OHSU Neuroscience Imaging Center

OHSU 神经科学影像中心

• 批准号: 10169583
• 负责人: SUE A AICHER
• 金额: $ 15.33万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2021-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10169583
• 关键词: 3-Dimensional; Address; Basic Science; Biological Sciences; Brain; Cancer Grant Supplements (P30); Communities; Confocal Microscopy; Consultations; Core Facility; Coupled; Cryoelectron Microscopy; Electron Microscope; Electron Microscopy; Equipment; Face; Fees; Funding; Goals; Grant; Health Sciences; Image; Imaging technology; Institutes; Investments; Light; Manuscripts; Methods; Microscope; Microscopy; Mission; Molecular Structure; National Institute of Neurological Disorders and Stroke; Neurosciences; Neurosciences Research; Optics; Oregon; Pathologic; Primates; Public Health; Publications; Publishing; Qualifying; Research; Research Personnel; Resolution; Resources; Scientist; Services; Technical Expertise; Technology; Time; Training; Transmission Electron Microscopy; United States National Institutes of Health; Universities; University Hospitals; Wages; base; cost; design; digital; experience; experimental study; falls; federal policy; fluorescence imaging; imaging approach; imaging study; immunocytochemistry; in vivo; instrument; instrumentation; light microscopy; microscopic imaging; nervous system disorder; particle; ranpirnase; reconstruction; synergism; technology development; tomography; two photon microscopy

Project Summary The Oregon Health & Science University (OHSU) Neuroscience Imaging Center will provide expertise in designing, conducting, and analyzing experiments on state-of-the-art instrumentation for light and electron microscopy. The Neuroscience Imaging Center has two scientific Cores: Core 1 - Analytical Fluorescence Imaging and Core 2 - Ultrastructure and Single Particle Microscopy. The instruments for these Cores are in place and the technical capacities have been expanded tremendously over the past six years to include not only confocal microscopy and transmission electron microscopy, but also in vivo two-photon microscopy, super-resolution microscopy, EM tomography and single particle analyses for determining molecular structures. All instruments are located in university-wide service centers that are supported by the office of the Vice President for Research. The Neuroscience Imaging Center serves NINDS-funded OHSU researchers, as well as other NIH-funded investigators engaged in neuroscience research. We currently have 21 NINDS- funded investigators who have submitted qualifying projects for the current proposal and have microscopy and imaging needs for their current research. Other OHSU neuroscientists also receive support from Core staff. User fees on shared instruments are partially supported by the P30 grant, with greater support for NINDS than non-NINDS investigators; user fee support is regularly reviewed by the Steering Committee. Microscopy core facilities are located on the Marquam Hill campus and at the adjacent Waterfront Campus (see Resources), and all P30-supported investigators will have open access to all of these resources. The OHSU Neuroscience Imaging Center grant provides salary support for Core staff, who are local, service-oriented technology experts. The Neuroscience Imaging Center Director Dr. Sue Aicher and Associate Director Dr. Catherine Morgans have extensive scientific and technical expertise and will provide front-end consultation with investigators, helping to direct each investigator to the appropriate resources to address their research goals. The Steering Committee provides input to the Center Director with regard to Core strategic and operational issues, as well as project prioritization and Core access plans. We provide different levels of support based on the needs of the investigators. Investigators with extensive imaging expertise primarily require access to state- of-the-art equipment and occasional consultation with staff. Other investigators need more in-depth support, and full service support is often provided for electron microscopy studies. The P30 staff will provide expert support at the appropriate level to meet the needs of our participating investigators. The Neuroscience Imaging Center has catalyzed interactions among NINDS researchers as well as other neuroscientist at OHSU, provided synergy for investments in advanced microscopy, and significantly enhanced scientific progress for our neuroscience community.

项目概要 俄勒冈健康与科学大学 (OHSU) 神经科学成像中心将提供以下方面的专业知识 设计、进行和分析最先进的光和电子仪器的实验 显微镜。神经科学成像中心有两个科学核心： 核心 1 - 分析荧光 成像和核心 2 - 超微结构和单粒子显微镜。这些核心的仪器位于 过去六年来，地点和技术能力得到了极大扩展，包括： 不仅有共焦显微镜和透射电子显微镜，还有活体双光子显微镜， 超分辨率显微镜、电磁断层扫描和单粒子分析用于确定分子 结构。所有仪器均位于由大学办公室支持的全校服务中心 研究副总裁。神经科学成像中心为 NINDS 资助的 OHSU 研究人员提供服务， 以及其他 NIH 资助的从事神经科学研究的研究人员。我们目前有 21 个 NINDS- 资助已提交当前提案合格项目并拥有显微镜和 他们当前研究的成像需求。其他 OHSU 神经科学家也得到核心工作人员的支持。 共享工具的用户费用部分由 P30 赠款支持，对 NINDS 的支持比 非 NINDS 调查员；用户费用支持由指导委员会定期审查。显微镜核心 设施位于 Marquam Hill 校区和邻近的 Waterfront 校区（请参阅参考资料）， 所有 P30 支持的调查人员都可以公开访问所有这些资源。 OHSU 神经科学 影像中心拨款为核心员工提供薪资支持，他们是本地的、以服务为导向的技术人员 专家。神经科学影像中心主任 Sue Aicher 博士和副主任 Catherine 博士 摩根拥有丰富的科学和技术专业知识，并将提供前端咨询 调查员，帮助指导每个调查员获得适当的资源来实现他们的研究目标。 指导委员会就核心战略和运营向中心主任提供意见 问题，以及项目优先级和核心访问计划。我们根据不同的情况提供不同级别的支持 调查人员的需要。具有丰富成像专业知识的研究人员主要需要获得国家 最先进的设备和偶尔与工作人员协商。其他研究人员需要更深入的支持， 通常为电子显微镜研究提供全面的服务支持。 P30工作人员将提供专家 适当级别的支持，以满足我们参与研究人员的需求。神经科学 成像中心促进了 NINDS 研究人员以及其他神经科学家之间的互动 OHSU 为先进显微镜的投资提供了协同作用，并显着增强了科学 我们的神经科学界取得了进步。

项目成果

Prenatal cerebral ischemia disrupts MRI-defined cortical microstructure through disturbances in neuronal arborization.

• DOI: 10.1126/scitranslmed.3004669
• 发表时间: 2013-01-16
• 期刊: Science translational medicine
• 影响因子: 17.1
• 作者: Dean JM;McClendon E;Hansen K;Azimi-Zonooz A;Chen K;Riddle A;Gong X;Sharifnia E;Hagen M;Ahmad T;Leigland LA;Hohimer AR;Kroenke CD;Back SA
• 通讯作者: Back SA

Capsaicin-responsive corneal afferents do not contain TRPV1 at their central terminals in trigeminal nucleus caudalis in rats.

• DOI: 10.1016/j.jchemneu.2014.06.006
• 发表时间: 2014-11
• 期刊: Journal of chemical neuroanatomy
• 影响因子: 2.8
• 作者: Hegarty DM;Hermes SM;Largent-Milnes TM;Aicher SA
• 通讯作者: Aicher SA

Role of Circulating Immune Cells in Stroke and Preconditioning-Induced Protection.

• DOI: 10.1007/978-3-319-18497-5_7
• 发表时间: 2016
• 期刊: Acta neurochirurgica. Supplement
• 作者: Gesuete R;Stevens SL;Stenzel-Poore MP
• 通讯作者: Stenzel-Poore MP

Orientation selectivity in rabbit retinal ganglion cells is mediated by presynaptic inhibition.

• DOI: 10.1523/jneurosci.2081-10.2010
• 发表时间: 2010-11-17
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Venkataramani S;Taylor WR
• 通讯作者: Taylor WR

The effect of long-term ovariectomy on midbrain stress systems in free ranging macaques.

• DOI: 10.1016/j.brainres.2012.09.035
• 发表时间: 2012-12-07
• 期刊: Brain research
• 影响因子: 2.9
• 作者: Bethea CL;Reddy AP
• 通讯作者: Reddy AP