5600 TripleTOF Mass Spectrometry

5600 TripleTOF 质谱仪

• 批准号: 8448387
• 负责人: RADOSLAV GOLDMAN
• 金额: $ 38.13万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-15 至 2014-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448387
• 关键词: Academic Medical Centers; Area; Climate; Communities; Complex; Comprehensive Cancer Center; Development; District of Columbia; Economics; Evaluation; Funding; Laboratories; Maintenance; Mass Spectrum Analysis; NCI-Designated Cancer Center; National Center for Research Resources; Performance; Post-Translational Protein Processing; Protein Analysis; Protein Glycosylation; Proteome; Proteomics; Request for Proposals; Research; Research Project Grants; Resource Sharing; Resources; Sampling; Services; Technology; United States National Institutes of Health; Universities; base; improved; instrumentation; mass spectrometer; medical specialties; metabolomics; operation

DESCRIPTION (provided by applicant): The 5600 TripleTOF is a new line of mass spectrometer from AB Sciex that is not yet available to our knowledge in any laboratory in the greater Washington DC area. This proposal requests funds for the purchase of a 5600 TripleTOF mass spectrometer (AB Sciex) based on documented need and thorough evaluation. The 5600 TripleTOF will support funded biomedical applications of mass spectrometry (NIH, DOD, NSF etc) at Georgetown University Medical Center, Washington DC including Lombardi Cancer Center, an NCI designated Comprehensive Cancer Center. Georgetown University has generously supported proteomic research, and promises long term commitment to the maintenance and operation of the mass spectrometric resources. The support from NCRR will be invaluable in helping us upgrade the instrumentation in the current economic climate, and will give us the opportunity to complete projects that cannot be efficiently carried out using the currently available instrumentation at Georgetown University. The mass spectrometric services are centralized in the Proteomic and Metabolomic Shared Resource (PMSR) which has the mandate to make the available technology available to a broad research community at the Georgetown University Medical Center. Proteomic services, directed by Dr. Goldman, cover three major areas of research: 2D-LC-MS/MS characterization of proteomes; quantitative proteomic comparisons; and characterization of protein modifications with specialty services in the analysis of protein glycosylation. The performance of the 5600 greatly improves all these areas of research and perhaps most dramatically the quantitative comparisons of complex samples. Analysis of protein glycoforms is our specialty service with rapidly growing user demand; the 5600 TripleTOF will help us significantly in the development of services in this, in general, insufficiently covered area of research. In this application, we describe several already-funded exciting research projects that will be made possible by the upgrade of our instrumentation to the 5600 TripleTOF.

描述（由申请人提供）：5600 TRIPLET是AB Sciex的新质谱仪系列，我们在大华盛顿特区的任何实验室中尚不可用。该提案要求基于有记录的需求和彻底评估购买5600个质谱仪（AB SCIEX）的资金。 5600 Triplet将支持位于华盛顿特区乔治敦大学医学中心的质谱（NIH，DOD，NSF等）的资助的生物医学应用，其中包括NCI指定综合癌症中心的Lombardi Cancer Center。乔治敦大学（Georgetown University）慷慨地支持蛋白质组学研究，并承诺长期承诺质谱资源的维护和运作。 NCRR的支持对于帮助我们在当前的经济环境中升级工具的支持将是无价的，并将为我们提供机会完成无法使用乔治敦大学目前可用的仪器进行有效进行的项目。质谱服务集中在蛋白质组学和代谢组共享资源（PMSR）中，该资源具有使乔治敦大学医学中心的广泛研究社区可用的技术可用的任务。高盛（Goldman）指导的蛋白质组学服务涵盖了三个主要研究领域：2D-LC-MS/MS蛋白质组织的表征；定量蛋白质组学比较；并在蛋白质糖基化分析中使用专业服务的蛋白质修饰表征。 5600的性能极大地改善了所有这些研究领域，也许最显着地对复杂样本的定量比较。蛋白质糖型的分析是我们的专业服务，其用户需求迅速增长； 5600 Triplet将有助于我们在这项服务的发展中极大地帮助我们的服务领域。在此应用程序中，我们描述了几个已经资助的令人兴奋的研究项目，这些项目将通过我们的仪器升级到5600 Triplet的升级。