Development of reagents for effective genetic modification of plants

开发有效植物基因改造试剂

基本信息

批准号：
09556024
负责人：
KANZAKI Hiroshi
金额：
$ 7.3万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09556024/
关键词：
crown gall Agrobacterium plant transformation inhibitor inhibition of bacterial attachment acidic polysaccharide cellulose biosynthesis cyclic b1, 2-glucan adhesin cyclic β1,2-glucan rhicadhesin 幾何異性体

项目摘要

Tow novel geometrical isomers of oxazolomycin, oxazolomycins B (2) and C(3), were isolated from the fermentation broth of an oxazolomycin- producing strain, Streptomyces albus JA3453. The configurations of the triene moieties of 2 and 3 are (4'E, 6'E, 8'E) and (4'Z, 6'E, 8'E), respectively, while that of oxazolomycin (1) is (4'Z, 6'Z, 8'E). 2 and 3 exhibited potent inhibitory activity against crown gall formation with the same MIC (0.8 μg/disk) as oxazolomycin. 2 and 3 showed no antibacterial activity against Agrobacterium tumefaciens, in contrast to oxazolomycin which has specific anti-A. tumefaciens activity. The newly established GUS expression bioassay of the plant callus extracts revealed that the extracts of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. We found that the inhibitory activity of oxazolomycin oxazolomycin diacetate against plant transformation was due to its inhibitory effect against the attachment of A. tumefaciens to host plants. Furthermore, oxazolomycin diacetate was found to exhibit an inhibitory activity against the rhicadhesin-mediated agrobacterial attachment to plants, not the biosynthesis of cellulose and β-1, 2-glucan which have been reported to be involved in the agrobacterial attachment. And we found that a novel bacterial acidic polysaccharide from a soil bacterium exhibited plant transformation enhanced plant transformation due to its stimulatory effect for the attachment of A. tumefaciens to host plants.

从黄唑菌素B（2）和C（3）的拖曳新型的几何异构体中，是从黄唑霉素菌株的发酵液中分离出来的。 2和3的三烯部分的配置分别为（4'e，6'e，8'e）和（4'z，6'e，8'e），而牛唑霉素（1）的配置为（4'z，6'z，6'z，8'e）。 2和3表现出与黄唑霉素相同的MIC（0.8μg/磁盘）的冠状胆囊形成的潜在抑制活性。 2和3与具有特定抗A的黄唑霉素相比，没有针对农杆菌的抗菌活性。 Tumefaciens活动。植物愈伤组织提取物的新建立的GUS表达生物测定表明，植物菌的提取物的提取物具有针对农业植物转化的最潜在抑制活性。从提取物中分离出三萜糖苷phytolaccoside b，作为具有抗细菌或植物毒性活性的真正植物转化抑制剂。我们发现，阿沙唑霉素二硫代乙酸对植物转化的抑制作用是由于其抑制作用对植曲霉的附着在宿主植物上的抑制作用。此外，发现二硫酸二唑霉素会杀死对植物粘着蛋白介导的生物细菌的抑制活性，而不是纤维素和β-1，2-葡聚糖的生物合成，这些生物合成据报道涉及农杆菌依恋。我们发现，由于其刺激性作用，一种新型细菌酸性多糖暴露于植物变化，从而增强了植物转化，这是由于其刺激性的效果，可将曲霉杆菌附着在宿主植物上。