Identification of dermal papilla derived agents which transform the terminalhair to vellus hair

将终毛转变为毳毛的真皮乳头衍生剂的鉴定

基本信息

批准号：
09670882
负责人：
ARASE Seiji
金额：
$ 2.3万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670882/
关键词：
male pattern baldness dermal papilla dermal papilla specific genes DNA sequence specific anti-peptide antibody thyroid hormone receptor monoclonal antibody keratin 毛乳頭細胞毛乳頭特異的遺伝子軟毛硬毛ノックアウトマウス

项目摘要

(1). Two types of dermal papilla cells were cultured from the excised hair follicles obtained from the bald lesion and non bald occipital lesion of a male subject afflicted with an androgenic alopecia. Then total RNAs were extracted from those two tupes of dermal papilla cells (DPCs), and 3'-directed cDNA libraly were constructed using oligo-dT tailed pUC19 as a primer. Approximately 1000 each colonies were picked up randomly for both libraries, and nucleotide sequence of each cDNA was determined, and gene expression profiles were compared with the data base (BodyMap) ose. The we categorize them into 3 groups ; 1) new genes highly expressed in normal control DPCs but not in the bald DPCs. 2) new genes highly expressed in the bald DPCs but not in normal control DPCs. 3) known genes whose expression is highly different between the two. Type I collagen, fibronectin, and osteonectin, which were cloned ten times or more in both libraries, which means that DPCs abundantly produce those extracellular matrix. In sillicob comparison of the genes identified control DPC-specific and bald DPC-specific genes, all of which were speciffically expressed in dermal papilla cells. Finally we chose 8 candidate genes and whose expression profiles and functions are now being studied by means of developing knock-out animals of those genes.(2). Thyroid hormone receptor expression was examined in the hair folicle. All cells which compose the hair follicle were T3 receptor positive. In addition, the activity of T3 on the growth of cultured hair follicle cells is highest at the phisiological concentration.(3). Monoclonal antibodies against human dermal papilla were obtained. One of which was confirmed to recognize a new keratin.

(1).从患有雄激素性脱发的男性受试者的秃头病变和非秃头枕骨病变获得的切除毛囊中培养两种类型的真皮乳头细胞。然后从这两种真皮乳头细胞(DPC)中提取总RNA，并使用oligo-dT加尾的pUC19作为引物构建3'导向的cDNA文库。对于两个文库，每个菌落随机挑选约1000个，确定每个cDNA的核苷酸序列，并将基因表达谱与数据库(BodyMap)进行比较。我们将它们分为 3 组； 1）新基因在正常对照DPC中高表达，但在秃头DPC中不表达。 2)新基因在秃头DPC中高表达，但在正常对照DPC中不表达。 3) 两者之间表达高度不同的已知基因。 I 型胶原蛋白、纤连蛋白和骨连接蛋白在两个文库中都被克隆了十次或更多，这意味着 DPC 大量产生这些细胞外基质。在 sillicob 中，对基因进行比较，确定了对照 DPC 特异性基因和秃头 DPC 特异性基因，所有这些基因都在真皮乳头细胞中特异性表达。最后我们选择了8个候选基因，目前正在通过开发这些基因的敲除动物来研究其表达谱和功能。(2)。检查毛囊中甲状腺激素受体的表达。组成毛囊的所有细胞均为 T3 受体阳性。此外，T3对培养毛囊细胞生长的活性在生理浓度下最高。(3)．获得了针对人真皮乳头的单克隆抗体。其中一个被证实能够识别一种新的角蛋白。