The Analysis of Gene Expression of Morphogenetic Factors Implicated Development of Tooth Germ and Odontogenic Tumors

牙胚发育及牙源性肿瘤形态发生因子基因表达分析

基本信息

批准号：
09470391
负责人：
NAGAI Noriyuki
金额：
$ 6.78万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Tooth morphogenesis and cytodifferentiations are regulated by epithelium-mesenchymal interactions, and the basement membrane plays a role in mediating these interactions Type IV collagen, the major component of the dental basement membrane, is composed of three αchains. Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of α1 to α6 chains of type IV collagen during mouse molar development. We propose that the inner enamel epithlium in the cap stage is composed of type IV collagen consist of α1, α2 and α4 chains, probably in molecular forms of α1(IV) α2(IV) α4(IV), [α4(IV)]ィイD23ィエD2 and [α1(IV)]ィイD22ィエD2 α2(IV). These forms containing α4 chain have not been detected in other organs yet. Therefore, it is thought that these forms is related to morphogenesis and cytodifferentiation characteristic of tooth development that regulated y epithelial-mesenchymal interactions.Recently, it has gradually been ascertained that some growth factors secreted by epithelial or mesenchymal tissue bind to type IV collagen with greater affinity, condens there and contribute to organ morphogenesis in cooperation with basement membrane. We observed the expression patterns of several growth factors (TGF-β, BMPs and FGFs) in the basement menbrane of mouse first molar during tooth germ development and explored the relation between these growth factors and the tooth germ-specific molecular forms of type IV collage. No examined growth factor was detected in the basement membrane in and after cap stage. On the other hand, in the dental lamina and bud stages FGF-2 and TGF-β1 were detected in the dental basement membrane. FGF-2 and TGF-β1 may play an important role in early tooth morphogenesis and cytodifferentiation in cooperation with the molecular form, α3(IV)α4(IV)α5(IV), which exsists in the early stage of tooth development.

牙齿的形态发生和细胞差异受到上皮 - 间充质相互作用的调节，地下膜在介导这些IV型胶原蛋白的相互作用（牙科基底膜的主要成分）中起作用，由三个αChains组成。使用间接免疫荧光在冷冻切除术上，我们表征了小鼠摩尔发育过程中α1蛋白定位到IV型胶原蛋白链的蛋白质定位的变化。我们提出，盖阶段的内搪瓷上皮由IV型胶原蛋白组成，由α1，α2和α4链组成，可能是分子形式的α1（iv）α2（iv）α2（iv）α4（iv）的分子形式，[α4（iv）] II D23E D2 D2 D2 D2和[α1（II II II d2）这些包含α4链的形式尚未在其他器官中检测到。因此，人们认为这些形式与牙齿发育的形态发生和细胞区分学特征有关，该特征调节了上皮 - 间质相互作用。实际上，已经逐渐确定某些由上皮或间质组织与IV型与IV类型胶原蛋白结合的某些生长因子与在其中结合，并在其中缩短了培养物，并在其贡献中构成了培训，并在其上结合了培训。我们观察到在牙齿发育过程中，小鼠第一磨牙的基底末端的几种生长因子（TGF-β，BMP和FGFS）的表达模式，并探索了这些生长因子与IV型类分子的分子形式之间的关系。在盖阶段和盖子后，在基底膜中未检测到生长因子。另一方面，在牙科膜上检测到牙齿层和芽阶段FGF-2和TGF-β1。 FGF-2和TGF-β1可能在与分子形式合作的早期牙齿形态发生和细胞差异中起重要作用，α3（iv）α4（iv）α5（iv）α5（iv）在牙齿发育的早期阶段就存在。