CHARACTERIZATION OF RAT THERMOSTATS

大鼠恒温器的特性

• 批准号: 09670069
• 负责人: KOBAYASHI Shigeo
• 金额: $ 2.3万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670069/
• 关键词: THERMOSTAT; CLONING; WARM; CHANNEL; mRNA; 発現クローニング; cDNAライブラリー; 後根神経節; 視床下部; 卵母細胞; 冷受容ニューロン; 温度受容ニューロン; F-11

We have identified that warm sensitive cells in the hypothalamus and warm-receptors in the skin act as thermostats for thermoregulation. Patch clamp studies of hypothalamic warm-sensitive neurons suggest that warming-activated cation channel is key factor of the thermostats. The purpose of this study is molecular cloning of genes relevant to the thermostat. We used F-11 (cell line derived from dorsal root ganglion) as a source of mRNA.1. Warming increased intracellular CaィイD12+ィエD1 level [CaィイD12+ィエD1]I in F-11 cells, detected by Ca Fura-2AM.2. Warming induced whole-cell inward current in F-11 cells voltage clamped at -60mV.3. Oocytes infected with F-11 mRNA responded to warming, detected by two-electrode voltage-clamp method. First, warming induced current continuously Second, warming induced current transiently and inactivated soon.We plan to make f-11 cDNA library for molecular cloning of genes relevant to thermostats.

我们已经确定下丘脑中的温暖敏感细胞和皮肤中温暖的受体充当温度调节的恒温器。下丘脑温敏神经元的斑块夹研究表明，变暖激活的阳离子通道是恒温器的关键因素。这项研究的目的是与恒温器相关的基因的分子克隆。我们使用F-11（衍生自背根神经节的细胞系）作为mRNA的来源。加热增加了细胞内CAI D12+E D1水平[CAI D12+E D1]在F-11细胞中，由Ca Fura-2am.2检测到。变暖诱导的F-11细胞电压夹在-60MV.3处的F-11细胞电流。感染了F-11 mRNA的卵母细胞对变暖的反应，通过两电极电压钳法检测到了变暖。首先，变暖诱导的电流连续第二，变暖诱导的电流瞬时并灭活。

项目成果

KOBAYASHI, Shigeo: "Seitai Joho Shori"Korona-sha. 200 (2000)

小林茂夫：《Seitai Joho Shori》Korona-sha。

小林茂夫: "生体情報処理"コロナ社. 200 (2000)

小林茂雄：《生物信息处理》Coronasha 200 (2000)。

Shigeo Kobayashi: "Warming activated channels of warm-sensitive neurons in rat hypothalamic slices"Neuroscience Letters. 273. 1-4 (1999)

Shigeo Kobayashi：“大鼠下丘脑切片中热敏感神经元的变暖激活通道”神经科学快报。

小林茂夫: "生体情報処理-生体サーモスタットから見えてくる生体情報系" コロナ社, 230 (2000)

Shigeo Kobayashi：“生物信息处理 - 从生物恒温器中看到的生物信息系统”Coronasha，230（2000）