Zeiss Lightsheet Upgrade to Expand Imaging Capabilities

蔡司 Lightsheet 升级以扩展成像能力

• 批准号: 10176288
• 负责人: Lisa A. Cameron
• 金额: $ 16.35万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10176288
• 关键词: 3-Dimensional; Basic Science; Biological; Brain; Cellular biology; Computer software; Core Facility; Developmental Biology; Disease; Disease model; Embryo; Equipment; Funding; Human; Image; Institution; Microscopy; Minor; Mus; Neurosciences; Optics; Organ; Preparation; Refractive Indices; Research; Research Personnel; Sampling; System; United States National Institutes of Health; Universities; base; experience; human disease; imaging capabilities; instrument; light microscopy; medical schools; operation

ABSTRACT Lightsheet microscopy offers distinct capability advantages for the imaging of live intact biological samples and optically cleared fixed samples. Rapid 3D optical sectioning with little photodamage enables lightsheet systems to excel at the imaging of large organs or whole embryos. The Zeiss Lightsheet Z.1 upgrades we propose in this application for higher refractive index clearing solutions, larger sample chambers and longer working distance, larger field of view objectives will transform researchers’ capabilities to collect images of larger samples such as cleared whole mouse brain or cleared whole mouse embryos. The availability of the upgrade hardware and software for our Zeiss lightsheet system would enhance many projects investigating fundamental developmental biology and human disease models. The current instrument is housed in a Duke School of Medicine building within the Light Microscopy Core Facility space. The upgrade parts would be installed onto to the Zeiss system which has been in operation for 3.5 years. In addition to the major and minor users’ research described here, the system and the upgrades would serve the entire research base at Duke University and Medical School where the impact and use would be widespread. The user group has experience with lightsheet imaging and sample preparation for the Zeiss system at Duke, along with other forms located at other institutions. The general high-levels of imaging expertise and specific experience with the Zeiss lightsheet are well placed to immediately and effectively implement this upgrade equipment to expand the Zeiss lightsheet system for an important array of basic research. These upgrades proposed will significantly contribute to new research capabilities related to NIH funded developmental biology, neuroscience, and cell biology questions relevant to human disorders as outlined in the research statement.

抽象的 Lightsheet显微镜为现场成像提供了不同的功能优势 生物样品和光学清除的固定样品。快速3D光学分段很少 光损伤使灯表系统能够在大型器官或整个器官的成像上表现出色 胚胎。 Zeiss Lightsheet Z.1我们在此应用程序中提出的升级升级，以进行更高的折射率 索引清除解决方案，较大的样品室和更长的工作距离，较大的场 查看目标将改变研究人员收集大型样本图像的能力 如清除的整个小鼠大脑或清除整个小鼠胚胎。升级的可用性 我们的Zeiss Lightsheet系统的硬件和软件将增强许多项目 研究基本发育生物学和人类疾病模型。电流 乐器位于光学显微镜核心内的杜克大学医学院大楼中 设施空间。升级零件将安装到已经进入的Zeiss系统上 运营3。5年。除了这里描述的主要和次要用户的研究外， 系统和升级将为杜克大学和医学的整个研究基础提供服务 影响和使用的学校将是广泛的。用户组有经验 杜克大学的蔡司系统的灯光片成像和样品制备以及其他形式 位于其他机构。成像专业知识和特定成像的一般高层 Zeiss Lightsheet的经验非常适合立即有效实施 此升级设备以扩展Zeiss Lightsheet系统，以提供一系列重要的基本阵列 研究。提出的这些升级将大大促进新的研究能力 与NIH基本发育生物学，神经科学和细胞生物学问题有关 如研究声明中概述的人类疾病。