object which greatly aided in understanding the relationship between different intracellular structures as well as the structural relationship between cells and neurons in healthy and diseased tissues. Due to this ability to interrogate biological structures in such detail, confocal microscopy has become an essential tool for scientists. Here, we request a new Nikon A1R HD25 MP ready confocal microscope which will replace our existing Olympus FV1000 confocal microscope that is over 12 years old (obtained with ShEEP funds in 2008). The Nikon A1R confocal has multiple advancements, including higher resolution, larger imaging area, faster acquisition speed, increased analytical throughput, more sophisticated imaging software that includes artificial intelligence components, live cell imaging and the ability to perform electrophysiology recordings simultaneously. Importantly, this new Nikon A1R confocal microscope can be easily upgraded to include multiphoton capabilities in the future, using the same footprint. Eighteen investigators at the Atlanta VA Healthcare System were identified that would be major and minor users of this microscope. They were eager to support this proposal with anticipation that upgrading to the Nikon A1R confocal microscopy would enhance their research by providing advanced imaging options. Thus, we propose the purchase of the Nikon A1R confocal microscopy system to support and accelerate the innovative research being performed by Atlanta VA investigators to improve veteran healthcare.

抽象的 显微镜首先是在1590年代发明的，并迅速成为研究的基本组成部分 组织和细胞。在1940年代，首先引入共聚焦显微镜以增加光学分辨率。这 能够在不同深度成像组织的能力能够重建三维结构 对象极大地帮助理解不同细胞内结构之间的关系 健康和患病组织中细胞与神经元之间的结构关系。由于这种能力 询问生物结构的详细介绍，共聚焦显微镜已成为科学家的重要工具。 在这里，我们请求新的Nikon A1R HD25 MP Ready共聚焦显微镜，该显微镜将取代我们现有的 超过12年的Olympus FV1000共聚焦显微镜（2008年绵羊基金获得）。这 Nikon A1R共共聚焦有多个进步，包括更高的分辨率，较大的成像区域，更快的速度 采集速度，提高分析吞吐量，更复杂的成像软件，包括人造 智能成分，活细胞成像和执行电生理记录的能力 同时地。重要的是，这种新的尼康A1R共聚焦显微镜可以轻松升级到包括 将来使用相同的占地面积的多光子功能。弗吉尼亚州亚特兰大的十八位调查员 确定医疗保健系统将是该显微镜的主要和次要使用者。他们渴望 为了支持这项建议，预计将升级到尼康A1R共聚焦显微镜将增强 他们的研究通过提供高级成像选项。因此，我们建议购买尼康A1R 共聚焦显微镜系统支持和加速亚特兰大VA正在进行的创新研究 调查人员改善资深医疗保健。