Functional characterisation of COR27 and COR28, two novel cold-induced phytochrome- and COP1/SPA binding proteins

两种新型冷诱导光敏色素和 COP1/SPA 结合蛋白 COR27 和 COR28 的功能表征

• 批准号: 326103268
• 负责人: Professor Dr. Andreas Hiltbrunner
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326103268?language=en
• 关键词: Functional; characterisation; COR27; COR28; two

Light and temperature are important environmental factors that determine plant growth and development. Several transcription factors involved in positive regulation of photomorphogenesis and induction of flowering are targets of the COP1/SPA E3 ubiquitin ligase. The activity of the COP1/SPA complex is downregulated by light-activated phytochromes and cryptochromes and recent reports show that COP1 is also controlled by temperature. We recently identified two novel phytochrome interacting proteins, COR27 and COR28. Expression of COR27 and COR28 is strongly enhanced by cold and transcriptomic data suggest that they might be regulated by autonomous pathway genes, HOS1, and the MYB/bHLH/WDR complex, which are involved in control of flowering, cold responses, and anthocyanin biosynthesis, respectively. COR27 and COR28 interact with COP1/SPA but appear not to be targets for their E3 ubiquitin ligase activity, suggesting instead that they might regulate the activity of the COP1/SPA complex in response to light and temperature. Thereby, COR27 and COR28 might contribute to light and temperature signal integration, which is important for the adaptation of growth and development to complex and rapidly changing environments. The cor27 cor28 double mutant is late flowering and seedlings overexpressing COR27 or COR28 accumulate increased levels of anthocyanin. In the proposed project we want to explore the molecular mechanisms involved in light and temperature regulation of COR27 and COR28, and investigate, how COR27 and COR28 together with COP1/SPA and phytochromes integrate light and temperature signals to regulate flowering, anthocyanin biosynthesis and cold responses.

光和温度是决定植物生长发育的重要环境因素。 COP1/SPA E3 泛素连接酶的靶标涉及光形态发生和诱导开花的正向调节的几种转录因子。 COP1/SPA 复合物的活性受到光激活光敏色素和隐花色素的下调，最近的报告表明 COP1 也受温度控制。我们最近鉴定了两种新型光敏色素相互作用蛋白：COR27 和 COR28。 COR27 和 COR28 的表达在寒冷条件下得到强烈增强，转录组数据表明它们可能受到自主途径基因 HOS1 和 MYB/bHLH/WDR 复合体的调节，这些基因参与控制开花、寒冷反应和花青素生物合成。分别。 COR27 和 COR28 与 COP1/SPA 相互作用，但似乎不是其 E3 泛素连接酶活性的靶标，这表明它们可能调节 COP1/SPA 复合物响应光和温度的活性。因此，COR27和COR28可能有助于光和温度信号集成，这对于生长和发育适应复杂和快速变化的环境非常重要。 cor27 cor28 双突变体开花晚，过度表达 COR27 或 COR28 的幼苗积累的花青素水平增加。在拟议的项目中，我们希望探索COR27和COR28参与光和温度调节的分子机制，并研究COR27和COR28如何与COP1/SPA和光敏色素一起整合光和温度信号来调节开花、花青素生物合成和冷反应。