Functional characterisation of COR27 and COR28, two novel cold-induced phytochrome- and COP1/SPA binding proteins

两种新型冷诱导光敏色素和 COP1/SPA 结合蛋白 COR27 和 COR28 的功能表征

• 批准号: 326103268
• 负责人: Professor Dr. Andreas Hiltbrunner
• 金额: --
• 依托单位: Institut für Biologie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326103268?language=en
• 关键词: Functional; characterisation; COR27; COR28; two

Light and temperature are important environmental factors that determine plant growth and development. Several transcription factors involved in positive regulation of photomorphogenesis and induction of flowering are targets of the COP1/SPA E3 ubiquitin ligase. The activity of the COP1/SPA complex is downregulated by light-activated phytochromes and cryptochromes and recent reports show that COP1 is also controlled by temperature. We recently identified two novel phytochrome interacting proteins, COR27 and COR28. Expression of COR27 and COR28 is strongly enhanced by cold and transcriptomic data suggest that they might be regulated by autonomous pathway genes, HOS1, and the MYB/bHLH/WDR complex, which are involved in control of flowering, cold responses, and anthocyanin biosynthesis, respectively. COR27 and COR28 interact with COP1/SPA but appear not to be targets for their E3 ubiquitin ligase activity, suggesting instead that they might regulate the activity of the COP1/SPA complex in response to light and temperature. Thereby, COR27 and COR28 might contribute to light and temperature signal integration, which is important for the adaptation of growth and development to complex and rapidly changing environments. The cor27 cor28 double mutant is late flowering and seedlings overexpressing COR27 or COR28 accumulate increased levels of anthocyanin. In the proposed project we want to explore the molecular mechanisms involved in light and temperature regulation of COR27 and COR28, and investigate, how COR27 and COR28 together with COP1/SPA and phytochromes integrate light and temperature signals to regulate flowering, anthocyanin biosynthesis and cold responses.

光和温度是决定植物生长和发育的重要环境因素。涉及光形态发生和诱导开花阳性的几个转录因子是COP1/SPA E3泛素连接酶的靶标。 COP1/SPA复合物的活性被光活化的植物色素和加密染色体下调，最近的报告表明，COP1也受温度控制。我们最近确定了两种新型的植物色素相互作用蛋白COR27和COR28。 COR27和COR28的表达通过冷和转录组数据强烈增强，表明它们可能受自主途径基因，HOS1和MYB/BHLH/WDR复合物的调节，这些基因，HOS1和MYB/BHLH/WDR复合物分别涉及开花，冷反应和羊植酸生物合成。 COR27和COR28与COP1/SPA相互作用，但似乎不是其E3泛素连接酶活性的靶标，相反，它们可能会根据光和温度来调节COP1/SPA复合物的活性。因此，COR27和COR28可能有助于光和温度信号整合，这对于适应生长和发展对复杂且快速变化的环境很重要。 COR27 COR28双重突变体是晚期开花，过表达COR27或COR28的幼苗会累积的花色苷水平增加。在拟议的项目中，我们希望探索COR27和COR28的光和温度调节所涉及的分子机制，并研究COR27和COR28与COP1/SPA和植物色素的COR27和COR28如何整合光和温度信号以调节开花，鸟类素的生物合成和冷反应。