timsTOF Pro Mass Spectrometer

timsTOF Pro 质谱仪

• 批准号: 10173053
• 负责人: RADOSLAV GOLDMAN
• 金额: $ 60万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10173053
• 关键词: Area; Behavior; Clinical; Data Analyses; Detection; District of Columbia; Funding; Glycopeptides; Glycoproteins; Laboratories; Mass Spectrum Analysis; National Center for Research Resources; Peptides; Proteins; Research; Research Personnel; Resolution; Services; Speed; Universities; analytical method; glycoproteomics; ion mobility; mass analyzer; mass spectrometer; tool

ABSTRACT Analysis of glycoproteins is a specialized field of protein mass spectrometry. Behavior of peptides differs substantially from the behavior of glycosylated peptides whose analysis requires adjusted analytical methods. Few laboratories provide services in glycoproteomics and glycoprotein characterization because of the need for adjustments of the analytical technics and data interpretation tools. Researchers at the Clinical and Translational Glycoscience Research Center (CTGRC) at Georgetown University have documented expertise in analytical glycoproteomics and the CTGRC serves as a major hub for glycoproteomics in the Washington DC area. Here we request funds for a timsTOF Pro mass spectrometer to enable glycoproteomic projects supported by the CTGRC. This mass spectrometer has an exceptional combination of trapped ion mobility with high speed and resolution QTOF mass analyzer. The newly optimized workflows for efficient separation and fragmentation of glycopeptides on the timsTOF Pro allow unprecedented sensitivity in the detection, resolution and quantification of glycopeptides. The support from NCRR will enable us to provide expert support, currently in high demand and short supply, to funded projects at Georgetown University, in the Washington DC area, and at the National level.

抽象的 糖蛋白的分析是蛋白质质谱法的专业领域。肽的行为有所不同 从基本上来自分析需要调整的分析方法的糖基化肽的行为。 很少有实验室提供糖蛋白质组学和糖蛋白特征的服务，因为 用于调整分析技术和数据解释工具。临床研究人员 乔治敦大学的转化性聚糖研究中心（CTGRC）已记录了专业知识 在分析性糖蛋白质组学和CTGRC中是华盛顿糖蛋白质组学的主要枢纽 DC区域。在这里，我们要求资金用于Pro质谱仪，以启用糖蛋白质组学项目 由CTGRC支持。该质谱仪具有捕获的离子迁移率与 高速和分辨率QTOF质量分析仪。新优化的工作流程，以进行有效的分离和 在timstof Pro上的糖肽的碎片使检测中的敏感性在分辨率中允许前所未有的灵敏度 和糖肽的定量。 NCRR的支持将使我们能够提供专家支持，目前 在高需求和供不应求的情况下，在华盛顿特区乔治敦大学的资助项目中， 并在国家一级。