Development of Myocardial Regenerative Therapy using Highly Regular Honeycomb-Patterned Scaffold inducing tissue self-regeneration

使用高度规则的蜂窝图案支架诱导组织自我再生开发心肌再生疗法

• 批准号: 16209042
• 负责人: SAWA Yoshiki
• 金额: $ 25.79万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209042/
• 关键词: honeycomb-patterned film; scaffold; Recellularization; Myocardial Regenerative; ナノハニカムシート; 心筋再生治療; 生分解性多孔性足場; 生分解性多孔体足場; 心筋再生治療法

Cell therapy for myocardial repair after myocardial infarction is a new and promising strategy. However, cell therapy generally needs to culture the cell for a long period in order to increase a sufficient number of cells. To solve this problem, it needs to use a suitable scaffold for cell attachment, proliferation, differentiation and several functions. In this study, we evaluated the effect of surface topography in the cell for myocardial cell therapy behavior using the highly regular patterned biodegradable films by simple casting technique.The bon marrow-derived mesenchymal stem cell and myoblast proliferation was significantly accelerated on the honeycomb-patterned film as compared to the flat films. On the stretched honeycomb-patterned films, the cardiac cell morphology was correctly aligned to the stretch direction and not aligned on the normal films. Recellularization of honeycomb-patterned film was observed in vivo.The highly regular patterned films with controlled pore size and surface topography have a strong effect on the cell proliferation differentiation and alignment. The honeycomb-patterned films may be a novel candidate of the scaffold for Myocardial Regenerative Therapy

心肌梗塞后心肌修复的细胞疗法是一种新的且有前途的策略。但是，细胞疗法通常需要长时间培养细胞，以增加足够数量的细胞。为了解决此问题，它需要使用合适的支架来进行细胞附着，增殖，分化和多种功能。在这项研究中，我们使用简单的铸造技术评估了细胞中表面形貌在细胞中对心肌细胞疗法行为的影响。与平面膜相比，在Honeycomb-Pertanned Films上显着加速了Bon Marrow衍生的中质干细胞和肌细胞增殖。在拉伸的蜂窝图案中，心脏细胞的形态正确地对齐到拉伸方向，而在正常膜上没有对齐。在体内观察到了蜂窝图案的胶片胶片。具有控制的孔径和表面地形的高度规则的图案膜对细胞增殖分化和比对具有很强的影响。蜂窝图案的电影可能是心肌再生疗法脚手架的新颖候选者