Molecular and Cellular Analyses of Self and Non-self Recognition Systems in Plants

植物自我和非自我识别系统的分子和细胞分析

• 批准号: 09480195
• 负责人: ISOGAI Akira
• 金额: $ 7.04万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480195/
• 关键词: self-incompatibility; S-gene; pollen S-determinant; Brassica; phytopathogenic bacteria; fragellin; Pseudomonas avenae; self and non-self recognition; S遺伝子; 受容体型キナーゼ; レセプターキナーゼ; 植物病原細菌

(1)Analysis of pollen S-determinant in self-recognition system of Brassica.In the self-incompatibility system of Brassica, two stigma S-determinants have been known ; S-receptor kinase (SRK) and S-locus glycoprotein (SLG). But the pollen S-determinant was still uncertain. We have analyzed pollen extract and pollen mRNA of Brassica campestris using various biochemical and molecular biological methods and succeeded in founding candidate molecules of the pollen S-determinant. Then we analyzed it S-specificity, linkage to S-locus and expression pattern in anther and pollen. Further we performed an in vitro assay and transformation experiment for the pollen S-determinant, and we confirmed that the gene tentatively termed SP11 is the pollen S-determinant.(2)Recognition molecules in host and non-host relation of plant-pathogen interaction.Pseudomonas avenae is a phytopathogenic bacterium that attacks widely monocot plants. But the host-specificity in the level of strains is rather strict ; some strains can attack rice but not other plant species. When an incompatible strain would infect rice, it induces hypersensitive reactions and caused cell death. As a molecule to be recognized as a marker of an incompatible strain, a fragellin was identified by a biochemical method. We further proved that a fragellin-less mutant strain cannot cause hypersensitive reactions.

(1)甘蓝类自交不亲和系统中花粉S决定子的分析。在甘蓝类自交不亲和系统中，已知有两个柱头S决定子； S-受体激酶 (SRK) 和 S-位点糖蛋白 (SLG)。但花粉 S 决定因素仍不确定。我们利用多种生化和分子生物学方法对白菜花粉提取物和花粉mRNA进行了分析，并成功建立了花粉S决定子的候选分子。然后我们分析了它的S-特异性、与S-位点的连锁以及在花药和花粉中的表达模式。进一步对花粉S决定子进行了体外测定和转化实验，证实暂定名为SP11的基因就是花粉S决定子。(2)植物与病原菌相互作用的寄主和非寄主关系中的识别分子燕麦假单胞菌是一种广泛侵袭单子叶植物的植物病原细菌。但菌株水平上的宿主特异性相当严格；有些菌株可以攻击水稻，但不能攻击其他植物物种。当不相容的菌株感染水稻时，它会引起过敏反应并导致细胞死亡。作为被识别为不相容菌株标记的分子，通过生化方法鉴定了脆杆蛋白。我们进一步证明，不含脆杆蛋白的突变株不会引起过敏反应。

项目成果

G.Suzuki: "Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector." Gene. 199. 133-137 (1997)

G.Suzuki：“使用 P1 衍生的人工染色体 (PAC) 载体直接克隆芸苔属 S 基因座。”

G.Suzuki: "Genomic oraganization of the S locus : Identification and charasterization of genes in SLG/SRK ragion of S9 faplotype of Brassica campestris (svn.rap).,"Genetics. 153. 391-400 (1999)

G.Suzuki：“S 基因座的基因组组织：白菜 S9 倍型 (svn.rap) 的 SLG/SRK ragion 中基因的鉴定和表征。”遗传学。

T. Takasaki: "Introduction of SLG (S locus glycoprotein) alters the phenotype of endogenous S haplotype, but confers no new S haplotype specificity in Brassica rapa L"Plant Mol. Biol.. 40. 659-668 (1999)

T. Takasaki：“SLG（S 位点糖蛋白）的引入改变了内源 S 单倍型的表型，但没有赋予白菜新的 S 单倍型特异性”Plant Mol。

G.Suzuki: "Genomic structure of SLG9 nad SRK9 genes in self-incompatible Brassica campestris L." J.Reprod.Develop.43(suppl). 165-166 (1997)

G.Suzuki：“自交不亲和甘蓝中 SLG9 和 SRK9 基因的基因组结构。”

高山誠司: "アブラナ科植物の自家不和合性の分子機構" 蛋白質・核酸・酵素. 42(9). 1386-1395 (1997)

Seiji Takayama：“十字花科植物自交不亲和的分子机制”蛋白质、核酸和酶 42(9) 1386-1395 (1997)。