Magnetic immunoassay system utilizing magnetic marker and SQUID sensor

利用磁标记和 SQUID 传感器的磁免疫分析系统

• 批准号: 15360220
• 负责人: ENPUKU Keiji
• 金额: $ 9.41万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15360220/
• 关键词: SQUID sensor; High Tc superconductor; Magnetic nanoparticle; Magnetic relaxation; magnetic marker; Immunoassay; antigen-antibody reaction; 吸着

Immunoassays are widely used to detect disease-related proteins for medical diagnostics. These proteins are generically called antigens, and the so-called binding reaction between antigen and its antibody is used for the immunoassay. In order to improve the performance of the immunoassay, we have developed a new detection system utilizing a magnetic marker and a SQUID sensor. The main results obtained in this study are as follows.(1)We clarified the dependence of the magnetic properties on the size of the magnetic nanoparticle. We showed that the particle with nominal diameter of 25 nm can keep remanence, and can generate large magnetic signal. This particle is, therefore, suitable for magnetic immunoassay.(2)We developed a coating process to cover the magnetic particle with polymer. With this process, we fabricated a polymer-coated magnetic nanoparticle that shows good dispersion in liquid.(3)We developed the high Tc SQUID system for detecting magnetic signal from the marker. Since the signal decays rapidly in space, we realized a close distance between the cooled SQUID and the room temperature sample.(4)We developed a magnetic-contamination free reaction chamber and a method to cancel environmental noise in order to detect very weak signal. Our system can detect 12 samples in a short measurement time of a few minutes with a field resolution of about 10 pT.(5)We conducted a detection of protein called IgE. We demonstrated the detection of IgE down to 0.3 pg, which shows the high sensitivity of the present magnetic method. We also demonstrated the detection of IgE in human serum, which shows the possibility to practical diagnosis.

免疫测定法被广泛用于检测与疾病相关的蛋白质进行医学诊断。这些蛋白通常称为抗原，抗原与其抗体之间的所谓结合反应用于免疫测定。为了提高免疫测定的性能，我们使用磁标记和鱿鱼传感器开发了一个新的检测系统。在这项研究中获得的主要结果如下。（1）我们阐明了磁性特性对磁性纳米颗粒尺寸的依赖性。我们表明，名义直径为25 nm的粒子可以保持延迟，并且可以产生较大的磁信号。因此，该粒子适用于磁性免疫测定。（2）我们开发了一个涂层过程，用聚合物覆盖磁性颗粒。在此过程中，我们制造了一个聚合物涂层的磁性纳米颗粒，该磁性纳米颗粒在液体中显示出良好的分散。（3）我们开发了高TC Squid系统，用于从标记中检测磁信号。由于信号在太空中迅速衰减，因此我们意识到冷却的鱿鱼和室温样品之间的距离很近。（4）我们开发了一个磁性污染的无反应室，以及一种取消环境噪声的方法，以检测非常弱的信号。我们的系统可以在几分钟的短时间测量时间中检测到12个样品，而场分辨率约为10分。（5）我们进行了一种称为IgE的蛋白质检测。我们证明了将IGE降低到0.3 pg的检测，这表明了当前磁方法的高灵敏度。我们还证明了人血清中IgE的检测，这表明了实际诊断的可能性。

项目成果

T.Q.Yang et al.: "SQUED magnetometer utilizing normal pickup coil and resonant-type coupling circuit"Physica C. 392-396. 1396-1400 (2003)

T.Q.Yang 等人：“利用普通拾波线圈和谐振型耦合电路的 SQUED 磁力计”Physica C. 392-396。

Design of superconducting quantum interference device for magnetic immunoassays

磁免疫分析超导量子干涉装置的设计

• 发表时间: 2004
• 期刊: Jpn.J.Appl, Phys. Vol.43 No.9A
• 作者: 栗原;清水;小野;安藤;K.Enpuku et al.;K.Enpuku;K.Enpuku et al.;T.Q.Yang et al.;K.Enpuku et al.;T.Q.Yang et al.;T.Q.Yang et al.;K.Enpuku et al.
• 通讯作者: K.Enpuku et al.

Detection of magnetic nanoparticles with ac susceptibility measurement

• DOI: 10.1016/j.physc.2004.01.146
• 发表时间: 2004-10-01
• 期刊: PHYSICA C-SUPERCONDUCTIVITY AND ITS APPLICATIONS
• 影响因子: 1.7
• 作者: Yang, TQ;Abe, M;Enpuku, K
• 通讯作者: Enpuku, K

高温超伝導SQUIDを用いた免疫検査システムの開発

使用高温超导SQUID的免疫分析系统的开发

• 发表时间: 2004
• 期刊: 日本応用磁気学会第137回研究会 資料
• 作者: N.Hozumi;R.Nagae;C-K.Lee;M.Nagao;K.Kobayashi;Y.Saijo;M.Tanaka;et al.;T.Q.Yang et al.;K.Enpuku et al.;円福敬二;円福敬二
• 通讯作者: 円福敬二

K.Enpuku et al.: "High Tc SQUID system and magnetic marker for biological immunoassay"IEEE Trans.Appl.Supercond.. Vol.13,No.2. 371-376 (2003)

K.Enpuku等人：“用于生物免疫测定的高Tc SQUID系统和磁性标记”IEEE Trans.Appl.Supercond..Vol.13，No.2。