Kinetic Imaging Cytometer (KIC) for High Throughput Studies of Cellular Physiology

用于细胞生理学高通量研究的动态成像细胞仪 (KIC)

• 批准号: 10175806
• 负责人: MARK MERCOLA
• 金额: $ 52.83万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-26 至 2022-10-25
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10175806
• 关键词: Biomedical Engineering; Cardiac Myocytes; Cardiovascular Diseases; Cardiovascular system; Cell physiology; Cells; Complications of Diabetes Mellitus; Development; Disease; Disease model; Electrophysiology (science); Ensure; Faculty; Faculty Recruitment; Funding; Goals; Grant; Heart failure; Image; Institutes; Institution; Kinetics; Laboratories; Maintenance; Medicine; Minor; Ophthalmology; Patient Care; Pediatrics; Physiological; Physiology; Play; Proteome; Research; Research Personnel; Resources; Robotics; Role; Science; Speed; Strategic Planning; System; Therapeutic; Time; United States National Institutes of Health; base; cancer therapy; drug development; experience; heart cell; improved; induced pluripotent stem cell; innovation; instrument; instrumentation; medical schools; operation; patch clamp; screening; senior faculty; stem cell model; whole genome

PROJECT SUMMARY/ABSTRACT: The Stanford Cardiovascular Institute (CVI) proposes the acquisition of a Vala Sciences’ IC200 Kinetic Imaging Cytometer (KIC) to be placed in a shared setting in the new Biomedical Innovations (BMI) Building that is centrally located on campus. The IC200 KIC is a cutting edge instrument that combines aspects of high content screening systems with high speed video imaging and cell-by-cell analysis features of physiological recording instrumentation such as patch clamp electrophysiology setups. No other type of instrument combines these features, and no comparable instrument is available on Stanford campus or in the vicinity. The instrument will be used for in-depth studies that probe disease mechanisms of excitable cells such as cardiomyocytes on an unprecedented whole genome and whole proteome level. The focus on mechanism-based understanding of disease, and ultimately the development of mechanism- based therapeutics, aligns with the long-term strategic plan of the Stanford CVI and School of Medicine, and is consistent with recent faculty recruitment. The capability of high throughput assessment of physiological function of heart cells plays a central role in this plan. The instrument will be managed by an experienced group with expertise in high content screening and disease modelling using induced pluripotent stem cell (iPSC)-based models. The CVI comprises more than 400 investigators and more than 125 faculty-led research groups and spans diverse departments including Medicine, Pediatrics, Ophthalmology and Biomedical Engineering. The Major and Minor Users highlighted in this proposal range from junior faculty who are just starting their own laboratories to established full professors with over 20 years of research experience. All the major users have NIH funding, and the collective benefit to NIH grants comprises over 80% of the anticipated user time of the instrument. The institution is committing considerable financial resources and dedicated space to ensure the maintenance and continued operation of the instrument, including its integration into a robotics facility. We expect that the IC200 will positively impact a broad cross section of cardiovascular and other diseases, notably heart failure and cardiovascular complications of diabetes and cancer treatments. In addition, the high throughput and physiology recording aspects of the IC200 will propel research towards our drug development and our translational goal of improving care of patients with cardiovascular disease.

项目摘要/摘要： 斯坦福心血管研究所（CVI）提议收购Vala Sciences的IC200 动力学成像细胞仪（KIC）将放置在新的生物医学中的共享环境中 创新（BMI）建筑物位于校园中心。 IC200 KIC是最前沿 将高内容筛选系统与高速视频相结合的仪器 物理记录仪器的成像和细胞分析特征，例如 斑块夹电生理设置。没有其他类型的乐器结合了这些功能，并且 斯坦福校园或附近没有可比的乐器。乐器将 用于深入研究，以探测令人兴奋的细胞的疾病机制，例如 在空前的整个基因组和整个蛋白质组水平上的心肌细胞。专注于 基于机制的理解疾病，最终是机制的发展 - 基于治疗剂，与斯坦福大学CVI和学校的长期战略计划保持一致 医学，与最近的教师招聘一致。高通量的能力 心脏细胞身体功能的评估在该计划中起着核心作用。乐器 将由一个经验丰富的小组管理，具有高素质筛查和疾病的专业知识 使用诱导多能干细胞（IPSC）的模型进行建模。 CVI包括更多 超过400名调查人员和125多名教师领导的研究小组和跨越多样 包括医学，儿科，眼科和生物医学工程等部门。这 在此提案中突出显示的主要用户和未成年人范围从刚开始的初级教师 他们自己的实验室为建立了20多年的研究经验的完整教授。 所有主要用户都有NIH资金，而NIH赠款的集体利益包括 该仪器的预期用户时间中有80％。该机构正在考虑 财政资源和专用空间，以确保维护和继续运营 该仪器，包括集成到机器人设施中。我们希望IC200将 积极影响心血管和其他疾病的广泛横截面，特别是心力衰竭 糖尿病和癌症治疗的心血管并发症。此外，高 IC200的吞吐量和生理记录方面将向我们的药物进行研究 发展和我们改善心血管疾病患者护理的转化目标。