Evolution of phytochrome signalling and nuclear transport

光敏色素信号传导和核运输的进化

• 批准号: 80790529
• 负责人: Professor Dr. Andreas Hiltbrunner
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/80790529?language=en
• 关键词: Evolution; phytochrome; signalling; nuclear; transport

In seed plants the phytochrome A (phyA) photoreceptor plays a key role in survival in far-red light enriched environments and it is essential for responses to very low intensities of light. After activation by light phyA binds to FHY1, which mediates phyA nuclear transport and accumulation. In contrast, cryptogam phys are believed to be cytosolic and/or membrane associated proteins. Although the split of the phy gene lineage into phyA and other phys occurred only in seed plants FHY1 like proteins can be identified in ferns, mosses and green algae, which do not contain phyA like phys. All these cryptogam FHY1 proteins interact with phyA as well as with cryptogam phys. Interestingly, also other key components of phyA signalling are represented in the Physcomitrella genome suggesting that phyA may have adopted a pre-existing signalling system from cryptogams and that also cryptogam phys have a function in the nucleus. Transgenic Physcomitrella lines expressing YFP tagged phys will be employed to test this hypothesis. Moreover, the FHY1 binding site of phyA/cryptogam phys will be mapped using a random mutagenesis/Y2H approach, which will provide further insight into how phyA signalling and the phy/FHY1 system evolved.

在种子植物中，植物色素A（PHYA）感光器在远红色光富集环境中的生存中起关键作用，对于对非常低的光强度的响应至关重要。光PHYA激活后与Fhy1结合，介导了Phya核转运和积累。相反，据信加密物理是胞质和/或膜相关的蛋白质。尽管PHY基因谱系分为Phya，而其他物理仅在种子植物中发生在类似蛋白质的种子植物中，可以在蕨类植物，苔藓和绿藻中鉴定出不包含Phya的phya。所有这些CryptoGam Fhy1蛋白与PHYA以及加密甘油疗法相互作用。有趣的是，Phya信号传导的其他关键组成部分也在Physcomitrella基因组中表示，这表明PHYA可能已经从加密蛋白酶中采用了先前存在的信号系统，并且Cryptogam Phys在细胞核中也具有功能。表达YFP标记的物理的转基因物理学系将用于检验该假设。此外，将使用随机诱变/Y2H方法对Phya/Cryptogam Phys的Fhy1结合位点进行映射，该方法将进一步了解PHYA信号传导和PHY/FHY1系统如何发展。