BIO-ORANIC CHEMICAL REEARCH ON CELL SIGNALS IN BACTERIA

细菌细胞信号的生物有机化学研究

基本信息

批准号：
12460053
负责人：
ISOGAI Akira
金额：
$ 9.15万
依托单位：
NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

1. Fruiting body formation in Myxococcus, Stigmatellaaurantiaca.Stingmolone is a fruiting body inducing factor secreted from the cells of Stigmatella aurantiaca when the cells are starved and irradiated with light. Two genes, RelA and csgA are thought to be involved in fruiting body formation in another Mkococcus in which any low molecular weight factor like stigmolone has not been known. In this study, we isolated homologs to these genes from S. aurantiaca and made the bacterial mutants using these genes. In RelA disrupted mutant, the productivity of stigmolone was lost. The ability to form fruiting body could not be recovered by addition of stigmobne. So, RelA should be involved in requirement of receptability as well as productivity of stigmolone. The gene, csgA promoted the synthesis of stigmolone The Mrna of the gene was induced by starvation but not by light irradiation.2. Plant-pathogen interaction of phytopathogenic bacteria.(1) Phytotoxin produced by Pseudomonas syringae pv. syringae.The gene cluster for synthesis of Phytotoxin in P. syringe pv. syringae was isolated and the gene was disrupted. In the cultured medium of the mutant bacterium, the toxin was not detected. When the mutant was applied to a susceptive plant, the growth of the bacterium was inhibited and the disease symptom was much diminished. Then the toxin should be involved in the pathogenecity of the bacterium.(2) Flagellins of Pseudomonas avenaeFlagellin of P. avenae is involved in induction of the hypersensitive reaction (HR) to rice in strain species specific manner. The bacterial genes from pathogenic and non-pathogenic strains were expressed in E. coli. Both of the expressed proteins could similarly elicit HR to rice cultured cells. So it is concluded that the specificity of the flagelns does not depend on the difference of the amino acid sequences.

1。在粘膜球菌中的果实形成，Stigmatellaaurantiaca。Stingmolone是一种结实的身体，当细胞饥饿并用光照射时，诱导了从stigmatella aurantiaca细胞分泌的诱导体。据认为，RELA和CSGA的两个基因参与了另一个Mkococcus中的果实形成，其中尚不清楚任何低分子重量因子（如Stigmolone）。在这项研究中，我们将这些基因分离为aurantiaca，并使用这些基因使细菌突变体。在Rela中断的突变体中，Stigmolone的生产力丢失了。形成水果体的能力无法通过增加污名来恢复。因此，RELA应参与柱头的接受性和生产力的要求。 CSGA基因促进了stigmolone的合成。基因的mRNA是由饥饿诱导的，但不是通过光照射诱导的。2。植物病毒细菌的植物 - 病原体相互作用。（1）假单胞菌丁香PV产生的植物毒素。 Syringae。用于合成植物毒素的基因簇。丁香科被隔离，并破坏了基因。在突变细菌的培养培养基中，未检测到毒素。当将突变体应用于敏感植物时，细菌的生长被抑制，疾病症状大大减少。然后，毒素应参与细菌的病原体。（2）假单胞菌假单胞菌的鞭毛蛋白鞭毛蛋白酶与特定于菌株的方式诱导了对水稻的超敏反应（HR）的诱导。在大肠杆菌中表达了来自致病性和非致病性菌株的细菌基因。两种表达的蛋白质都可以类似地引起水稻培养细胞的HR。因此得出的结论是，鞭毛的特异性不取决于氨基酸序列的差异。