Expression of Cytokeratins in Odontogenic Tumors.

细胞角蛋白在牙源性肿瘤中的表达。

基本信息

批准号：
03807120
负责人：
NAGAI Noriyuki
金额：
$ 1.15万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Cytokeratins, intermediate filaments characteristic of epithelial cells, are well known to be closely related to epithelial differentiation. That is, the differentiation specificity to expression of different composition of cytokeratins has been described in epithelial cell types. Along these approaches, the expression of cytokeratins has been observed in the odontogenic epithelium and analyzed with special reference to the relationship between tooth germs and odontogenic cysts and tumors. But the distribution of cytokeratins has been unlikely to explain satisfactorily the origin of odontogenic tumors, because of heterogenous expression of cytokeratins in odontogenic tumors.1.Ameloblastoma ; Interestingly, they presented difference of cytokeratin distribution between follicular and plexiform types. In follicular ameloblastomas, the central portion showed immunoreactivity with all markers of cytokeratins, SE-K, NSE-K and K19. But the columnar cells arranged in the periphery were reacted … More positively for K19, faintly reacted for NSE-K, and negatively responded for the marker of squamous cells, SE-K. In plexiform ameloblastoma both central and peripheral cells demonstrated the positive reaction with all markers, SE-K, NSE-K and K19.2.Adenomatoid Odontogenic Tumors ; Adenomatoid odontogenic tumors demonstrated different distribution between central areas composed of columnar cells and peripheral areas of small round cells. Most areas of central cells, showing whorled structure or solid nests, demonstrated no expression for the marker of squamous cells, SE-K, however, only a few areas showed faint positivity. The immunoreactivity for NSE-K and K19 was so heterogenous that both immunoreactive and non-reactive areas were present within the central area. Ductal or tubular structures also showed no immunoreactivity for the marker of squamous cells (SE-K) with positive reaction for NSE-K and K19. The cells surrounding central whorled structures were smaller than central columnar cells and were proliferated without organizing pattern. These peripheral cells showed positive reaction for all markers of cytokeratins. Areas of reticular pattern composed of small round cells, which was frequently found in adenomatoid odontogenic tumors, also showed the same cytokeratin distribution with peripheral cells.3.Enamel Organ ; Along the maturation to enamel organ from dental lamina, the immunoreaction for the marker of squamous differentiation, SE-K, was negatively reacted. NSE-K and K19 were well demonstrated in the enamel organ proper. Less

细胞角蛋白是上皮细胞表征的中间细丝，众所周知与上皮分化密切相关。也就是说，在上皮细胞类型中已经描述了对不同组成蛋白表达的分化特异性。沿着这些方法，在牙源性上皮中观察到了细胞角蛋白的表达，并特别参考了牙齿细菌与牙源性囊肿和肿瘤之间的关系。但是，由于细胞源性肿瘤的异质表达在牙源性肿瘤中，细胞角蛋白的分布不太可能令人满意地解释牙源性肿瘤的起源。有趣的是，他们提出了细胞角蛋白分布在卵泡和丛状类型之间的差异。在卵泡膜细胞瘤中，中央部分显示了细胞角蛋白，SE-K，NSE-K和K19的所有标记物的免疫反应性。但是排列在外围的柱状细胞反应……对K19更积极地对NSE-K反应，并对鳞状细胞的标记se-k做出负面反应。在丛状木质母细胞瘤中，中央细胞和外周细胞都表现出与所有标记物SE-K，NSE-K和K19.2.Adenomatioid牙源性肿瘤的阳性反应。腺样牙源性肿瘤在由柱状细胞和小圆形细胞的外围区域组成的中心区域之间显示出不同的分布。中央细胞的大多数区域，显示所有结构或固体巢，对于正方形细胞标记的SE-K标记没有表达，但是，只有少数几个区域准确地显示出来。 NSE-K和K19的免疫反应性是如此异质，以至于中央区域内都存在免疫反应性和非反应性区域。导管或管状结构也没有针对NSE-K和K19阳性的方形细胞（SE-K）标记物的免疫反应性。围绕中心的细胞，任何小于中央柱状细胞都小的结构，并在没有组织模式的情况下被增殖。这些周围细胞对所有细胞角蛋白标记的呈阳性反应。由小圆形细胞组成的网状模式的区域，经常在腺样牙基因源性肿瘤中发现，也显示出具有外围细胞的相同细胞角蛋白分布。3.Enamel器官。沿着牙齿层的成熟到牙釉质器官，对鳞状分化标记的免疫反应SE-K反应。 NSE-K和K19在适当的搪瓷器官中得到了很好的证明。较少的