ShEEP Request for ImageXpress Micro Confocal High Content Screening System

ShEEP 请求 ImageXpress Micro 共焦高内涵筛选系统

• 批准号: 10177122
• 负责人: James Michael Fleckenstein
• 金额: --
• 依托单位: ST. LOUIS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177122
• 关键词: 3-Dimensional; 4D Imaging; Adopted; Basic Science; Biological Models; Blood; California; Cancer Biology; Cardiology; Cells; Chemotherapy-Oncologic Procedure; Communicable Diseases; Complex; Computer software; Confocal Microscopy; Devices; Disease; Disease Management; Equipment; Fluorescence; Four-dimensional; Funding; Goals; Health; Healthcare Systems; Image; Immersion; In Vitro; Individual; Investigation; Laboratories; Lasers; Light; Manufacturer Name; Microscope; Molecular; Nature; Nephrology; Noise; Optics; Organ; Organoids; Pathogenesis; Patients; Penetration; Phase; Population Heterogeneity; Predisposition; Prevention strategy; Principal Investigator; Program Evaluation; Reporter; Research; Research Personnel; Resolution; Rheumatology; Saints; Sampling; Scientist; Signal Transduction; Source; Speed; System; Testing; Thick; Time; Tissues; Translations; Universities; Veterans; Washington; Water; Whole Organism; adult stem cell; cellular imaging; chemotherapeutic agent; computerized data processing; confocal imaging; contrast imaging; design; drug testing; high resolution imaging; high throughput screening; human tissue; image processing; imaging capabilities; imaging system; instrument; instrumentation; live cell imaging; medical schools; microbial; novel therapeutics; novel vaccines; personalized approach; programs; screening; stem cells; therapy development; tool; welfare

Project Summary/Abstract The goal of this ORD Shared Equipment Evaluation Program (ShEEP) proposal from the Saint Louis VA Healthcare System is to obtain a high content confocal microscopy imaging system capable of obtaining and processing high resolution images of multiple samples, including live cells. The system will be shared by multiple VA-funded investigators and their colleagues at two affiliated universities Saint Louis University School of Medicine and Washington University School of Medicine. Both affiliates have substantial research enterprises connected to the Saint Louis VA. The instrument is designed to support and enhance the research endeavors of a large and diverse group of VA-funded scientists. The instrumentation requested, is a Molecular Devices ImageXpress Micro Confocal High-Content Imaging System and accompanying analysis software that includes multiple application modules pre- loaded onto an analysis workstation designed to support the imaging and data processing needs for a wide variety of investigations. The proposed system is configured for spinning disc laser confocal microscopy to accommodate rapid imaging at multiple fluorescence wavelengths, water immersion objectives that permit increased depth of penetration for thicker samples at reduced exposure times, environmental control for imaging of live samples and phase contrast for imaging of unstained cells. In addition to the need for high content imaging and analysis, a common theme among the investigators supporting this proposal is the need to image and analyze organoids. These adult stem cell-derived clusters of cells recapitulate many of the features from the tissue from which they were obtained and therefore have now been adopted in multiple lines of investigation from personalized cancer chemotherapy to microbial pathogenesis studies. Multiple projects in this proposal relating cancer biology, rheumatology, nephrology, cardiology and infectious diseases are designed around the imaging of organoids as a platform for basic discovery and translation to therapies that would benefit our patients at the VA.

项目摘要/摘要 该ORD共享设备评估计划（SHEEP）提案的目标 VA Healthcare系统将获得能够的高素质共聚焦显微镜成像系统 获取和处理包括活细胞在内的多个样品的高分辨率图像。系统 多个由VA资助的调查员及其同事在两所关联大学中分享 圣路易斯大学医学院和华盛顿大学医学院。两个会员 拥有与圣路易斯弗吉尼亚州有关的大量研究企业。该仪器设计为 支持并增强了一大批由VA资助的科学家组的研究努力。 所请求的仪器是分子设备Imagexpress微共聚焦高质量 成像系统和随附的分析软件，其中包括多个应用程序模块 加载到分析工作站上，旨在支持成像和数据处理需求 各种各样的调查。提出的系统已配置用于旋转光盘激光共焦 显微镜以适应多种荧光波长的快速成像，浸入水 允许在减少暴露时间的较厚样品中增加渗透深度的目标， 用于成像的活样品成像和未染色细胞成像的相位对比度。 除了需要高内容成像和分析的需要之外，这是一个共同的主题 支持该建议的研究人员需要形象和分析类器官。这些成年茎 细胞来源的细胞簇概括了从其组织中的许多特征 获得并因此已在个性化的多种调查中采用 癌症化学疗法对微生物发病机理研究。此提案中的多个项目 癌症生物学，风湿病学，肾脏病，心脏病学和传染病是围绕围绕的 类器官的成像作为基本发现和转化为疗法的平台，将受益 我们在VA的患者。