Development of the gene therapy against the organ dysfunction in the patients under LVAS support.

针对 LVAS 支持下患者器官功能障碍的基因疗法的开发。

基本信息

批准号：
09557109
负责人：
SAWA Yoshiki
金额：
$ 8.64万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557109/
关键词：
LVAS liver dysfunction microcirculation LVAS 術後肝障害

项目摘要

1. To forecast the hyper bilirubinemia in the patients after operation, we evaluated the preoperative factors (hepatocyte damage, hepatic sinusoid-endothelial dysfunction, liver function and inflammatory response). Operations performed were mitral valve replacement. We measured the serum levels of aspartate transaminase (AST), alanine transaminase (ALT), hyaluronic acid (HA), total bilirubin (TB), choline estelase (ChE), interleukm-6 (IL-6), interleukin-8 (IL-8) and CRP before operation. We evaluated the correlation between these indexes and the peak level of TB in seven post-operative days. The levels of preoperative TB, ChE, I-IA and IL-6 had a good correlation with the peak level of postoperative TB.These results suggested that liver function, hepatic sinusoid-endothelial dysfunction and inflammatory reaction may play a significant role in forecast of postoperative hepatic failure in patients.2. To clarify the mechanism of hyper-bilirubinemia in the setting of left ventricular assis … More t device (LVAS), we evaluated the change of hepatocellular function, hepatic sinusoid-endothelial microcirculation and inflammatory response before and after LVAS implantation. We measured serum levels of total bilirubin (TB), transaminases (ALT,AST), interleukin (IL-6, IL-8), and HA ; a indicater for hepatic sinusoidal circulation, before and after LVAS implantation. The level of I-LA and IL-8, had a good correlation with the level of TB.However, the AST, ALT or 11-6 did not relate to the alternation of TB.These results suggested that hepatic sinusoid-endothelial dysfunction and inflammatory reaction may play a significant role in hepatic failure in patients following implantation of LVAS.3. Under the current situations we have increasing opportunities to manage the patients with posthepatitis and/or congestive liver dysfunction. In order to prevent postoperative hepatic failure we described perioperative management for those patients. We have no effective methods to estimate the functional reserve, but our data suggested that serum cholineesterase level at the preoperative states might demonstrate prognostic significance. Less

1。为了预测手术后患者中胆红素血症的高脂蛋白血症，我们评估了术前因子（肝细胞损伤，肝张正弦性内皮功能障碍，肝功能和炎症反应）。执行的操作是二尖瓣更换。我们测量了天冬氨酸转氨酸酶（AST），丙氨酸转氨酶（ALT），透明质酸（HA），总胆红素（TB），胆碱酯酶（CHE），Interleukm-6（IL-6），IL-6），Iltleukin-8（ILL-8）（IL-8）和CRP的血清水平。我们评估了这些指数和在七个操作后的TB的峰值水平之间的相关性。术前结核病，CHE，I-IA和IL-6的水平与操作后结核的峰值水平有很好的相关性。这些结果表明，肝功能，肝正正弦内皮功能障碍和炎症反应可能在患者的术后肝衰竭预测中起重要作用。2。为了阐明在左心室囊泡的环境中高脂蛋白血症的机制……更多的T装置（LVA），我们评估了LVAS植入前后的肝细胞质功能，肝曲张正弦粘体性微循环和炎症反应的变化。我们测量了总胆红素（TB），转氨酶（ALT，AST），白介素（IL-6，IL-8）和HA的血清水平； LVA植入之前和之后的肝曲正弦循环指标。 I-LA和IL-8的水平与TB的水平有很好的相关性。但是，AST，ALT或11-6与TB的替代方案无关。这些结果表明，丙型肝张正弦内皮功能障碍和炎症反应在肝瘤患者中可能在LVAS.3遵循肝的患者中起重要作用。在当前情况下，我们有更多的机会管理雌科后和/或充血性肝功能障碍的患者。为了防止术后肝炎衰竭，我们描述了这些患者的定期治疗。我们没有有效的方法来估计功能储备，但是我们的数据表明，术前状态的血清胆固醇化酶水平可能表现出预后的意义。较少的