Development of high Tc SQUID microscope for biological application

生物应用高Tc SQUID显微镜的开发

基本信息

批准号：
09555128
负责人：
ENPUKU Keiji
金额：
$ 8.32万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09555128/
关键词：
SQUID magnetometer SQUID microscope magnetic nanoparticles immunoassays magnetic marker high Tc superconductor 1 f noise 30-degree bicrystal junction SQUID 磁気センサジョセフソン素子バイクリスタル基板ノイズ磁束ノイズ

项目摘要

In this research, we developed high Tc SQUID magnetic-microscope system for biological application. Our results are summarized as follows.1. We developed high performance SQUID utilizing bicrystal junctions with 30-degree misorientation angles. At 77 K, we can routinely obtain the voltage modulation depth ΔV>20μV and the flux noise SィイD2φィエD2ィイD11/2ィエD1<12μφィイD2oィエD2/HzィイD11/2ィエD1 for 100pH-SQUID.2. We significantly reduced the 1/f noise of the direct-coupled magnetometer. In order to avoid the flux trapping in the pickup coil, we develop a pickup coil which consists of 4 parallel loop with narrow line-width and involves the so-called flux dam. With this pickup coil, the flux noise is reduced to SィイD2φィエD2ィイD11/2ィエD1=23μφィイD2oィエD2/HzィイD11/2ィエD1 at f=1 Hz. This values is only 2.3 times higher than the white noise of SィイD2φィエD2ィイD11/2ィエD1=10μφo/HzィイD11/2ィエD1.3. We developed a SQUID microscope system in order to detect FeィイD22ィエD2OィイD23ィエD2 magnetic nanoparticles. A special Dewar to make a distance between the SQUID and sample as small as 1 mm is developed. The system works well even when the magnetic field of 10 Gauss is applied in parallel to the SQUID.4. Biological immunoassay is performed with the present system. In the present system, as antibody is labeled with FeィイD22ィエD2OィイD23ィエD2 nanoparticles. Binding reaction between antigen and its antibody can be detected by measuring the magnetic field from FeィイD22ィエD2OィイD23ィエD2 nanoparticles. It is shown that the present system is 10 times more sensitive than the conventional method using an optical marker. It will be possible to improve the sensitivity by more than factor 10.

在这项研究中，我们开发了用于生物学应用的高TC鱿鱼磁性微镜系统。我们的结果总结如下1。我们利用具有30度不良角度的生物交界处开发了高性能鱿鱼。在77 K时，我们可以逐渐获得电压调制深度ΔV>20μV，而通量噪声squidd2φd2iid11/2i d1 <12μd22OD2/Hz D11/2i D1对于100ph-squid.2。我们显着降低了直接耦合磁力计的1/F噪声。为了避免拾取线圈中的通量捕获，我们开发了一个拾音器线圈，该拾音器由4个平行环组成，并涉及所谓的通量大坝。使用此拾音器线圈，将通量噪声还原为SiliD2φD2ID11/2I D1 =23μφD2OD2/Hz D1/211/2i D1在F = 1 Hz时。该值仅比鱿鱼D2φD2IID11/2II D1 =10μφO/Hz D11/2II D1.3的白噪声高2.3倍。我们开发了一个鱿鱼显微镜系统，以检测FEI D22II D2OII D23II D2磁性纳米颗粒。开发了一种特殊的露水，以在小至1毫米的情况下建立距离。即使在与鱿鱼并行应用10高斯的磁场时，系统也可以很好地工作。4。生物免疫测定是通过当前系统进行的。在当前系统中，由于抗体用FEI D22，D2OI D23，D2纳米颗粒标记。可以通过测量FEI D22，D2OI D23，D2纳米颗粒的磁场来检测抗原与其抗体之间的结合反应。结果表明，使用光学标记物比常规方法高10倍。可以将灵敏度提高超过因子10。